LOCUS       sequence-412382-       11021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To generate stable cell line expressing human cGAS(R255A) using 
            PiggyBac transposon system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11021)
  AUTHORS   Cho MG, Kumar RJ, Lin CC, Boyer JA, Shahir JA, Fagan-Solis K, 
            Simpson DA, Fan C, Foster CE, Goddard AM, Lerner LM, Ellington SW, 
            Wang Q, Wang Y, Ho AY, Liu P, Perou CM, Zhang Q, McGinty RK, Purvis 
            JE, Gupta GP
  TITLE     MRE11 liberates cGAS from nucleosome sequestration during 
            tumorigenesis.
  JOURNAL   Nature. 2024 Jan 10. doi: 10.1038/s41586-023-06889-6.
  PUBMED    38200309
REFERENCE   2  (bases 1 to 11021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2024 Jan 10. doi: 10.1038/s41586-023-06889-6."
COMMENT     Sequence Label: PB-TA-ERP2-hcGAS(R255A)
FEATURES             Location/Qualifiers
     source          1..11021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    247..265
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    284..302
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    321..339
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    358..376
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    395..413
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    432..450
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    469..487
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    506..524
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     546..570
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    649..673
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2246..2270)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2385..2404)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2450..2505
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2504..2523)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     enhancer        2948..3327
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3442..4747
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    3784..4715
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     CDS             join(4764..5381,5382..5387,5388..5423,5424..5426,5427..5462,
                     5463..5465,5466..5501,5502..5510)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    join(5537..6111,6112..6114,6115..6123)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5704..5721)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5931..5950
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6124..6723
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6124..6143)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6620..6640
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6809..7381
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
     repeat_region   7755..7789
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(7938..7956)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7977..7993)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7977..7993)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7990..8012)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8001..8017
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8025..8031,8032..8049,8050..8055))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8070..8091
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8208..8225)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8379..8967)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8459..8478)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9138..9929,9930..9998))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9761..9780
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9999..10103)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10129..10584)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10266..10287)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10478..10497
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10711..10733
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10725..10742
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10726..10742
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10749..10768
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10749..10767
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   10834..10896
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 aaaaaaaaac aaaaactcaa aatttcttct ataaagtaac aaaactttta aacattctct
       61 cttttacaaa aataaactta ttttgtactt taaaaacagt catgttgtat tataaaataa
      121 gtaattagct taacctatac ataatagaaa caaattatac ttattagtca gtcagaaaca
      181 actttggcac atatcaatat tatgctctcg ttaattaact cgagggatcc aagctggccg
      241 cacgtctccc tatcagtgat agagaagtcg acacgtctcg agctccctat cagtgataga
      301 gaaggtacgt ctagaacgtc tccctatcag tgatagagaa gtcgacacgt ctcgagctcc
      361 ctatcagtga tagagaaggt acgtctagaa cgtctcccta tcagtgatag agaagtcgac
      421 acgtctcgag ctccctatca gtgatagaga aggtacgtct agaacgtctc cctatcagtg
      481 atagagaagt cgacacgtct cgagctccct atcagtgata gagaaggtac cccctatata
      541 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
      601 ctccatagaa gacaccggga ccgatccagc ctccgcggtg gcggcatcac aagtttgtac
      661 aaaaaagcag gcttcatgca gccttggcac ggaaaggcca tgcagagagc ttccgaggcc
      721 ggagccactg cccccaaggc ttccgcacgg aatgccaggg gcgccccgat ggatcccaac
      781 gagtctccgg ctgcccccga ggccgccctg cctaaggcgg gaaagttcgg ccccgccagg
      841 aagtcgggat cccggcagaa aaagagcgcc ccggacaccc aggagaggcc gcccgtccgc
      901 gcaactgggg cccgcgccaa aaaggcccct cagcgcgccc aggacacgca gccgtctgac
      961 gccaccagcg cccctggggc agaggggctg gagcctcctg cggctcggga gccggctctt
     1021 tccagggctg gttcttgccg ccagaggggc gcgcgctgct ccacgaagcc aagacccccg
     1081 cccgggccct gggacgtgcc cagccccggc ctgccggtct cggcccccat tctcgtacgg
     1141 agggatgcgg cgcctggggc ctcgaagctc cgggcggttt tggagaagtt gaagctcagc
     1201 cgcgatgata tctccacggc ggcggggatg gtgaaagggg ttgtggacca cctgctgctc
     1261 agactgaagt gcgactccgc gttcagaggc gtcgggctgc tgaacaccgg gagctactat
     1321 gagcacgtga agatttctgc acctaatgaa tttgatgtca tgtttaaact ggaagtcccc
     1381 agaattcaac tagaagaata ttccaacact cgtgcatatt actttgtgaa atttaaagca
     1441 aatccgaaag aaaatcatct gagtcagttt ttagaaggtg aaatattatc agcttctaag
     1501 atgctgtcaa agtttaggaa aatcattaag gaagaaatta acgacattaa agatacagat
     1561 gtcatcatga agaggaaaag aggagggagc cctgctgtaa cacttcttat tagtgaaaaa
     1621 atatctgtgg atataaccct ggctttggaa tcaaaaagta gctggcctgc tagcacccaa
     1681 gaaggcctgc gcattcaaaa ctggctttca gcaaaagtta ggaagcaact acgactaaag
     1741 ccattttacc ttgtacccaa gcatgcaaag gaaggaaatg gtttccaaga agaaacatgg
     1801 cggctatcct tctctcacat cgaaaaggaa attttgaaca atcatggaaa atctaaaacg
     1861 tgctgtgaaa acaaagaaga gaaatgttgc aggaaagatt gtttaaaact aatgaaatac
     1921 cttttagaac agctgaaaga aaggtttaaa gacaaaaaac atctggataa attctcttct
     1981 tatcatgtga aaactgcctt ctttcacgta tgtacccaga accctcaaga cagtcagtgg
     2041 gaccgcaaag acctgggcct ctgctttgat aactgcgtga catactttct tcagtgcctc
     2101 aggacagaaa aacttgagaa ttattttatt cctgaattca atctattctc tagcaactta
     2161 attgacaaaa gaagtaaaga atttctgaca aagcaaattg aatatgaaag aaacaatgag
     2221 tttccagttt ttgatgaatt ttgaaaccca gctttcttgt acaaagtggt gatggccgct
     2281 ctagcgaagg gatcccccat cgaattcact cctcaggtgc aggctgccta tcagaaggtg
     2341 gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt tccctctgcc
     2401 aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa
     2461 tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat
     2521 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
     2581 gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa
     2641 cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt
     2701 tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt
     2761 ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc
     2821 ttatggagat ccctcgacct gcaggaattc gattacttaa gcctaggatc cagatctgta
     2881 cattcgaatc gatgaattcg ttaaccatgg aagcttctgc agcggccgca ctagcggtgg
     2941 cggcctcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     3001 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     3061 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     3121 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     3181 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     3241 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     3301 tacgtattag tcatcgctat taccatgatg atgcggtttt ggcagtacat caatgggcgt
     3361 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     3421 ttgttttgac tagtggagcc gagagtaatt catacaaaag gagggatcgc cttcgcaagg
     3481 ggagagccca gggaccgtcc ctaaattctc acagacccaa atccctgtag ccgccccacg
     3541 acagcgcgag gagcatgcgc ccagggctga gcgcgggtag atcagagcac acaagctcac
     3601 agtccccggc ggtgggggga ggggcgcgct gagcgggggc cagggagctg gcgcggggca
     3661 aactgggaaa gtggtgtcgt gtgctggctc cgccctcttc ccgagggtgg gggagaacgg
     3721 tatataagtg cggtagtcgc cttggacgtt ctttttcgca acgggtttgc cgtcagaacg
     3781 caggtgagtg gcgggtgtgg cttccgcggg ccccggagct ggagccctgc tctgagcggg
     3841 ccgggctgat atgcgagtgt cgtccgcagg gtttagctgt gagcattccc acttcgagtg
     3901 gcgggcggtg cgggggtgag agtgcgaggc ctagcggcaa ccccgtagcc tcgcctcgtg
     3961 tccggcttga ggcctagcgt ggtgtccgcc gccgcgtgcc actccggccg cactatgcgt
     4021 tttttgtcct tgctgccctc gattgccttc cagcagcatg ggctaacaaa gggagggtgt
     4081 ggggctcact cttaaggagc ccatgaagct tacgttggat aggaatggaa gggcaggagg
     4141 ggcgactggg gcccgcccgc cttcggagca catgtccgac gccacctgga tggggcgagg
     4201 cctgtggctt tccgaagcaa tcgggcgtga gtttagccta cctgggccat gtggccctag
     4261 cactgggcac ggtctggcct ggcggtgccg cgttcccttg cctcccaaca agggtgaggc
     4321 cgtcccgccc ggcaccagtt gcttgcgcgg aaagatggcc gctcccgggg ccctgttgca
     4381 aggagctcaa aatggaggac gcggcagccc ggtggagcgg gcgggtgagt cacccacaca
     4441 aaggaagagg gccttgcccc tcgccggccg ctgcttcctg tgaccccgtg gtctatcggc
     4501 cgcatagtca cctcgggctt ctcttgagca ccgctcgtcg cggcgggggg aggggatcta
     4561 atggcgttgg agtttgttca catttggtgg gtggagacta gtcaggccag cctggcgctg
     4621 gaagtcattc ttggaatttg cccctttgag tttggagcga ggctaattct caagcctctt
     4681 agcggttcaa aggtattttc taaacccgtt tccaggtgtt gtgaaagcca ccgctaattc
     4741 aaagcaatcc ggagtaattc accatgtcta gactggacaa gagcaaagtc ataaacggcg
     4801 ctctggaatt actcaatgga gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa
     4861 agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg gccctgctcg
     4921 atgccctgcc aatcgagatg ctggacaggc atcataccca cttctgcccc ctggaaggcg
     4981 agtcatggca agactttctg cggaacaacg ccaagtcatt ccgctgtgct ctcctctcac
     5041 atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag tacgaaaccc
     5101 tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac gcactgtacg
     5161 ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag gagcatcaag
     5221 tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt ctgagacaag
     5281 caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc ctggaactaa
     5341 tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccggcc gacgcccttg
     5401 acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac cttgatatgc
     5461 tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa ctaagtaagg
     5521 atcattccgc cccccccccc tctccctccc ccccccctaa cgttactggc cgaagccgct
     5581 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     5641 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     5701 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     5761 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     5821 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     5881 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     5941 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     6001 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     6061 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca
     6121 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     6181 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     6241 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     6301 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     6361 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     6421 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     6481 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     6541 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     6601 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     6661 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     6721 tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg
     6781 aaatgaccga ccaagcgaat tcgctaggat tatccctaat acctgccacc ccactcttaa
     6841 tcagtggtgg aagaacggtc tcagaactgt ttgtttcaat tggccattta agtttagtag
     6901 taaaagactg gttaatgata acaatgcatc gtaaaacctt cagaaggaaa ggagaatgtt
     6961 ttgtggacca ctttggtttt cttttttgcg tgtggcagtt ttaagttatt agtttttaaa
     7021 atcagtactt tttaatggaa acaacttgac caaaaatttg tcacagaatt ttgagaccca
     7081 ttaaaaaagt taaatgagaa acctgtgtgt tcctttggtc aacaccgaga catttaggtg
     7141 aaagacatct aattctggtt ttacgaatct ggaaacttct tgaaaatgta attcttgagt
     7201 taacacttct gggtggagaa tagggttgtt ttccccccac ataattggaa ggggaaggaa
     7261 tatcatttaa agctatggga gggttgcttt gattacaaca ctggagagaa atgcagcatg
     7321 ttgctgattg cctgtcacta aaacaggcca aaaactgagt ccttgggttg catagaaagc
     7381 tgccaaacgc tagccaacaa gctcgtcatc gctttgcaga agagcagaga ggatatgctc
     7441 atcgtctaaa gaactaccca ttttattata tattagtcac gatatctata acaagaaaat
     7501 atatatataa taagttatca cgtaagtaga acatgaaata acaatataat tatcgtatga
     7561 gttaaatctt aaaagtcacg taaaagataa tcatgcgtca ttttgactca cgcggtcgtt
     7621 atagttcaaa atcagtgaca cttaccgcat tgacaagcac gcctcacggg agctccaagc
     7681 ggcgactgag atgtcctaaa tgcacagcga cggattcgcg ctatttagaa agagagagca
     7741 atatttcaag aatgcatgcg tcaattttac gcagactatc tttctagggt taaaaaagat
     7801 ttgcgcttta ctcgacctaa actttaaaca cgtcatagaa tcttcgtttg acaaaaacca
     7861 cattgtggcc aagctgtgtg acgcgacgcg cgctaaagaa tggcaaacca agtcgcgcga
     7921 ggtaccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca
     7981 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     8041 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8101 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8161 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8221 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8281 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8341 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8401 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8461 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8521 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8581 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8641 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8701 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8761 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     8821 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8881 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8941 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9001 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9061 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9121 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9181 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9241 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9301 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9361 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9421 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9481 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9541 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9601 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9661 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9721 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9781 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9841 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9901 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9961 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10021 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10081 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
    10141 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
    10201 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
    10261 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
    10321 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
    10381 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
    10441 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
    10501 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
    10561 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
    10621 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
    10681 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
    10741 gtgaattgta atacgactca ctatagggcg aattggagct cggtattcac gacagcaggc
    10801 tgaataataa aaaaattaga aactattatt taaccctaga aagataatca tattgtgacg
    10861 tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
    10921 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
    10981 ataaattcaa caaacaattt atttatgttt atttatttat t
//
