LOCUS       sequence-412477-       10527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting 
            murine MRE11 gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10527)
  AUTHORS   Cho MG, Kumar RJ, Lin CC, Boyer JA, Shahir JA, Fagan-Solis K, 
            Simpson DA, Fan C, Foster CE, Goddard AM, Lerner LM, Ellington SW, 
            Wang Q, Wang Y, Ho AY, Liu P, Perou CM, Zhang Q, McGinty RK, Purvis 
            JE, Gupta GP
  TITLE     MRE11 liberates cGAS from nucleosome sequestration during 
            tumorigenesis.
  JOURNAL   Nature. 2024 Jan 10. doi: 10.1038/s41586-023-06889-6.
  PUBMED    38200309
REFERENCE   2  (bases 1 to 10527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2024 Jan 10. doi: 10.1038/s41586-023-06889-6."
COMMENT     Sequence Label: Lenti-sgRNA-Cre-GpNLuc-sgmMRE11
FEATURES             Location/Qualifiers
     source          1..10527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(116..135)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      929..1517
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1418..1437
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1671..1688
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1805..1826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1841..1846,1847..1864,1865..1871)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1879..1895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1884..1906
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1903..1919
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1903..1919
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1968..2089
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1979..1998)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2033..2052
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2219..2593)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2612..2659)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(2707..3036)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2721..2856
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2775..2794)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     3021..3041
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(3050..3478)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3160..3181)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3372..3391
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3524..3748
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3737..3754
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             3780..4013
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4085..4673
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4191..4202
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4600..4620
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4689..5204)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             complement(join(5220..5930,5931..5933,5934..5936))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(5220..5930,5931..5933,5934..5936))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5262..5283)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5611..5630
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             complement(5937..5993)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(5994..7019)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLQ
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     CDS             complement(7020..7040)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(7066..7277)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_RNA        complement(7339..7414)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(7436..7684)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(7494..7513)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(7664..7684)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    7735..7852
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8150..8194)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8379..8612
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9105..9230
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             9277..9457
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(9475..9673)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9500..9520)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9675..10054
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     10226..10245
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        10320..10424
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10425..10493,10494..10527,1..758)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
       61 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
      121 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
      181 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
      241 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
      301 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
      361 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
      421 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
      481 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
      541 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
      601 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
      661 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
      721 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
      781 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
      841 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
      901 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
      961 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1021 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     1081 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1141 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     1201 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     1261 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     1321 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     1381 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     1441 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     1501 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     1561 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     1621 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     1681 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     1741 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     1801 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     1861 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     1921 atgattacgc caagctctag ctagaggtcg acggtataca gacatgataa gatacattga
     1981 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     2041 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagttg gggtgggcga
     2101 agaactccag catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga
     2161 ttccgaagcc caacctttca tagaaggcgg cggtggaatc gaaatctcgt agcacgtgtc
     2221 agtcctgctc ctcggccacg aagtgcacgc agttgccggc cgggtcgcgc agggcgaact
     2281 cccgccccca cggctgctcg ccgatctcgg tcatggccgg cccggaggcg tcccggaagt
     2341 tcgtggacac gacctccgac cactcggcgt acagctcgtc caggccgcgc acccacaccc
     2401 aggccagggt gttgtccggc accacctggt cctggaccgc gctgatgaac agggtcacgt
     2461 cgtcccggac cacaccggcg aagtcgtcct ccacgaagtc ccgggagaac ccgagccggt
     2521 cggtccagaa ctcgaccgct ccggcgacgt cgcgcgcggt gagcaccgga acggcactgg
     2581 tcaacttggc catggtttag ttcctcacct tgtcgtatta tactatgccg atatactatg
     2641 ccgatgatta attgtcaaca cgtgctgatc agatccgaaa atggatatac aagctcccgg
     2701 gagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
     2761 agaggcagag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
     2821 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
     2881 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
     2941 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
     3001 ggagcctggg gactttccac accctaactg acacacattc cacagaatta attcgcgtta
     3061 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     3121 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     3181 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     3241 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     3301 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     3361 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     3421 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtgg
     3481 ggataccccc tagagcccca gctggttctt tccgcctcag aagccataga gcccaccgca
     3541 tccccagcat gcctgctatt gtcttcccaa tcctccccct tgctgtcctg ccccacccca
     3601 ccccccagaa tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag
     3661 gaaaggacag tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca
     3721 acagatggct ggcaactaga aggcacagtc gaggctgatc agcgggttta aacgggccct
     3781 gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca
     3841 caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag
     3901 tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc
     3961 agtacaagca aaaagcagat cttgtcttcg ttgggagtga attagccctt ccagtccccc
     4021 cttttctttt aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagt
     4081 cgacgcgggg aggcggccca aagggagatc cgactcgtct gagggcgaag gcgaagacgc
     4141 ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa ggtccgctgg attgagggcc
     4201 gaagggacgt agcagaagga cgtcccgcgc agaatccagg tggcaacaca ggcgagcagc
     4261 caaggaaagg acgatgattt ccccgacaac accacggaat tgtcagtgcc caacagccga
     4321 gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt ccgccgtggc aatagggagg
     4381 gggaaagcga aagtcccgga aaggagctga caggtggtgg caatgcccca accagtgggg
     4441 gttgcgtcag caaacacagt gcacaccacg ccacgttgcc tgacaacggg ccacaactcc
     4501 tcataaagag acagcaacca ggatttatac aaggaggaga aaatgaaagc catacgggaa
     4561 gcaatagcat gatacaaagg cattaaagca gcgtatccac atagcgtaaa aggagcaaca
     4621 tagttaagaa taccagtcaa tctttcacaa attttgtaat ccagaggttg attgtcgact
     4681 taacgcgttt acgccagaat gcgttcgcac agccgccagc cggtcactcc gttgatggtt
     4741 actcggaaca gcagggagcc gtcggggttg atcaggcgct cgtcgataat tttgttgccg
     4801 ttccacaggg tccctgttac agtgatcttt ttgccgtcga acacggcgat gccttcatac
     4861 ggccgtccga aatagtcgat catgttcggc gtaaccccgt cgattaccag tgtgccatag
     4921 tgcaggatca ccttaaagtg atgatcatcc acagggtaca ccaccttaaa aattttttcg
     4981 atctggccca tttggtcgcc gctcagacct tcatacggga tgatgacatg gatgtcgatc
     5041 ttcagcccat tttcaccgct caggacaatc ctttggatcg gagttacgga caccccgaga
     5101 ttctgaaaca aactggacac acctccctgt tcaaggactt ggtccaggtt gtagccggct
     5161 gtctgtcgcc agtccccaac gaaatcttcg agtgtgaaga ccatacctcc ggagatatcc
     5221 ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc
     5281 atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actgggtgct caggtagtgg
     5341 ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg
     5401 agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc
     5461 ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc
     5521 cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg
     5581 gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag
     5641 atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc
     5701 atgtggtcgg ggtagcggct gaagcactgc acgccgtagg tcagggtggt cacgagggtg
     5761 ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag
     5821 gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc
     5881 agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct caccatcggt
     5941 ccaggattct cttcgacatc tccggcttgt ttcagcagag agaagtttgt tgcatcgcca
     6001 tcttccagca ggcgcaccat tgcccctgtt tcactatcca ggttacggat atagttcatg
     6061 acaatattta cattggtcca gccaccagct tgcatgatct ccggtattga aactccagcg
     6121 cgggccatat ctcgcgcggc tccgacacgg gcactgtgtc cagaccaggc caggtatctc
     6181 tgaccagagt catccttagc gccgtaaatc aatcgatgag ttgcttcaaa aatcccttcc
     6241 agggcgcgag ttgatagctg gctggtggca gatggcgcgg caacaccatt ttttctgacc
     6301 cggcaaaaca ggtagttatt cggatcatca gctacaccag atacggaaat ccatcgctcg
     6361 accagtttag ttacccccag gctaagtgcc ttctctacac ctgcggtgct aaccagcgtt
     6421 ttcgttctgc caatatggat taacattctc ccaccgtcag tacgtgagat atctttaacc
     6481 ctgatcctgg caatttcggc tatacgtaac agggtgttat aagcaatccc cagaaatgcc
     6541 agattacgta tatcctggca gcgatcgcta ttttccatga gtgaacgaac ctggtcgaaa
     6601 tcagtgcgtt cgaacgctag agcctgtttt gcacgttcac cggcatcaac gttttctttt
     6661 cggatccgcc gcataaccag tgaaacagca ttgctgtcac ttggtcgtgg cagcccggac
     6721 cgacgatgaa gcatgtttag ctggcccaaa tgttgctgga tagtttttac tgccagaccg
     6781 cgcgcctgaa gatatagaag ataatcgcga acatcttcag gttctgcggg aaaccatttc
     6841 cggttattca acttgcacca tgccgcccac gaccggcaaa cggactgaag cattttccag
     6901 gtatgctcag aaaacgcctg gcgatccctg aacatgtcca tcaggttctt gcgaacctca
     6961 tcactcgttg catcgaccgg taatgcaggc aaattttggt gtacggtcag taaattggac
     7021 accttcctct tcttcttggg catggtagat cctctagaac cggtcctgtg ttctggcggc
     7081 aaacccgttg cgaaaaagaa cgttcacggc gactactgca cttatatacg gttctccccc
     7141 accctcggga aaaaggcgga gccagtacac gacatcactt tcccagttta ccccgcgcca
     7201 ccttctctag gcaccggatc aattgccgac ccctcccccc aacttctcgg ggactgtggg
     7261 cgatgtgcgc tctgcccact gacgggcacc ggagccaatt cccactcctt tcaagaccta
     7321 gctagcgaat tcaaaaaagc accgactcgg tgccactttt tcaagttgat aacggactag
     7381 ccttatttta acttgctatt tctagctcta aaacgcctcg agtagtgatc tccacggtgt
     7441 ttcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa gttacggtaa
     7501 gcatatgata gtccatttta aaacataatt ttaaaactgc aaactaccca agaaattatt
     7561 actttctacg tcacgtattt tgtactaata tctttgtgtt tacagtcaaa ttaattctaa
     7621 ttatctctct aacagccttg tatcgtatat gcaaatatga aggaatcatg ggaaataggc
     7681 cctcggtacc ttaattaacc aaactggatc tctgctgtcc ctgtaataaa cccgaaaatt
     7741 ttgaattttt gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc
     7801 tactattctt tcccctgcac tgtacccccc aatcccccct tttcttttaa aattgtggat
     7861 gaatactgcc atttgtctgc agaattggcg cacgcagtgc cgatccgttc actaatcgaa
     7921 tggatctgtc tctgtctctc tctccacctt cttcttctat tccttcgggc ctgtcgggtc
     7981 ccctcggggt tgggaggtgg gtctgaaacg ataatggtga atatccctgc ctaactctat
     8041 tcactataga aagtacagca aaaactattc ttaaacctac caagcctcct actatcatta
     8101 tgaataattt tatataccac agccaatttg ttatgttaaa ccaattccac aaacttgccc
     8161 atttatctaa ttccaataat tcttgttcat tcttttcttg ctggttttgc gattcttcaa
     8221 ttaaggagtg tattaagctt gtgtaattgt taatttctct gtcccactcc atccaggtcg
     8281 tgtgattcca aatctgttcc agagatttat tactccaact agcattccaa ggcacagcag
     8341 tggtgcaaat gagttttcca gagcaacccc aaatccccag gagctgttga tcctttaggt
     8401 atctttccac agccaggatt cttgcctgga gctgcttgat gccccagact gtgagttgca
     8461 acagatgctg ttgcgcctca atagccctca gcaaattgtt ctgctgctgc actataccag
     8521 acaataattg tctggcctgt accgtcagcg tcattgacgc tgcgcccata gtgcttcctg
     8581 ctgctcccaa gaacccaagg aacaaagctc ctattcccac tgctcttttt tctctctgca
     8641 ccactcttct ctttgccttg gtgggtgcta ctcctaatgg ttcaattttt actactttat
     8701 atttatataa ttcacttctc caattgtccc tcatatctcc tcctccaggt ctgaagatca
     8761 gcggccgctt gctgtgcggt ggtcttactt ttgttttgct cttcctctat cttgtctaaa
     8821 gcttccttgg tgtcttttat ctctatcctt tgatgcacac aatagagggt tgctactgta
     8881 ttatataatg atctaagttc ttctgatcct gtctgaaggg atggttgtag ctgtcccagt
     8941 atttgtctac agccttctga tgtttctaac aggccaggat taactgcgaa tcgttctagc
     9001 tccctgcttg cccatactat atgttttaat ttatattttt tctttccccc tggccttaac
     9061 cgaatttttt cccatcgcga tctaattctc ccccgcttaa tactgacgct ctcgcaccca
     9121 tctctctcct tctagcctcc gctagtcaaa atttttggcg tactcaccag tcgccgcccc
     9181 tcgcctcttg ccgtgcgcgc ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt
     9241 ttccctttcg ctttcaagtc cctgttcggg cgccactgct agagattttc cacactgact
     9301 aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca cacactactt
     9361 gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt tagccagaga
     9421 gctcccaggc tcagatctgg tctaaccaga gagacccagt acaggcaaaa cgcgctgctt
     9481 atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta
     9541 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
     9601 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
     9661 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
     9721 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
     9781 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
     9841 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
     9901 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
     9961 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
    10021 gattactatt aataactagt caataatcaa tgtcaacgcg tatatctggc ccgtacatcg
    10081 cgaagcagcg caaaacgcct aaccctaagc agattcttca tgcaattgtc ggtcaagcct
    10141 tgccttgttg tagcttaaat tttgctcgcg cactactcag cgacctccaa cacacaagca
    10201 gggagcagat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
    10261 aggggatcgg gagatctccc gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc
    10321 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
    10381 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
    10441 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
    10501 aaacgctggt gaaagtaaaa gatgctg
//
