LOCUS       sequence-41254-e         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Garcia DM, Baek D, Shin C, Bell GW, Grimson A, Bartel DP
  TITLE     Weak seed-pairing stability and high target-site abundance decrease 
            the proficiency of lsy-6 and other microRNAs.
  JOURNAL   Nat Struct Mol Biol. 2011 Sep 11;18(10):1139-46. doi: 
            10.1038/nsmb.2115.
  PUBMED    21909094
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb"; 
            journalName: "Nat Struct Mol Biol"; date: "2011-09-11- 11"; volume: 
            "18"; issue: "10"; pages: "1139-46"
COMMENT     Sequence Label: pCS249
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    552..673
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(563..582)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     617..636
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gaatttgtca agtaaaaggt cttcattttt cgcaagaaga tgcacctgat gaaatgggaa
       61 aatatatcaa atcgttcgtt gagcgagttc tcaaaaatga acaataattc taggagctcc
      121 atgcttttag gacagaatgg agacttatac acccaaagtt tatctgtgtt tgtttgtaag
      181 tattatgatg ctaaaaattt agatttattc taaatgtatt tgacgcgaat taaaataaat
      241 attttttcac gcgaaattta ttttggttcc taaaatggaa gcctaccaca ttgcattgta
      301 atacagtgta ttatgttcag tgtctaaaaa ctgctaatta agtcataatt taagatgcta
      361 tgtatctgtt atttaaaaca tggagaaaca gggcctttat tccattcata ttcataagag
      421 catatttatc ctgcattgaa aatgcattac ttttgcacat tgatattaac tgttgtccaa
      481 caaataagta tcggagtacg tgagaatatt cccactagtt gttctagagc ggccgcttcg
      541 agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
      601 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
      661 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
      721 gtgggaggtt ttttaaagca agtaaaacct ctac
//
