LOCUS       sequence-412476-        6533 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the genetically-encoded fluorescent dopamine (DA) control 
            sensor GRAB_rDA3mut in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Zhuo Y, Luo B, Yi X, Dong H, Miao X, Wan J, Williams JT, Campbell 
            MG, Cai R, Qian T, Li F, Weber SJ, Wang L, Li B, Wei Y, Li G, Wang 
            H, Zheng Y, Zhao Y, Wolf ME, Zhu Y, Watabe-Uchida M, Li Y
  TITLE     Improved green and red GRAB sensors for monitoring dopaminergic 
            activity in vivo.
  JOURNAL   Nat Methods. 2023 Nov 30. doi: 10.1038/s41592-023-02100-w.
  PUBMED    38036855
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 Nov 30. doi: 10.1038/s41592-023-02100-w."
COMMENT     Sequence Label: pAAV-hSyn-GRAB-rDA3mut
FEATURES             Location/Qualifiers
     source          1..6533
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   22..151
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     misc_feature    2681..3269
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2734..2754)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3152..3163)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3301..3777
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3500..3519)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3817..3957
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3817..3946
                     /label=AAV2 ITR
     rep_origin      4032..4487
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4119..4138)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4329..4350
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4504..4523)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4623..4645
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4683..4701)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4769..4873
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4874..4942,4943..5734)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5092..5111)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5905..6493
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6394..6413
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gctggccttt tgctcacatg tcctgcaggc agctgcgcgc tcgctcgctc actgaggccg
       61 cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag
      121 ggagtggcca actccatcac taggggttcc tgcggccgca cgcgtatgag gcggggtggg
      181 ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa
      241 attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca
      301 ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc
      361 cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgcaaac tccccttccc
      421 ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc
      481 gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct
      541 cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcagtc gagaaaccgg
      601 tgccaccatg aagacgatca tcgccctgag ctacatcttc tgcttggtgt tcgccgacta
      661 caaggacgat gatgacgcca tgaggactct gaacacctct gccatggacg ggactgggct
      721 ggtggtggag agggacttct ctgttcgtat cctcactgcc tgtttcctgt cgctgctcat
      781 cctgtccacg ctcctgggga acacgctggt ctgtgctgcc gttatcaggt tccgacacct
      841 gcggtccaag gtgaccaact tctttgtcat ctccttggct gtgtcagatc tcttggtggc
      901 cgtcctggtc atgccctgga aggcagtggc tgagattgct ggcttctggc cctttgggtc
      961 cttctgtaac atctgggtgg cctttgacat catgtgctcc actgcatcca tcctcaacct
     1021 ctgtgtgatc agcgtggaca ggtattgggc tatctccagc cctgagcggt atgagagaaa
     1081 gatgaccccc aaggcagcct tcatcctgat cagtgtggca tggaccttgt ctgtactcat
     1141 ctccttcatc ccagtgcagc tcagctggca caaggcaaaa cccacaagcc cctctgatgg
     1201 aaatgccact tccctggctg agaccataga caactgtgac tccagcctca gcaggacata
     1261 tgccatctca gcctctgtaa taccctttta catccctgtg gccatcatga ttgtctgcta
     1321 caccaggatc tacaggattg ctcagaaaaa gctctcctcc ttcatggttt ccgagcggat
     1381 gtaccccgag gacggcgccc tgaagagcga gatcaagaag gggctgaggc tgaaggacgg
     1441 cggccactac gccgccgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     1501 cggcgcctac atcgtggaca tcaagttgga catcgtgtcc cacaacgagg actacaccat
     1561 cgtggaacag tgcgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     1621 caagggaggt acaggcggga gtctggtgag caagggcgag gaggataaca tggccatcat
     1681 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     1741 gatcgagggc gagggcgagg gccgccccta cgaggccttt cagaccgcta agctgaaggt
     1801 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     1861 ctccaaggcc tacattaagc acccagccga catccccgac tacttcaagt cgtccttccc
     1921 cgagggcttc aggtgggagc gcgtgatgaa cttcgaggac ggcggcatta ttcacgtgaa
     1981 ccaggactcc tccctgcagg acggcgtatt catctacaag gtgaagctgc gcggcaccaa
     2041 cttccccccc gacggccccg taatgcagaa gaaaactatg gggtgggaac atcggaagga
     2101 cgaccttaaa agagaaacta aagtcctgaa gactctgtcg gtgatcatgg gtgtgtttgt
     2161 gtgctgttgg ctacctttct tcatcttgaa ctgcattttg cccttctgtg ggtctgggga
     2221 gacgcagccc ttctgcattg attccaacac ctttgacgtg tttgtgtggt ttgggtgggc
     2281 taattcatcc ttgaacccca tcatttatgc ctttaatgct gattttcgga aggcattttc
     2341 aaccctctta ggatgctaca gactttgccc tgcgacgaat aatgccatag agacggtgag
     2401 tatcaataac aatggggccg cgatgttttc cagccatcat gagccacgag gctccatctc
     2461 caaggagtgc aatctggttt acctgatccc acatgctgtg ggctcctctg aggacctgaa
     2521 aaaggaggag gcagctggca tcgccagacc cttggagaag ctgtccccag ccctatcggt
     2581 catattggac tatgacactg acgtctctct ggagaagatc caacccatca cacaaaacgg
     2641 tcagcaccca acctgagaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa
     2701 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     2761 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     2821 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     2881 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     2941 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     3001 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     3061 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctatgttg ccacctggat
     3121 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     3181 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     3241 tcggatctcc ctttgggccg cctccccgca tcgataccga gcgctgctcg agagatctac
     3301 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca
     3361 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
     3421 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
     3481 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg
     3541 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
     3601 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg
     3661 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct
     3721 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg
     3781 attttgtagg taaccacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
     3841 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     3901 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
     3961 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
     4021 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4081 cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc
     4141 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4201 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
     4261 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4321 ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc
     4381 ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta
     4441 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
     4501 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     4561 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     4621 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     4681 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     4741 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     4801 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     4861 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     4921 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     4981 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5041 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     5101 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     5161 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     5221 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     5281 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     5341 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     5401 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     5461 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     5521 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     5581 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     5641 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     5701 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     5761 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     5821 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     5881 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     5941 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6001 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     6061 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6121 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6181 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6241 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6301 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6361 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6421 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6481 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct ttt
//
