LOCUS       sequence-412475-        6130 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SLIT1 protein expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6130)
  TITLE     TREAT-AD plasmids Jun 2023
REFERENCE   2  (bases 1 to 6130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SLIT1A-c003
FEATURES             Location/Qualifiers
     source          1..6130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     511..533
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     595..614
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      669..678
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2649..2693
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             2706..2723
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2835..2854)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2900..2955
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2954..2973)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3316..3332)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3316..3332)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3329..3351)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3340..3356
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3364..3370,3371..3388,3389..3394))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3409..3430
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3559..3576)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3730..4318)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3810..3829)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4489..5280,5281..5349))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5112..5131
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5350..5454)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5485..5864
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg
       61 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg
      121 gggggggggg gggggggggg gggggggggg gggggggsgg gggggggsgg ggccgcncts
      181 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
      241 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
      301 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
      361 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
      421 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg
      481 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
      541 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
      601 tgctggttat tgtgctgtct catcattttg gcaaagaatt gcggccgtct caggccgaat
      661 tcaagcttgc caccatgggg atccttccca gccctgggat gcctgcgctg ctctccctcg
      721 tgagccttct ctccgtgctg ctgatgggtt gcgtagctga aaccggtggt cctccaacgc
      781 tggctgtcca ggccaagtgt gatctctgct tgtccagtcc gtgccagaac cagggcacct
      841 gccacaacga cccccttgag gtgtacaggt gcgcctgccc cagcggctat aagggtcgag
      901 actgtgaggt gtccctggac agctgttcca gtggcccctg tgaaaatggg ggcacctgcc
      961 atgcacagga gggcgaggat gccccgttca cgtgctcctg tcccaccggc tttgaaggac
     1021 caacctgtgg ggtgaacaca gatgactgtg tggatcatgc ctgtgccaat gggggcgtct
     1081 gtgtggatgg tgtgggcaac tacacctgcc agtgccccct gcagtatgag ggaaaggcct
     1141 gtgagcagct ggtggacttg tgctctccgg atctgaaccc atgtcaacac gaggcccagt
     1201 gtgtgggcac cccggatggg cccaggtgtg agtgcatgcc aggttatgca ggtgacaact
     1261 gcagtgagaa ccaggatgac tgcagggacc accgctgcca gaatggggcc cagtgtatgg
     1321 atgaagtcaa cagctactcc tgcctctgtg ctgagggcta cagtggacag ctctgtgaga
     1381 tccctcccca tctgcctgcc cccaagagcc cctgtgaggg gactgagtgc cagaatgggg
     1441 ccaactgtgt ggaccagggc aacaggcctg tgtgccagtg cctcccaggc ttcggtggcc
     1501 ctgagtgtga gaagttgctc agtgtcaact ttgtggatcg ggacacttac ctgcagttca
     1561 ctgacctgca aaactggcca cgggccaaca tcacgttgca ggtctccacg gcagaggaca
     1621 atgggatcct tctgtacaac ggggacaacg accacattgc agttgagctg taccagggcc
     1681 atgtgcgtgt cagctacgac ccaggcagct accccagctc tgccatctac agtgctgaga
     1741 cgatcaacga tgggcaattc cacaccgttg agctggttgc ctttgaccag atggtgaatc
     1801 tctccattga tggcgggagc cccatgacca tggacaactt tggcaaacat tacacgctca
     1861 acagcgaggc gccactctat gtgggaggga tgcccgtgga tgtcaactca gctgccttcc
     1921 gcctgtggca gatcctcaac ggcaccggct tccacggttg catccgaaac ctgtacatca
     1981 acaacgagct gcaggacttc accaagacgc agatgaagcc aggcgtggtg ccaggctgcg
     2041 aaccctgccg caagctctac tgcctgcatg gcatctgcca gcccaatgcc accccagggc
     2101 ccatgtgcca ctgcgaggct ggctgggtgg gcctgcactg tgaccagccc gctgacggcc
     2161 cctgccatgg ccacaagtgt gtccatgggc aatgcgtgcc cctcgacgct ctttcctaca
     2221 gctgccagtg ccaggatggg tactcggggg cactgtgcaa ccaggccggg gccctggcag
     2281 agccctgcag aggcctgcag tgcctgcatg gccactgcca ggcctcaggc accaaggggg
     2341 cacactgtgt gtgtgacccc ggcttttcgg gcgagctgtg tgagcaagag tccgagtgcc
     2401 ggggggaccc tgtccgggac tttcaccagg tccagagggg ctatgccatc tgccagacca
     2461 cgcgccccct gtcatgggtg gagtgccggg gctcgtgccc aggccagggc tgctgccagg
     2521 gccttcggct gaagcggagg aagttcacct ttgagtgcag cgatgggacc tcttttgccg
     2581 aggaggtgga aaagcccacc aagtgtggct gtgccctctg cgcaggtacc ggaggttccg
     2641 gtggttccgg tctgaatgat atctttgaag ctcagaagat tgaatggcat gaaggacgta
     2701 ccaagcacca ccatcaccat cactaatgat cactcgagac tagtatcgcg ataattcact
     2761 cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac
     2821 aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt
     2881 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat
     2941 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat
     3001 gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt
     3061 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat
     3121 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct
     3181 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga
     3241 ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag
     3301 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     3361 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     3421 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     3481 gcggatcgat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3541 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     3601 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     3661 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     3721 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     3781 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     3841 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     3901 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     3961 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     4021 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     4081 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     4141 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     4201 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     4261 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     4321 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     4381 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     4441 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     4501 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     4561 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     4621 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     4681 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     4741 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     4801 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     4861 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     4921 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     4981 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     5041 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     5101 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     5161 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     5221 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     5281 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     5341 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     5401 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     5461 tccccgaaaa gtgccacctg ggtcgacatt gattattgac tagttattaa tagtaatcaa
     5521 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     5581 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     5641 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     5701 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     5761 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     5821 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
     5881 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
     5941 ttttttaatt attttgtgca gcgatggggg cggggggggg ggggggggss sssssngggg
     6001 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg ggggggnnng
     6061 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg
     6121 gggggggggg
//
