LOCUS       sequence-412506-       10527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This sgControl, located in the TP53BP1 intron, serves as a control 
            sgRNA for the others. The vector was cloned from 
            Lenti-sgRNA-Cre-GpNLuc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10527)
  AUTHORS   Cho MG, Kumar RJ, Lin CC, Boyer JA, Shahir JA, Fagan-Solis K, 
            Simpson DA, Fan C, Foster CE, Goddard AM, Lerner LM, Ellington SW, 
            Wang Q, Wang Y, Ho AY, Liu P, Perou CM, Zhang Q, McGinty RK, Purvis 
            JE, Gupta GP
  TITLE     MRE11 liberates cGAS from nucleosome sequestration during 
            tumorigenesis.
  JOURNAL   Nature. 2024 Jan 10. doi: 10.1038/s41586-023-06889-6.
  PUBMED    38200309
REFERENCE   2  (bases 1 to 10527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2024 Jan 10. doi: 10.1038/s41586-023-06889-6."
COMMENT     Sequence Label: Lenti-sgRNA-Cre-GpNluc-sgControl
FEATURES             Location/Qualifiers
     source          1..10527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(94..141)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(189..518)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      203..338
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(257..276)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     503..523
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(532..960)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(642..663)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     854..873
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    1006..1230
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1219..1236
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             1262..1495
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    1567..2155
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1673..1684
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2082..2102
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2171..2686)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             complement(join(2702..3412,3413..3415,3416..3418))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2702..3412,3413..3415,3416..3418))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2744..2765)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3093..3112
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             complement(3419..3475)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(3476..4501)
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLQ
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     CDS             complement(4502..4522)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(4548..4759)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_RNA        complement(4821..4896)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(4918..5166)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(4976..4995)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(5146..5166)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    5217..5334
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(5632..5676)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5861..6094
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6587..6712
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6759..6939
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6957..7155)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6982..7002)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7157..7536
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     7708..7727
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        7802..7906
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7907..7975,7976..8767)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8125..8144)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8938..9526
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9427..9446
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9680..9697
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9814..9835
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9850..9855,9856..9873,9874..9880)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9888..9904
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9893..9915
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9912..9928
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9912..9928
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    9977..10098
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9988..10007)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10042..10061
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(10228..10527,1..75))
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 gtcggtccag aactcgaccg ctccggcgac gtcgcgcgcg gtgagcaccg gaacggcact
       61 ggtcaacttg gccatggttt agttcctcac cttgtcgtat tatactatgc cgatatacta
      121 tgccgatgat taattgtcaa cacgtgctga tcagatccga aaatggatat acaagctccc
      181 gggagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc
      241 tcagaggcag aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc
      301 ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact
      361 atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
      421 gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
      481 ggggagcctg gggactttcc acaccctaac tgacacacat tccacagaat taattcgcgt
      541 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
      601 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
      661 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
      721 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
      781 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
      841 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
      901 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
      961 ggggataccc cctagagccc cagctggttc tttccgcctc agaagccata gagcccaccg
     1021 catccccagc atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc
     1081 caccccccag aatagaatga cacctactca gacaatgcga tgcaatttcc tcattttatt
     1141 aggaaaggac agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa
     1201 caacagatgg ctggcaacta gaaggcacag tcgaggctga tcagcgggtt taaacgggcc
     1261 ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca
     1321 cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc
     1381 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
     1441 ccagtacaag caaaaagcag atcttgtctt cgttgggagt gaattagccc ttccagtccc
     1501 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
     1561 gtcgacgcgg ggaggcggcc caaagggaga tccgactcgt ctgagggcga aggcgaagac
     1621 gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg aaggtccgct ggattgaggg
     1681 ccgaagggac gtagcagaag gacgtcccgc gcagaatcca ggtggcaaca caggcgagca
     1741 gccaaggaaa ggacgatgat ttccccgaca acaccacgga attgtcagtg cccaacagcc
     1801 gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag ttccgccgtg gcaataggga
     1861 gggggaaagc gaaagtcccg gaaaggagct gacaggtggt ggcaatgccc caaccagtgg
     1921 gggttgcgtc agcaaacaca gtgcacacca cgccacgttg cctgacaacg ggccacaact
     1981 cctcataaag agacagcaac caggatttat acaaggagga gaaaatgaaa gccatacggg
     2041 aagcaatagc atgatacaaa ggcattaaag cagcgtatcc acatagcgta aaaggagcaa
     2101 catagttaag aataccagtc aatctttcac aaattttgta atccagaggt tgattgtcga
     2161 cttaacgcgt ttacgccaga atgcgttcgc acagccgcca gccggtcact ccgttgatgg
     2221 ttactcggaa cagcagggag ccgtcggggt tgatcaggcg ctcgtcgata attttgttgc
     2281 cgttccacag ggtccctgtt acagtgatct ttttgccgtc gaacacggcg atgccttcat
     2341 acggccgtcc gaaatagtcg atcatgttcg gcgtaacccc gtcgattacc agtgtgccat
     2401 agtgcaggat caccttaaag tgatgatcat ccacagggta caccacctta aaaatttttt
     2461 cgatctggcc catttggtcg ccgctcagac cttcatacgg gatgatgaca tggatgtcga
     2521 tcttcagccc attttcaccg ctcaggacaa tcctttggat cggagttacg gacaccccga
     2581 gattctgaaa caaactggac acacctccct gttcaaggac ttggtccagg ttgtagccgg
     2641 ctgtctgtcg ccagtcccca acgaaatctt cgagtgtgaa gaccatacct ccggagatat
     2701 ccttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga
     2761 ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt
     2821 ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg
     2881 cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt
     2941 tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt
     3001 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
     3061 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga
     3121 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct
     3181 tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg
     3241 tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt
     3301 aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt
     3361 ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatcg
     3421 gtccaggatt ctcttcgaca tctccggctt gtttcagcag agagaagttt gttgcatcgc
     3481 catcttccag caggcgcacc attgcccctg tttcactatc caggttacgg atatagttca
     3541 tgacaatatt tacattggtc cagccaccag cttgcatgat ctccggtatt gaaactccag
     3601 cgcgggccat atctcgcgcg gctccgacac gggcactgtg tccagaccag gccaggtatc
     3661 tctgaccaga gtcatcctta gcgccgtaaa tcaatcgatg agttgcttca aaaatccctt
     3721 ccagggcgcg agttgatagc tggctggtgg cagatggcgc ggcaacacca ttttttctga
     3781 cccggcaaaa caggtagtta ttcggatcat cagctacacc agatacggaa atccatcgct
     3841 cgaccagttt agttaccccc aggctaagtg ccttctctac acctgcggtg ctaaccagcg
     3901 ttttcgttct gccaatatgg attaacattc tcccaccgtc agtacgtgag atatctttaa
     3961 ccctgatcct ggcaatttcg gctatacgta acagggtgtt ataagcaatc cccagaaatg
     4021 ccagattacg tatatcctgg cagcgatcgc tattttccat gagtgaacga acctggtcga
     4081 aatcagtgcg ttcgaacgct agagcctgtt ttgcacgttc accggcatca acgttttctt
     4141 ttcggatccg ccgcataacc agtgaaacag cattgctgtc acttggtcgt ggcagcccgg
     4201 accgacgatg aagcatgttt agctggccca aatgttgctg gatagttttt actgccagac
     4261 cgcgcgcctg aagatataga agataatcgc gaacatcttc aggttctgcg ggaaaccatt
     4321 tccggttatt caacttgcac catgccgccc acgaccggca aacggactga agcattttcc
     4381 aggtatgctc agaaaacgcc tggcgatccc tgaacatgtc catcaggttc ttgcgaacct
     4441 catcactcgt tgcatcgacc ggtaatgcag gcaaattttg gtgtacggtc agtaaattgg
     4501 acaccttcct cttcttcttg ggcatggtag atcctctaga accggtcctg tgttctggcg
     4561 gcaaacccgt tgcgaaaaag aacgttcacg gcgactactg cacttatata cggttctccc
     4621 ccaccctcgg gaaaaaggcg gagccagtac acgacatcac tttcccagtt taccccgcgc
     4681 caccttctct aggcaccgga tcaattgccg acccctcccc ccaacttctc ggggactgtg
     4741 ggcgatgtgc gctctgccca ctgacgggca ccggagccaa ttcccactcc tttcaagacc
     4801 tagctagcga attcaaaaaa gcaccgactc ggtgccactt tttcaagttg ataacggact
     4861 agccttattt taacttgcta tttctagctc taaaacctga tttgaataat gatgcccggt
     4921 gtttcgtcct ttccacaaga tatataaagc caagaaatcg aaatactttc aagttacggt
     4981 aagcatatga tagtccattt taaaacataa ttttaaaact gcaaactacc caagaaatta
     5041 ttactttcta cgtcacgtat tttgtactaa tatctttgtg tttacagtca aattaattct
     5101 aattatctct ctaacagcct tgtatcgtat atgcaaatat gaaggaatca tgggaaatag
     5161 gccctcggta ccttaattaa ccaaactgga tctctgctgt ccctgtaata aacccgaaaa
     5221 ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg
     5281 tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt aaaattgtgg
     5341 atgaatactg ccatttgtct gcagaattgg cgcacgcagt gccgatccgt tcactaatcg
     5401 aatggatctg tctctgtctc tctctccacc ttcttcttct attccttcgg gcctgtcggg
     5461 tcccctcggg gttgggaggt gggtctgaaa cgataatggt gaatatccct gcctaactct
     5521 attcactata gaaagtacag caaaaactat tcttaaacct accaagcctc ctactatcat
     5581 tatgaataat tttatatacc acagccaatt tgttatgtta aaccaattcc acaaacttgc
     5641 ccatttatct aattccaata attcttgttc attcttttct tgctggtttt gcgattcttc
     5701 aattaaggag tgtattaagc ttgtgtaatt gttaatttct ctgtcccact ccatccaggt
     5761 cgtgtgattc caaatctgtt ccagagattt attactccaa ctagcattcc aaggcacagc
     5821 agtggtgcaa atgagttttc cagagcaacc ccaaatcccc aggagctgtt gatcctttag
     5881 gtatctttcc acagccagga ttcttgcctg gagctgcttg atgccccaga ctgtgagttg
     5941 caacagatgc tgttgcgcct caatagccct cagcaaattg ttctgctgct gcactatacc
     6001 agacaataat tgtctggcct gtaccgtcag cgtcattgac gctgcgccca tagtgcttcc
     6061 tgctgctccc aagaacccaa ggaacaaagc tcctattccc actgctcttt tttctctctg
     6121 caccactctt ctctttgcct tggtgggtgc tactcctaat ggttcaattt ttactacttt
     6181 atatttatat aattcacttc tccaattgtc cctcatatct cctcctccag gtctgaagat
     6241 cagcggccgc ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct atcttgtcta
     6301 aagcttcctt ggtgtctttt atctctatcc tttgatgcac acaatagagg gttgctactg
     6361 tattatataa tgatctaagt tcttctgatc ctgtctgaag ggatggttgt agctgtccca
     6421 gtatttgtct acagccttct gatgtttcta acaggccagg attaactgcg aatcgttcta
     6481 gctccctgct tgcccatact atatgtttta atttatattt tttctttccc cctggcctta
     6541 accgaatttt ttcccatcgc gatctaattc tcccccgctt aatactgacg ctctcgcacc
     6601 catctctctc cttctagcct ccgctagtca aaatttttgg cgtactcacc agtcgccgcc
     6661 cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag agctcctctg
     6721 gtttcccttt cgctttcaag tccctgttcg ggcgccactg ctagagattt tccacactga
     6781 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     6841 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     6901 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaggcaa aacgcgctgc
     6961 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt
     7021 tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa
     7081 tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact
     7141 gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag
     7201 gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga
     7261 cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt
     7321 accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa
     7381 catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat
     7441 ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa
     7501 ttgattacta ttaataacta gtcaataatc aatgtcaacg cgtatatctg gcccgtacat
     7561 cgcgaagcag cgcaaaacgc ctaaccctaa gcagattctt catgcaattg tcggtcaagc
     7621 cttgccttgt tgtagcttaa attttgctcg cgcactactc agcgacctcc aacacacaag
     7681 cagggagcag atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     7741 ataggggatc gggagatctc ccgatccgtc gacgtcaggt ggcacttttc ggggaaatgt
     7801 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7861 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7921 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7981 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     8041 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     8101 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     8161 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     8221 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     8281 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     8341 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     8401 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     8461 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     8521 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8581 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8641 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8701 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8761 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8821 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8881 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8941 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     9001 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     9061 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     9121 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     9181 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     9241 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     9301 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     9361 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     9421 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     9481 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     9541 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     9601 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     9661 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     9721 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     9781 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     9841 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     9901 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctctagc tagaggtcga
     9961 cggtatacag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
    10021 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
    10081 agctgcaata aacaagttgg ggtgggcgaa gaactccagc atgagatccc cgcgctggag
    10141 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc
    10201 ggtggaatcg aaatctcgta gcacgtgtca gtcctgctcc tcggccacga agtgcacgca
    10261 gttgccggcc gggtcgcgca gggcgaactc ccgcccccac ggctgctcgc cgatctcggt
    10321 catggccggc ccggaggcgt cccggaagtt cgtggacacg acctccgacc actcggcgta
    10381 cagctcgtcc aggccgcgca cccacaccca ggccagggtg ttgtccggca ccacctggtc
    10441 ctggaccgcg ctgatgaaca gggtcacgtc gtcccggacc acaccggcga agtcgtcctc
    10501 cacgaagtcc cgggagaacc cgagccg
//
