LOCUS       sequence-412381-        8147 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To generate stable cell line expressing Halo-human MRE11(WT) by 
            Retroviral infection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8147)
  AUTHORS   Cho MG, Kumar RJ, Lin CC, Boyer JA, Shahir JA, Fagan-Solis K, 
            Simpson DA, Fan C, Foster CE, Goddard AM, Lerner LM, Ellington SW, 
            Wang Q, Wang Y, Ho AY, Liu P, Perou CM, Zhang Q, McGinty RK, Purvis 
            JE, Gupta GP
  TITLE     MRE11 liberates cGAS from nucleosome sequestration during 
            tumorigenesis.
  JOURNAL   Nature. 2024 Jan 10. doi: 10.1038/s41586-023-06889-6.
  PUBMED    38200309
REFERENCE   2  (bases 1 to 8147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2024 Jan 10. doi: 10.1038/s41586-023-06889-6."
COMMENT     Sequence Label: pBABE-puro-Halo-hMRE11(wt)
FEATURES             Location/Qualifiers
     source          1..8147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      269..857
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     758..777
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1011..1028
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             1158..1627
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(1848..2447)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1931..1951)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2428..2447
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(2457..2786)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2471..2606
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2771..2791
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(4946..4966)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             complement(4979..5869)
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     regulatory      5866..5875
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(5910..6326)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(5926..5942)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(5958..5980)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    6527..6726
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             6791..7260
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     promoter        7291..7395
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7396..7464,7465..8147,1..109)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7614..7633)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
       61 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
      121 agtttactca tatatacttt agattgattt gcggccgcaa aaggatctag gtgaagatcc
      181 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
      241 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
      301 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
      361 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
      421 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
      481 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
      541 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
      601 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
      661 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
      721 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
      781 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
      841 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
      901 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
      961 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     1021 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     1081 ttcattaatg caactatggc catttaatgt aaatacttaa gaaaaaaaac caaattaatt
     1141 ttgatacatg ctgcatgtga aagacccccg ctgacgggta gtcaatcact cagaggagac
     1201 cctcccaagg aacagcgaga ccacaagtcg gatgcaactg caagagggtt tattggatac
     1261 acgggtaccc gggcgactca gtcaatcgga ggactggcgc cccgagtgag gggttgtggg
     1321 ctcttttatt gagctcgggg agcagaagcg cgcgaacaga agcgagaagc gaactgattg
     1381 gttagttcaa ataaggcaca gggtcatttc aggtccttgg ggcaccctgg aaacatctga
     1441 tggttctcta gaaactgctg agggctggac cgcatctggg gaccatctgt tcttggccct
     1501 gagccggggc aggaactgct taccacagat atcctgtttg gcccatattc agctgttcca
     1561 tctgttcttg gccctgagcc ggggcaggaa ctgcttacca cagatatcct gtttggccca
     1621 tattcagctg ttccatctgt tcctgacctt gatctgaact tctctattct cagttatgta
     1681 tttttccatg ccttgcaaaa tggcgttact taagctagct tgccaaacct acaggtgggg
     1741 tctttcattc cccccttttt ctggagacta aataaaatct tttattttat cgatgcatgg
     1801 ggtcgtgcgc tcctttcggt cgggcgctgc gggtcgtggg gcgggcgtca ggcaccgggc
     1861 ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg
     1921 aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg aggtctccag gaaggcgggc
     1981 accccggcgc gctcggccgc ctccactccg gggagcacga cggcgctgcc cagacccttg
     2041 ccctggtggt cgggcgagac gccgacggtg gccaggaacc acgcgggctc cttgggccgg
     2101 tgcggcgcca ggaggccttc catctgttgc tgcgcggcca gccgggaacc gctcaactcg
     2161 gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc
     2221 cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct tgccgatgtc gagcccgacg
     2281 cgcgtgagga agagttcttg cagctcggtg acccgctcga tgtggcggtc cggatcgacg
     2341 gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga gggtgcgtac ggccctgggg
     2401 acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact cggtcatggt aagctttttg
     2461 caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag
     2521 gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg
     2581 gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac
     2641 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
     2701 ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
     2761 gactttccac accctaactg acacacattc cacagggtcg actgcattat cttctatttc
     2821 ttcttaaaga actagtgttc ataaaaggat catcatcatc atcctcactt gattcaaaat
     2881 caaccccttt cgatacttga ctctgggaca tgattttgct ggatgatgtg ctggaccacc
     2941 tttgatctgt ctttgaagtg gtaggaaaaa tgtcttcttc cacatctgat tcatctacct
     3001 caatcacctc tgaataattc ttagtagtga catttcggga aggctgctgt cttgtagatt
     3061 taaaggcatc tataatagac atatttctag atgctgacac agcagtcttt gagttcctgc
     3121 tacgggtaga agtctccaga ccagtgtctg ctcttcctct ttgagaccct cctctcgatg
     3181 ctgaattctg ccctcttcca cctcttcgac ctcttcctcg gcctcttcct ttgttggttg
     3241 ctgctgagat gctatcatca gagtcattag ccatctgttc tgctaaatct atactcataa
     3301 ggtcatcagc actaaaggca gaagcagact cctctgactg agatctgagt gctctggccc
     3361 tggtcatagc ctcacggact tcatcatctt cttcattagt atttttttgt ctggtttctc
     3421 tgaaacgacg tacctcctca tcgattttgt cttcgagggc atcaatgtga cgttctttaa
     3481 gaaatcgctg tgttttttcc aactggtatt tcactaattc ctcaatggca tctttctcct
     3541 ccttgtccac aaattcttgt actgcttcac ccatccctct ttctgttagc agtgagagct
     3601 gcacattctt ctctgcggtt tgaaagtact gttttacaag atcttctacc cttaaagttg
     3661 ttccttctga aggctttgtg ataagtttcc caaagttgat ctcttctcct gttttttcct
     3721 tttgttctct atgcctgaaa aaatggataa tgtcttttgg attagctacc cgatccacaa
     3781 atttctggct aaagcgaaga acactgaaag gttcaaaacc tccactatag tccactcgca
     3841 gtcgtacaag aggcttctct ggctggtgag aattacccag acgttcccgt tcagcatttt
     3901 caagcatttc ttcaatcttc tccaaacaga agctttgtat ggcttgggtt actttaggat
     3961 tatctgggtt aaaaatgtct ggatgattag ctagaacaat atcctccatg aaaaactgcc
     4021 gcactgtgtg aagaggaatt ttatgcatat tcatcttcct ccctttaata cgcagcaaac
     4081 caacatgttt ctttacagct tctcctgggg aaagagaagt aaccactgag cttccaggtt
     4141 gtgagatata aaacagctgt tgttcatttt tggttggagc tattttacac tcatgttcat
     4201 ggccccagat aacaagatca atgaagtcat ccaaaaattg ttctggaatg aagttagtac
     4261 ttccatgttt actcctgttc tgatgaatca caaataagtt aaaccaagag ttctcatctt
     4321 cctttggtct caacattgtt acttttttat tgacaaacat tcgatagagc ctttcatctg
     4381 gaatggatcc taaaccatat agcgcaatct ttgtgcttcc tttttgaagc aaaaccggac
     4441 taatgtctat cttctccaca gacattgaac gtccaaagtg atttacaaat ccagcacaac
     4501 ttaaaatgtc caaggcacaa agtgcatctg cccctgtggg atcgtcatga ttgccatgaa
     4561 tactaaacac tggaattgaa atgttgaggt tgccatcttg atagttcacc catggaaact
     4621 tactaaaacc aaagttgact gactgatcac tgagaatttc aaactggaca ggccgatcac
     4681 ccatacaata ttttcttaat aactcgaggc aggtatgtaa tgttttcctt gagggcttat
     4741 tttcatgaaa aagatcacca cctaacaaaa taaaatccac ttcattttcc tgggcaagtc
     4801 ttaaaatttc atcgagtgtt acaaacgtat catttcctct gactgcatct ttctccataa
     4861 atccaagatg aatatctgtt gcaactaata ttttaaatgt gttttcatca tcaagtgcat
     4921 ctgcagtact catagatctg ttatcgctct gaaagtacag atcctcagtg gttggctcac
     4981 cggaaatctc cagagtagac agccagcgcg cgatctcgct gccgatcagg tccgggttgt
     5041 cttcttgcag cagattcaga cccgggccga tgtccacagc cttgcagtta ggcaggcttt
     5101 tggccaggcg agcggcttcg gccggtggga tcagaacgcc tggggtgccc cagaacagca
     5161 gcttcgggac aggggactgg tgcagccagt ccatgtattc ttcgaccagc gcgacgatgt
     5221 tcgctggctc accggcgatt ggcagctcgt ttgggaagcg ccacagtggc tcgcggtcaa
     5281 caggattcag gaacggctcg cggtaatggt ccatctcgac ttcagtcagc gggcggacga
     5341 cacccatcgg cagcgtaccc tcgataaaaa cgttctgatc gatgatcagc ttgcggccga
     5401 cgtcggtggt gcggaaggcc tggaaggtct cgcgggcaaa ttctggccat tcgtcccagg
     5461 tcgggatagg gcggatgaac tccataaatg caataccttt gacgcgctct ggattgcgct
     5521 tggcccagtg gaaacccaga gcggagcccc agtcgtgaat gaccaggacg acctcttcca
     5581 gacccagggc ttcgatgaag gcatccatga agcggacgtg gtcgtcgaag aaataaccca
     5641 ggtctggttt gtcggatttg cccataccga tcaggtctgg agcaatgcag cgatgggtcg
     5701 gtgcaacatg cgggatgatg ttgcgccaca cgtaggagga ggtcgggtta ccgtgcagga
     5761 acagcacagg ggtgccatcg cgcggaccaa catcgacgta gtgcatgcgc tcgcccagga
     5821 cttccacata atgggggtcg aatggaaagc cagtaccgat ttctgccatg gtggcgaccg
     5881 gtagcgtacc accacactgg gatccggccg gcgcctagag aaggagtgag ggctggataa
     5941 agggaggatc gaggcggggt cgaacgagga ggttcaaggg ggagagacgg ggcggatgga
     6001 ggaagaggag gcggaggctt agggtgtaca aagggcttga cccagggagg ggggtcaaaa
     6061 gccaaggctt cccaggtcac gatgtagggg acctggtctg ggtgtccatg cgggccaggt
     6121 gaaaagacct tgatcttaac ctgggtgatg aggtctcggt taaaggtgcc gtctcgcggc
     6181 catccgacgt taaaggttgg ccattctgca gagcagaagg taacccaacg tctcttcttg
     6241 acatctaccg actggttgtg agcgatccgc tcgacatctt tccagtgacc taaggtcaaa
     6301 cttaagggag tggtaacagt ctggccctaa ttttcagaca aatacagaaa cacagtcaga
     6361 cagagacaac acagaacgat gctgcagcgc tgcagcagac aagacgcgcg gcttcggttc
     6421 caaaccgaaa gcaaaaattc agacggaggc gggaactgtt ttaggttctc gtctcctacc
     6481 agaaccacat atcctgacgg ggtcggattc cacatcgact cccttcctca ggtcgggcca
     6541 caaaaacggc ccccaaagtc cctgggacgt ctcccagggt tgcggccggg tgttcagaac
     6601 tcgtcagttc caccacgggt ccgccagata cagagctagt tagctaacta gtaccgacgc
     6661 aggcgcataa aatcagtcat agacactaga caatcggaca gacacagata agttgctggc
     6721 cagcttgcct cccggtggtg ggtcggtggt ccctgggcag gggtctcccg atcccggacg
     6781 agcccccaaa tgaaagaccc ccgctgacgg gtagtcaatc actcagagga gaccctccca
     6841 aggaacagcg agaccacaag tcggatgcaa ctgcaagagg gtttattgga tacacgggta
     6901 cccgggcgac tcagtcaatc ggaggactgg cgccccgagt gaggggttgt gggctctttt
     6961 attgagctcg gggagcagaa gcgcgcgaac agaagcgaga agcgaactga ttggttagtt
     7021 caaataaggc acagggtcat ttcaggtcct tggggcaccc tggaaacatc tgatggttct
     7081 ctagaaactg ctgagggctg gaccgcatct ggggaccatc tgttcttggc cctgagccgg
     7141 ggcaggaact gcttaccaca gatatcctgt ttggcccata ttcagctgtt ccatctgttc
     7201 ttggccctga gccggggcag gaactgctta ccacagatat cctgtttggc ccatattcag
     7261 gctgcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     7321 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     7381 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     7441 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     7501 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     7561 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     7621 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     7681 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     7741 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     7801 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     7861 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     7921 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     7981 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     8041 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     8101 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatgg
//
