LOCUS       sequence-412463-       10527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting 
            murine ATM gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10527)
  AUTHORS   Cho MG, Kumar RJ, Lin CC, Boyer JA, Shahir JA, Fagan-Solis K, 
            Simpson DA, Fan C, Foster CE, Goddard AM, Lerner LM, Ellington SW, 
            Wang Q, Wang Y, Ho AY, Liu P, Perou CM, Zhang Q, McGinty RK, Purvis 
            JE, Gupta GP
  TITLE     MRE11 liberates cGAS from nucleosome sequestration during 
            tumorigenesis.
  JOURNAL   Nature. 2024 Jan 10. doi: 10.1038/s41586-023-06889-6.
  PUBMED    38200309
REFERENCE   2  (bases 1 to 10527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2024 Jan 10. doi: 10.1038/s41586-023-06889-6."
COMMENT     Sequence Label: Lenti-sgRNA-Cre-GpNLuc-sgmATM
FEATURES             Location/Qualifiers
     source          1..10527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     228..248
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(257..685)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(367..388)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     579..598
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    731..955
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     944..961
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             987..1220
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    1292..1880
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1398..1409
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1807..1827
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1896..2411)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             complement(join(2427..3137,3138..3140,3141..3143))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2427..3137,3138..3140,3141..3143))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2469..2490)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     2818..2837
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             complement(3144..3200)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(3201..4226)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLQ
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     CDS             complement(4227..4247)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(4273..4484)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_RNA        complement(4546..4621)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(4643..4891)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(4701..4720)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(4871..4891)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    4942..5059
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5357..5401)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5586..5819
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6312..6437
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6484..6664
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6682..6880)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6707..6727)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6882..7261
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7433..7452
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7527..7631
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7632..7700,7701..8492)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7850..7869)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8663..9251
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9152..9171
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9405..9422
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9539..9560
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9575..9580,9581..9598,9599..9605)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9613..9629
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9618..9640
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9637..9653
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9637..9653
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    9702..9823
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9713..9732)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9767..9786
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(9953..10327)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(10346..10393)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(join(10441..10527,1..243))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(10455..10527,1..63)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(join(10509..10527,1))
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg
       61 gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat
      121 acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg
      181 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca
      241 cacattccac agaattaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
      301 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
      361 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
      421 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
      481 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
      541 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
      601 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
      661 cttaatgcgc cgctacaggg cgcgtgggga taccccctag agccccagct ggttctttcc
      721 gcctcagaag ccatagagcc caccgcatcc ccagcatgcc tgctattgtc ttcccaatcc
      781 tcccccttgc tgtcctgccc caccccaccc cccagaatag aatgacacct actcagacaa
      841 tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg gagtggcacc ttccagggtc
      901 aaggaaggca cgggggaggg gcaaacaaca gatggctggc aactagaagg cacagtcgag
      961 gctgatcagc gggtttaaac gggccctgct agagattttc cacactgact aaaagggtct
     1021 gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca
     1081 aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc
     1141 tcagatctgg tctaaccaga gagacccagt acaagcaaaa agcagatctt gtcttcgttg
     1201 ggagtgaatt agcccttcca gtcccccctt ttcttttaaa aagtggctaa gatctacagc
     1261 tgccttgtaa gtcattggtc ttaaagtcga cgcggggagg cggcccaaag ggagatccga
     1321 ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag caggccgcgg
     1381 gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt cccgcgcaga
     1441 atccaggtgg caacacaggc gagcagccaa ggaaaggacg atgatttccc cgacaacacc
     1501 acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag gcaggcggcg
     1561 atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag gagctgacag
     1621 gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca caccacgcca
     1681 cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga tttatacaag
     1741 gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat taaagcagcg
     1801 tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct ttcacaaatt
     1861 ttgtaatcca gaggttgatt gtcgacttaa cgcgtttacg ccagaatgcg ttcgcacagc
     1921 cgccagccgg tcactccgtt gatggttact cggaacagca gggagccgtc ggggttgatc
     1981 aggcgctcgt cgataatttt gttgccgttc cacagggtcc ctgttacagt gatctttttg
     2041 ccgtcgaaca cggcgatgcc ttcatacggc cgtccgaaat agtcgatcat gttcggcgta
     2101 accccgtcga ttaccagtgt gccatagtgc aggatcacct taaagtgatg atcatccaca
     2161 gggtacacca ccttaaaaat tttttcgatc tggcccattt ggtcgccgct cagaccttca
     2221 tacgggatga tgacatggat gtcgatcttc agcccatttt caccgctcag gacaatcctt
     2281 tggatcggag ttacggacac cccgagattc tgaaacaaac tggacacacc tccctgttca
     2341 aggacttggt ccaggttgta gccggctgtc tgtcgccagt ccccaacgaa atcttcgagt
     2401 gtgaagacca tacctccgga gatatccttg tacagctcgt ccatgccgag agtgatcccg
     2461 gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc
     2521 agggcggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg
     2581 ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg
     2641 atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg
     2701 ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg
     2761 cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc
     2821 ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg
     2881 gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg
     2941 ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag
     3001 atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg
     3061 aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac
     3121 agctcctcgc ccttgctcac catcggtcca ggattctctt cgacatctcc ggcttgtttc
     3181 agcagagaga agtttgttgc atcgccatct tccagcaggc gcaccattgc ccctgtttca
     3241 ctatccaggt tacggatata gttcatgaca atatttacat tggtccagcc accagcttgc
     3301 atgatctccg gtattgaaac tccagcgcgg gccatatctc gcgcggctcc gacacgggca
     3361 ctgtgtccag accaggccag gtatctctga ccagagtcat ccttagcgcc gtaaatcaat
     3421 cgatgagttg cttcaaaaat cccttccagg gcgcgagttg atagctggct ggtggcagat
     3481 ggcgcggcaa caccattttt tctgacccgg caaaacaggt agttattcgg atcatcagct
     3541 acaccagata cggaaatcca tcgctcgacc agtttagtta cccccaggct aagtgccttc
     3601 tctacacctg cggtgctaac cagcgttttc gttctgccaa tatggattaa cattctccca
     3661 ccgtcagtac gtgagatatc tttaaccctg atcctggcaa tttcggctat acgtaacagg
     3721 gtgttataag caatccccag aaatgccaga ttacgtatat cctggcagcg atcgctattt
     3781 tccatgagtg aacgaacctg gtcgaaatca gtgcgttcga acgctagagc ctgttttgca
     3841 cgttcaccgg catcaacgtt ttcttttcgg atccgccgca taaccagtga aacagcattg
     3901 ctgtcacttg gtcgtggcag cccggaccga cgatgaagca tgtttagctg gcccaaatgt
     3961 tgctggatag tttttactgc cagaccgcgc gcctgaagat atagaagata atcgcgaaca
     4021 tcttcaggtt ctgcgggaaa ccatttccgg ttattcaact tgcaccatgc cgcccacgac
     4081 cggcaaacgg actgaagcat tttccaggta tgctcagaaa acgcctggcg atccctgaac
     4141 atgtccatca ggttcttgcg aacctcatca ctcgttgcat cgaccggtaa tgcaggcaaa
     4201 ttttggtgta cggtcagtaa attggacacc ttcctcttct tcttgggcat ggtagatcct
     4261 ctagaaccgg tcctgtgttc tggcggcaaa cccgttgcga aaaagaacgt tcacggcgac
     4321 tactgcactt atatacggtt ctcccccacc ctcgggaaaa aggcggagcc agtacacgac
     4381 atcactttcc cagtttaccc cgcgccacct tctctaggca ccggatcaat tgccgacccc
     4441 tccccccaac ttctcgggga ctgtgggcga tgtgcgctct gcccactgac gggcaccgga
     4501 gccaattccc actcctttca agacctagct agcgaattca aaaaagcacc gactcggtgc
     4561 cactttttca agttgataac ggactagcct tattttaact tgctatttct agctctaaaa
     4621 ctgacataat tcaagaggtc tcggtgtttc gtcctttcca caagatatat aaagccaaga
     4681 aatcgaaata ctttcaagtt acggtaagca tatgatagtc cattttaaaa cataatttta
     4741 aaactgcaaa ctacccaaga aattattact ttctacgtca cgtattttgt actaatatct
     4801 ttgtgtttac agtcaaatta attctaatta tctctctaac agccttgtat cgtatatgca
     4861 aatatgaagg aatcatggga aataggccct cggtacctta attaaccaaa ctggatctct
     4921 gctgtccctg taataaaccc gaaaattttg aatttttgta atttgttttt gtaattcttt
     4981 agtttgtatg tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat
     5041 cccccctttt cttttaaaat tgtggatgaa tactgccatt tgtctgcaga attggcgcac
     5101 gcagtgccga tccgttcact aatcgaatgg atctgtctct gtctctctct ccaccttctt
     5161 cttctattcc ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata
     5221 atggtgaata tccctgccta actctattca ctatagaaag tacagcaaaa actattctta
     5281 aacctaccaa gcctcctact atcattatga ataattttat ataccacagc caatttgtta
     5341 tgttaaacca attccacaaa cttgcccatt tatctaattc caataattct tgttcattct
     5401 tttcttgctg gttttgcgat tcttcaatta aggagtgtat taagcttgtg taattgttaa
     5461 tttctctgtc ccactccatc caggtcgtgt gattccaaat ctgttccaga gatttattac
     5521 tccaactagc attccaaggc acagcagtgg tgcaaatgag ttttccagag caaccccaaa
     5581 tccccaggag ctgttgatcc tttaggtatc tttccacagc caggattctt gcctggagct
     5641 gcttgatgcc ccagactgtg agttgcaaca gatgctgttg cgcctcaata gccctcagca
     5701 aattgttctg ctgctgcact ataccagaca ataattgtct ggcctgtacc gtcagcgtca
     5761 ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa cccaaggaac aaagctccta
     5821 ttcccactgc tcttttttct ctctgcacca ctcttctctt tgccttggtg ggtgctactc
     5881 ctaatggttc aatttttact actttatatt tatataattc acttctccaa ttgtccctca
     5941 tatctcctcc tccaggtctg aagatcagcg gccgcttgct gtgcggtggt cttacttttg
     6001 ttttgctctt cctctatctt gtctaaagct tccttggtgt cttttatctc tatcctttga
     6061 tgcacacaat agagggttgc tactgtatta tataatgatc taagttcttc tgatcctgtc
     6121 tgaagggatg gttgtagctg tcccagtatt tgtctacagc cttctgatgt ttctaacagg
     6181 ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc atactatatg ttttaattta
     6241 tattttttct ttccccctgg ccttaaccga attttttccc atcgcgatct aattctcccc
     6301 cgcttaatac tgacgctctc gcacccatct ctctccttct agcctccgct agtcaaaatt
     6361 tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga
     6421 gtcctgcgtc gagagagctc ctctggtttc cctttcgctt tcaagtccct gttcgggcgc
     6481 cactgctaga gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt
     6541 cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa
     6601 gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag
     6661 acccagtaca ggcaaaacgc gctgcttata tagacctccc accgtacacg cctaccgccc
     6721 atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg attttggtgc
     6781 caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg tgagtcaaac
     6841 cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat agcgatgact
     6901 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc
     6961 caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact
     7021 tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa
     7081 gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg
     7141 ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat
     7201 gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt
     7261 caacgcgtat atctggcccg tacatcgcga agcagcgcaa aacgcctaac cctaagcaga
     7321 ttcttcatgc aattgtcggt caagccttgc cttgttgtag cttaaatttt gctcgcgcac
     7381 tactcagcga cctccaacac acaagcaggg agcagatact ggcttaacta tgcggcatca
     7441 gagcagattg tactgagagt gcaccatagg ggatcgggag atctcccgat ccgtcgacgt
     7501 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7561 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7621 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7681 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7741 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7801 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7861 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7921 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7981 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     8041 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     8101 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     8161 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     8221 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     8281 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     8341 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     8401 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8461 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8521 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8581 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8641 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8701 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8761 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     8821 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8881 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8941 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     9001 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     9061 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     9121 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     9181 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     9241 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     9301 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     9361 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     9421 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     9481 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     9541 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     9601 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     9661 acgccaagct ctagctagag gtcgacggta tacagacatg ataagataca ttgatgagtt
     9721 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     9781 tattgcttta tttgtaacca ttataagctg caataaacaa gttggggtgg gcgaagaact
     9841 ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga
     9901 agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtagcacg tgtcagtcct
     9961 gctcctcggc cacgaagtgc acgcagttgc cggccgggtc gcgcagggcg aactcccgcc
    10021 cccacggctg ctcgccgatc tcggtcatgg ccggcccgga ggcgtcccgg aagttcgtgg
    10081 acacgacctc cgaccactcg gcgtacagct cgtccaggcc gcgcacccac acccaggcca
    10141 gggtgttgtc cggcaccacc tggtcctgga ccgcgctgat gaacagggtc acgtcgtccc
    10201 ggaccacacc ggcgaagtcg tcctccacga agtcccggga gaacccgagc cggtcggtcc
    10261 agaactcgac cgctccggcg acgtcgcgcg cggtgagcac cggaacggca ctggtcaact
    10321 tggccatggt ttagttcctc accttgtcgt attatactat gccgatatac tatgccgatg
    10381 attaattgtc aacacgtgct gatcagatcc gaaaatggat atacaagctc ccgggagctt
    10441 tttgcaaaag cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc
    10501 agaggcggcc tcggcctctg cataaat
//
