LOCUS       sequence-412501-       10527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting 
            murine ZBP1#1 gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10527)
  AUTHORS   Cho MG, Kumar RJ, Lin CC, Boyer JA, Shahir JA, Fagan-Solis K, 
            Simpson DA, Fan C, Foster CE, Goddard AM, Lerner LM, Ellington SW, 
            Wang Q, Wang Y, Ho AY, Liu P, Perou CM, Zhang Q, McGinty RK, Purvis 
            JE, Gupta GP
  TITLE     MRE11 liberates cGAS from nucleosome sequestration during 
            tumorigenesis.
  JOURNAL   Nature. 2024 Jan 10. doi: 10.1038/s41586-023-06889-6.
  PUBMED    38200309
REFERENCE   2  (bases 1 to 10527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2024 Jan 10. doi: 10.1038/s41586-023-06889-6."
COMMENT     Sequence Label: Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#1
FEATURES             Location/Qualifiers
     source          1..10527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(107..126)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      920..1508
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1409..1428
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1662..1679
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1796..1817
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1832..1837,1838..1855,1856..1862)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1870..1886
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1875..1897
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1894..1910
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1894..1910
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1959..2080
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1970..1989)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2024..2043
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2210..2584)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2603..2650)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(2698..3027)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2712..2847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2766..2785)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     3012..3032
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(3041..3469)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3151..3172)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3363..3382
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3515..3739
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3728..3745
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             3771..4004
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4076..4664
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4182..4193
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4591..4611
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4680..5195)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             complement(join(5211..5921,5922..5924,5925..5927))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(5211..5921,5922..5924,5925..5927))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5253..5274)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5602..5621
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             complement(5928..5984)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(5985..7010)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLQ
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     CDS             complement(7011..7031)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(7057..7268)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_RNA        complement(7330..7405)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(7427..7675)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(7485..7504)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(7655..7675)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    7726..7843
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(8141..8185)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8370..8603
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9096..9221
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             9268..9448
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(9466..9664)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9491..9511)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9666..10045
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     10217..10236
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        10311..10415
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10416..10484,10485..10527,1..749)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
       61 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      121 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      181 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      241 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      301 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      361 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      421 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      481 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      541 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      601 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      661 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      721 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
      781 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
      841 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
      901 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      961 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1021 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1081 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1141 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1201 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1261 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1321 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1381 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1441 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1501 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1561 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1621 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1681 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1741 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     1801 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     1861 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     1921 ccaagctcta gctagaggtc gacggtatac agacatgata agatacattg atgagtttgg
     1981 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     2041 tgctttattt gtaaccatta taagctgcaa taaacaagtt ggggtgggcg aagaactcca
     2101 gcatgagatc cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc
     2161 ccaacctttc atagaaggcg gcggtggaat cgaaatctcg tagcacgtgt cagtcctgct
     2221 cctcggccac gaagtgcacg cagttgccgg ccgggtcgcg cagggcgaac tcccgccccc
     2281 acggctgctc gccgatctcg gtcatggccg gcccggaggc gtcccggaag ttcgtggaca
     2341 cgacctccga ccactcggcg tacagctcgt ccaggccgcg cacccacacc caggccaggg
     2401 tgttgtccgg caccacctgg tcctggaccg cgctgatgaa cagggtcacg tcgtcccgga
     2461 ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa cccgagccgg tcggtccaga
     2521 actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg aacggcactg gtcaacttgg
     2581 ccatggttta gttcctcacc ttgtcgtatt atactatgcc gatatactat gccgatgatt
     2641 aattgtcaac acgtgctgat cagatccgaa aatggatata caagctcccg ggagcttttt
     2701 gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggcaga
     2761 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     2821 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
     2881 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     2941 cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     3001 ggactttcca caccctaact gacacacatt ccacagaatt aattcgcgtt aaatttttgt
     3061 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     3121 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     3181 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     3241 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     3301 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     3361 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     3421 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtg gggatacccc
     3481 ctagagcccc agctggttct ttccgcctca gaagccatag agcccaccgc atccccagca
     3541 tgcctgctat tgtcttccca atcctccccc ttgctgtcct gccccacccc accccccaga
     3601 atagaatgac acctactcag acaatgcgat gcaatttcct cattttatta ggaaaggaca
     3661 gtgggagtgg caccttccag ggtcaaggaa ggcacggggg aggggcaaac aacagatggc
     3721 tggcaactag aaggcacagt cgaggctgat cagcgggttt aaacgggccc tgctagagat
     3781 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
     3841 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
     3901 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacaagc
     3961 aaaaagcaga tcttgtcttc gttgggagtg aattagccct tccagtcccc ccttttcttt
     4021 taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag tcgacgcggg
     4081 gaggcggccc aaagggagat ccgactcgtc tgagggcgaa ggcgaagacg cggaagaggc
     4141 cgcagagccg gcagcaggcc gcgggaagga aggtccgctg gattgagggc cgaagggacg
     4201 tagcagaagg acgtcccgcg cagaatccag gtggcaacac aggcgagcag ccaaggaaag
     4261 gacgatgatt tccccgacaa caccacggaa ttgtcagtgc ccaacagccg agcccctgtc
     4321 cagcagcggg caaggcaggc ggcgatgagt tccgccgtgg caatagggag ggggaaagcg
     4381 aaagtcccgg aaaggagctg acaggtggtg gcaatgcccc aaccagtggg ggttgcgtca
     4441 gcaaacacag tgcacaccac gccacgttgc ctgacaacgg gccacaactc ctcataaaga
     4501 gacagcaacc aggatttata caaggaggag aaaatgaaag ccatacggga agcaatagca
     4561 tgatacaaag gcattaaagc agcgtatcca catagcgtaa aaggagcaac atagttaaga
     4621 ataccagtca atctttcaca aattttgtaa tccagaggtt gattgtcgac ttaacgcgtt
     4681 tacgccagaa tgcgttcgca cagccgccag ccggtcactc cgttgatggt tactcggaac
     4741 agcagggagc cgtcggggtt gatcaggcgc tcgtcgataa ttttgttgcc gttccacagg
     4801 gtccctgtta cagtgatctt tttgccgtcg aacacggcga tgccttcata cggccgtccg
     4861 aaatagtcga tcatgttcgg cgtaaccccg tcgattacca gtgtgccata gtgcaggatc
     4921 accttaaagt gatgatcatc cacagggtac accaccttaa aaattttttc gatctggccc
     4981 atttggtcgc cgctcagacc ttcatacggg atgatgacat ggatgtcgat cttcagccca
     5041 ttttcaccgc tcaggacaat cctttggatc ggagttacgg acaccccgag attctgaaac
     5101 aaactggaca cacctccctg ttcaaggact tggtccaggt tgtagccggc tgtctgtcgc
     5161 cagtccccaa cgaaatcttc gagtgtgaag accatacctc cggagatatc cttgtacagc
     5221 tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg
     5281 cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc
     5341 agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg
     5401 ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg
     5461 tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg
     5521 ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc
     5581 tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc
     5641 tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg
     5701 gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc
     5761 acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg
     5821 ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc
     5881 aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatcgg tccaggattc
     5941 tcttcgacat ctccggcttg tttcagcaga gagaagtttg ttgcatcgcc atcttccagc
     6001 aggcgcacca ttgcccctgt ttcactatcc aggttacgga tatagttcat gacaatattt
     6061 acattggtcc agccaccagc ttgcatgatc tccggtattg aaactccagc gcgggccata
     6121 tctcgcgcgg ctccgacacg ggcactgtgt ccagaccagg ccaggtatct ctgaccagag
     6181 tcatccttag cgccgtaaat caatcgatga gttgcttcaa aaatcccttc cagggcgcga
     6241 gttgatagct ggctggtggc agatggcgcg gcaacaccat tttttctgac ccggcaaaac
     6301 aggtagttat tcggatcatc agctacacca gatacggaaa tccatcgctc gaccagttta
     6361 gttaccccca ggctaagtgc cttctctaca cctgcggtgc taaccagcgt tttcgttctg
     6421 ccaatatgga ttaacattct cccaccgtca gtacgtgaga tatctttaac cctgatcctg
     6481 gcaatttcgg ctatacgtaa cagggtgtta taagcaatcc ccagaaatgc cagattacgt
     6541 atatcctggc agcgatcgct attttccatg agtgaacgaa cctggtcgaa atcagtgcgt
     6601 tcgaacgcta gagcctgttt tgcacgttca ccggcatcaa cgttttcttt tcggatccgc
     6661 cgcataacca gtgaaacagc attgctgtca cttggtcgtg gcagcccgga ccgacgatga
     6721 agcatgttta gctggcccaa atgttgctgg atagttttta ctgccagacc gcgcgcctga
     6781 agatatagaa gataatcgcg aacatcttca ggttctgcgg gaaaccattt ccggttattc
     6841 aacttgcacc atgccgccca cgaccggcaa acggactgaa gcattttcca ggtatgctca
     6901 gaaaacgcct ggcgatccct gaacatgtcc atcaggttct tgcgaacctc atcactcgtt
     6961 gcatcgaccg gtaatgcagg caaattttgg tgtacggtca gtaaattgga caccttcctc
     7021 ttcttcttgg gcatggtaga tcctctagaa ccggtcctgt gttctggcgg caaacccgtt
     7081 gcgaaaaaga acgttcacgg cgactactgc acttatatac ggttctcccc caccctcggg
     7141 aaaaaggcgg agccagtaca cgacatcact ttcccagttt accccgcgcc accttctcta
     7201 ggcaccggat caattgccga cccctccccc caacttctcg gggactgtgg gcgatgtgcg
     7261 ctctgcccac tgacgggcac cggagccaat tcccactcct ttcaagacct agctagcgaa
     7321 ttcaaaaaag caccgactcg gtgccacttt ttcaagttga taacggacta gccttatttt
     7381 aacttgctat ttctagctct aaaacccgtc atcgctcaac acctgcggtg tttcgtcctt
     7441 tccacaagat atataaagcc aagaaatcga aatactttca agttacggta agcatatgat
     7501 agtccatttt aaaacataat tttaaaactg caaactaccc aagaaattat tactttctac
     7561 gtcacgtatt ttgtactaat atctttgtgt ttacagtcaa attaattcta attatctctc
     7621 taacagcctt gtatcgtata tgcaaatatg aaggaatcat gggaaatagg ccctcggtac
     7681 cttaattaac caaactggat ctctgctgtc cctgtaataa acccgaaaat tttgaatttt
     7741 tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt gctattatgt ctactattct
     7801 ttcccctgca ctgtaccccc caatcccccc ttttctttta aaattgtgga tgaatactgc
     7861 catttgtctg cagaattggc gcacgcagtg ccgatccgtt cactaatcga atggatctgt
     7921 ctctgtctct ctctccacct tcttcttcta ttccttcggg cctgtcgggt cccctcgggg
     7981 ttgggaggtg ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag
     8041 aaagtacagc aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt
     8101 ttatatacca cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta
     8161 attccaataa ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt
     8221 gtattaagct tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc
     8281 aaatctgttc cagagattta ttactccaac tagcattcca aggcacagca gtggtgcaaa
     8341 tgagttttcc agagcaaccc caaatcccca ggagctgttg atcctttagg tatctttcca
     8401 cagccaggat tcttgcctgg agctgcttga tgccccagac tgtgagttgc aacagatgct
     8461 gttgcgcctc aatagccctc agcaaattgt tctgctgctg cactatacca gacaataatt
     8521 gtctggcctg taccgtcagc gtcattgacg ctgcgcccat agtgcttcct gctgctccca
     8581 agaacccaag gaacaaagct cctattccca ctgctctttt ttctctctgc accactcttc
     8641 tctttgcctt ggtgggtgct actcctaatg gttcaatttt tactacttta tatttatata
     8701 attcacttct ccaattgtcc ctcatatctc ctcctccagg tctgaagatc agcggccgct
     8761 tgctgtgcgg tggtcttact tttgttttgc tcttcctcta tcttgtctaa agcttccttg
     8821 gtgtctttta tctctatcct ttgatgcaca caatagaggg ttgctactgt attatataat
     8881 gatctaagtt cttctgatcc tgtctgaagg gatggttgta gctgtcccag tatttgtcta
     8941 cagccttctg atgtttctaa caggccagga ttaactgcga atcgttctag ctccctgctt
     9001 gcccatacta tatgttttaa tttatatttt ttctttcccc ctggccttaa ccgaattttt
     9061 tcccatcgcg atctaattct cccccgctta atactgacgc tctcgcaccc atctctctcc
     9121 ttctagcctc cgctagtcaa aatttttggc gtactcacca gtcgccgccc ctcgcctctt
     9181 gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg tttccctttc
     9241 gctttcaagt ccctgttcgg gcgccactgc tagagatttt ccacactgac taaaagggtc
     9301 tgagggatct ctagttacca gagtcacaca acagacgggc acacactact tgaagcactc
     9361 aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag agctcccagg
     9421 ctcagatctg gtctaaccag agagacccag tacaggcaaa acgcgctgct tatatagacc
     9481 tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt
     9541 ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga
     9601 cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc
     9661 atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat
     9721 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
     9781 ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac
     9841 tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt
     9901 attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt
     9961 tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat
    10021 taataactag tcaataatca atgtcaacgc gtatatctgg cccgtacatc gcgaagcagc
    10081 gcaaaacgcc taaccctaag cagattcttc atgcaattgt cggtcaagcc ttgccttgtt
    10141 gtagcttaaa ttttgctcgc gcactactca gcgacctcca acacacaagc agggagcaga
    10201 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca taggggatcg
    10261 ggagatctcc cgatccgtcg acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
    10321 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
    10381 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
    10441 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
    10501 tgaaagtaaa agatgctgaa gatcagt
//
