LOCUS       sequence-41253-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Garcia DM, Baek D, Shin C, Bell GW, Grimson A, Bartel DP
  TITLE     Weak seed-pairing stability and high target-site abundance decrease 
            the proficiency of lsy-6 and other microRNAs.
  JOURNAL   Nat Struct Mol Biol. 2011 Sep 11;18(10):1139-46. doi: 
            10.1038/nsmb.2115.
  PUBMED    21909094
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb"; 
            journalName: "Nat Struct Mol Biol"; date: "2011-09-11- 11"; volume: 
            "18"; issue: "10"; pages: "1139-46"
COMMENT     Sequence Label: pAG249
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    563..684
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(574..593)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     628..647
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ttcctaatct gaatttgtca aagtaaaagg tcttcatttt tcgcaagaag atgcacctga
       61 tgaaatggga aaatatatca aatcgttcgt tgagcgagtt ctcaaaaatg aacaataatt
      121 ctaggagctc catgctttta ggacagaatg gagacttata cacccaaagt ttatctgtgt
      181 ttgtttgtaa gtattatgat gctaaaaatt tagatttatt ctaaatgtat ttgatgtgaa
      241 ttaaaataaa tattttttca tgtgaaattt attttggttc ctaaaatgga agcctaccac
      301 attgcattgt aatacagtgt attatgttca gtgtctaaaa actgctaatt aagtcataat
      361 ttaagatgct atgtatctgt tatttaaaac atggagaaac agggccttta ttccattcat
      421 attcataaga gcatatttat cctgcattga aaatgcatta cttttgcaca ttgatattaa
      481 ctgttgtcca acaaataagt atcggagtac gtgagaatat tcccactagt tgttctagag
      541 cggccgcttc gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga
      601 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
      661 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
      721 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc
      781 gataaggatc caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      841 ttctaataca ttcaatatgt atccgctc
//
