LOCUS       sequence-412552-        9144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsSmg7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9144)
  AUTHORS   Loh B, Jonas S, Izaurralde E
  TITLE     The SMG5-SMG7 heterodimer directly recruits the CCR4-NOT deadenylase
            complex to mRNAs containing nonsense codons via interaction with 
            POP2.
  JOURNAL   Genes Dev. 2013 Oct 1;27(19):2125-38. doi: 10.1101/gad.226951.113.
  PUBMED    24115769
REFERENCE   2  (bases 1 to 9144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.226951"; journalName: "Genes Dev"; date: "2013-10-1- 
            1"; volume: "27"; issue: "19"; pages: "2125-38"
COMMENT     Sequence Label: pcDNA3.1-MS2-HA-HsSmg7_T
FEATURES             Location/Qualifiers
     source          1..9144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      661..670
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1099..1125
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(4463..4481)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4464..4481)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4501..4518)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4507..4731
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4777..5205
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4864..4883)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5074..5095
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5214..5234)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5219..5548
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5399..5534
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5461..5480
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5615..6409
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5669..5688)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6279..6298
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6583..6704
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6620..6639)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6674..6693
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6753..6769)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6753..6769)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6766..6788)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6777..6793
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6801..6807,6808..6825,6826..6831))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6846..6867
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6984..7001)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7155..7743)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7235..7254)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7914..8705,8706..8774))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8537..8556
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8775..8879)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8954..8973)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt
      661 gccaccatgg cttctaactt tactcagttc gttctcgtcg acaatggcgg aactggcgac
      721 gtgactgtcg ccccaagcaa cttcgctaac ggggtcgctg aatggatcag ctctaactcg
      781 cgatcacagg cttacaaagt aacctgtagc gttcgtcaga gctctgcgca gaatcgcaaa
      841 tacaccatca aagtcgaggt gcctaaagtg gcaacccaga ctgttggtgg tgaagagctt
      901 cctgtagccg gatggagatc ttacttaaat atggaactaa ccattccaat tttcgccacg
      961 aattccgact gcgagcttat tgttaaggca atgcaaggtc tcctaaaaga tggaaacccg
     1021 attccctcgg ccatcgcggc caactccggc atctacggag gtggaggtgg atctggtgga
     1081 tctcctccac atatggctta cccatacgat gttccagatt acgctcctcc aaagcttgga
     1141 tccgaattcc cgggtcgact cgagatgagc ctgcagagcg cgcagtacct ccggcaggca
     1201 gaagtcctga aggctgacat gacagattct aagctgggtc cagctgaagt ctggacatcc
     1261 aggcaggctc tgcaggacct gtaccagaaa atgctagtta ccgatttgga atacgcttta
     1321 gacaagaaag tagaacagga tctctggaat cacgccttta agaatcagat cacaacacta
     1381 caaggccagg caaagaatcg agcaaatccg aatcggagtg aagttcaggc aaacctttct
     1441 ctgttcctag aggcagctag tggcttctat actcagttat tacaagaact gtgtacagta
     1501 tttaatgtag atttaccatg ccgtgtgaag tcttcccaat tgggaattat cagcaataaa
     1561 cagacgcata ccagcgccat agtgaagcca cagtctagct cctgttccta tatctgccag
     1621 cactgcctcg tccaccttgg agacattgct cgatacagaa accagaccag ccaggcagag
     1681 tcctactata ggcatgcagc tcagcttgtc ccctccaatg gtcagcctta taatcagttg
     1741 gctatcttag cttcttccaa aggagaccat ctgaccacaa ttttctacta ctgcagaagc
     1801 attgctgtga agttcccttt cccagctgcc tccactaatc tgcaaaaagc actttctaaa
     1861 gcactggaaa gccgagatga ggtgaaaacc aagtggggtg tttctgactt catcaaggcc
     1921 tttattaaat tccacggtca tgtgtacctg agtaagagct tggaaaagtt gagccctctt
     1981 cgagagaaat tggaagaaca gtttaagagg ctgctattcc aaaaagcttt caactctcag
     2041 cagttagttc atgtcactgt cattaacctg tttcaacttc atcaccttcg tgactttagc
     2101 aatgaaaccg agcagcacac ttatagccaa gatgagcagc tatgttggac acagttgctg
     2161 gccctcttta tgtcttttct tggcatcctg tgcaagtgtc ctctacagaa tgagtctcag
     2221 gaggagtcct acaatgccta tcctcttcca gcagtcaagg tctccatgga ctggctaaga
     2281 ctcagaccca gggtctttca ggaggcagtg gtggatgaaa gacagtacat ttggccctgg
     2341 ttgatttctc ttctgaatag tttccatccc catgaagagg acctctcaag tattagtgcg
     2401 acaccacttc cagaggagtt tgaattacaa ggatttttgg cattgagacc ttctttcagg
     2461 aacttggatt tttccaaagg tcaccagggt attacagggg acaaagaagg ccagcaacga
     2521 cgaatacgac agcaacgctt gatctctata ggcaaatgga ttgctgataa tcagccaagg
     2581 ctgattcagt gtgaaaatga ggtagggaaa ttgttgttta tcacagaaat cccagaatta
     2641 atactggaag accccagtga agccaaagag aacctcattc tgcaggaaac atctgtgata
     2701 gagtcgctgg ctgcagatgg gagcccaggg ctaaaatcag tgctatctac aagccgaaat
     2761 ttaagcaaca actgtgacac aggagagaag ccagtggtta ccttcaaaga aaacattaag
     2821 acacgagaag tgaacagaga ccaaggaaga agttttcctc ccaaagaggt aaaatcccag
     2881 acagaactaa gaaagactcc agtgtctgaa gccagaaaaa cacctgtaac tcaaacccca
     2941 actcaagcaa gtaactccca gttcatcccc attcatcacc ctggagcctt ccctcctctt
     3001 cccagcaggc cagggtttcc gcccccaaca tatgttatcc ccccgcctgt ggcattttct
     3061 atgggctcag gttacacctt cccagctggt gtttctgtcc caggaacctt tcttcagcct
     3121 acagctcact ctccagcagg aaaccaggtg caagctggga aacagtccca cattccttac
     3181 agccagcaac ggccctctgg accagggcca atgaaccagg gacctcaaca atcacagcca
     3241 ccttcccagc aaccccttac atctttacca gctcagccaa cagcacagtc tacaagccag
     3301 ctgcaggttc aagctctaac tcagcaacaa caatccccta caaaagctgt gccggctttg
     3361 gggaaaagcc cgcctcacca ctctggattc cagcagtatc aacaggcaga tgcctccaaa
     3421 cagctgtgga atccccctca ggttcaaggc ccattaggga aaattatgcc tgtgaaacag
     3481 ccctactacc ttcagaccca agaccccata aaactgtttg agccgtcatt gcaacctcct
     3541 gtaatgcagc agcagcctct agaaaaaaaa atgaagcctt ttcccatgga gccatataac
     3601 cataatccct cagaagtcaa ggtcccagaa ttctactggg attcttccta cagcatggct
     3661 gataacagat ctgtaatggc acagcaagca aacatagacc gcaggggcaa acggtcacca
     3721 ggaatcttcc gtccagagca ggatcctgta cccagaatgc cgtttgagaa atccttattg
     3781 gagaagccct cagagctcat gtcacattca tcctctttcc tgtccctcac cggattctct
     3841 ctcaatcagg aaagataccc aaataatagt atgttcaatg aggtatatgg gaaaaacctg
     3901 acatccagct ccaaagcaga actcagtccc tcaatggccc cccaggaaac atctctgtat
     3961 tccctttttg aagggactcc gtggtctcca tcacttcctg ccagttcaga tcattcaaca
     4021 ccagccagcc agtctcctca ttcctctaac ccaagcagcc tacccagctc tcctccaaca
     4081 cacaaccata attctgttcc attctccaat tttggaccca ttgggactcc agataacagg
     4141 gatagaagga ctgcagatcg gtggaaaact gataagccag ccatgggtgg gtttggcatt
     4201 gattatctct cagcaacgtc atcctctgag agcagttggc atcaggccag cactccgagt
     4261 ggcacctgga caggccatgg cccttccatg gaggattcct ctgctgtcct catggaaagc
     4321 ctaaagtcta tctggtccag ttccatgatg catcctggac cttctgctct ggagcagctg
     4381 ttaatgcagc agaagcagaa acagcaacgg ggacaaggca ccatgaaccc tccacactga
     4441 gcggccgcct agagggccct attctatagt gtcacctaaa tgctagagct cgctgatcag
     4501 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     4561 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     4621 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     4681 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     4741 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     4801 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4861 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4921 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4981 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     5041 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     5101 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     5161 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     5221 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5281 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     5341 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     5401 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     5461 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     5521 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     5581 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     5641 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     5701 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     5761 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     5821 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     5881 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     5941 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     6001 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     6061 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     6121 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     6181 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     6241 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     6301 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     6361 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     6421 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     6481 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     6541 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     6601 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     6661 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     6721 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     6781 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     6841 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6901 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     6961 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7021 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7081 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7141 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7201 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7261 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7321 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7381 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7441 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7501 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7561 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     7621 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7681 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7741 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7801 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7861 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7921 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7981 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8041 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8101 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8161 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8221 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8281 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8341 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8401 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8461 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8521 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8581 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8641 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8701 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8761 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8821 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     8881 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     8941 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     9001 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     9061 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     9121 atgtacgggc cagatatacg cgtt
//
