LOCUS       sequence-412554-        9977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTAB182.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9977)
  AUTHORS   Braun JE, Huntzinger E, Fauser M, Izaurralde E
  TITLE     GW182 proteins directly recruit cytoplasmic deadenylase complexes to
            miRNA targets.
  JOURNAL   Mol Cell. 2011 Oct 7;44(1):120-33.
  PUBMED    21981923
REFERENCE   2  (bases 1 to 9977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2011-10-7"; volume: "44(1)"; pages: "120-33"
COMMENT     Sequence Label: pT7-EGFP-C1-HsTAB182_K
FEATURES             Location/Qualifiers
     source          1..9977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     553..572
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        553..571
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      599..608
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(605..607,608..610,611..1321)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(605..607,608..610,611..1321)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(650..671)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(911..930)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1258..1279
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6705..6826
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6742..6761)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6796..6815
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6833..7288)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6970..6991)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7182..7201
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7315..7419
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7421..7778
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7421..7612
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7444..7464)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7629..7764
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7813..8607
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7813..8607
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7867..7886)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8477..8496
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8795..8814)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8839..8886
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9215..9803
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9704..9723
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      541 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      601 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      661 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      721 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      781 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      841 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      901 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
      961 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1021 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1081 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1141 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1201 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1261 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1321 gtccggactc agatctcgag ctcaagcttc gaattctgca gtcgacggta ccgcgggccc
     1381 atcaaaagtg tctactctca gggaaagctc agccatggct tccccactgc cccgggagat
     1441 ggaggaggag ctggtgccta ctggctctga gccaggtgac actcgggcca aaccccctgt
     1501 caagcccaaa ccccgggccc tgcctgccaa gccagccctg cctgccaaac ccagcctgct
     1561 ggtgcctgtt gggcctcggc ctccccgggg tcccctggct gagttgcctt ctgccaggaa
     1621 gatgaacatg ctggcaggac cccagcccta tggtggcagc aagcgccccc ttccctttgc
     1681 accaaggcct gcggttgagg cctccactgg aggagaagcc acccaagaga ctgggaaaga
     1741 ggaggctggg aaagaggagc cacccccttt gacaccccca gctcgatgtg cagccccagg
     1801 gggtgtacgg aaggcccctg cccctttccg cccagcctca gagcgcttcg cggccaccac
     1861 ggtggaagag atcctggcca agatggagca gcctcggaag gaggtccttg ccagccccga
     1921 ccgcctgtgg ggttcccgcc tcacctttaa ccacgatggc agctcgcgat atggccccag
     1981 gacctatggc acgaccactg ctcccaggga tgaggatggc agcaccctct tcaggggatg
     2041 gtcccaggag gggccagtaa agtctccagc agagtgccgg gaagagcaca gcaagacccc
     2101 tgaggagagg agccttcctt ccgacctggc cttcaacggg gacctggcta aggcagccag
     2161 ctcggagcta cctgctgata tttccaagcc ctggattccc tcaagtccag ccccctcctc
     2221 agagaatgga ggccctgcca gcccaggcct ccccgcagaa gcctcaggct caggccctgg
     2281 ctctccccat cttcacccgc ctgataagag ttctccctgc cactcacagc ttctggaagc
     2341 ccagactcct gaagcttccc aggcttctcc ctgccccgct gtgactccat cagctccaag
     2401 tgcagccctg cctgacgagg gctcccgcca cacccccagc ccggggctcc ctgccgaggg
     2461 ggctccagag gcccccagac ccagcagccc accccctgag gtcttggagc cccatagcct
     2521 ggatcagccc cctgccacct caccccggcc cctgatcgag gtgggtgagt tgctggatct
     2581 cactcggacg tttccatctg gcggggagga ggaggccaag ggtgacgcac acctccgccc
     2641 caccagcctg gttcagcgcc gattctctga aggtgtgctc cagtcaccca gtcaggacca
     2701 ggagaagctg gggggctcgc tggctgccct gccccaaggc caggggagcc agttggccct
     2761 ggatcgtccc tttggggcag agtccaactg gagcttatca cagtccttcg aatggacctt
     2821 ccccacgagg ccctcgggtc tgggcgtgtg gcggctggac tccccgcctc cctcccccat
     2881 cactgaagcc agtgaggccg ccgaggctgc tgaggctggc aacttggccg tttccagcag
     2941 ggaagaagga gtgtctcagc aggggcaagg ggctgggtca gctccaagtg ggtcaggaag
     3001 ttcctgggtg cagggggatg atccaagcat gtccctcacc cagaagggcg atggggagag
     3061 tcaacctcaa ttcccagctg ttccccttga gcccctgcct acaactgagg gcacacctgg
     3121 attacctttg cagcaggcag aggagagata cgagtcgcag gagcccttgg ctggacagga
     3181 gtcccctctc cccctggcta ccagggaggc agccttgccc atcctggagc cagtcctggg
     3241 gcaggagcag ccagcagccc ctgaccagcc ctgtgttctc tttgctgatg cccctgagcc
     3301 tggacaggca ctgcctgttg aggaggaggc cgtgacccta gcccgggctg agaccaccca
     3361 agccaggaca gaggctcaag acttgtgtag ggcatccccc gagcctccag gccctgaaag
     3421 cagctcccgc tggctggacg acctcctggc ttcaccacca cccagtggtg gcggtgcaag
     3481 gcggggagct ggagctgagc tgaaggacac acagtcccca agtacctgct ctgagggact
     3541 ccttggctgg tcccagaaag atctgcagag tgaatttggg atcacaggag acccacagcc
     3601 cagcagtttc agtccttcca gctggtgtca aggtgcttct caggactatg gccttggggg
     3661 tgcaagccct agaggagacc caggtctcgg agagagggac tggaccagca agtatgggca
     3721 aggagcaggg gaagggagca ccagggagtg ggccagcagg tgtggcatcg gccaggagga
     3781 gatggaggcc agcagcagcc aagaccagag taaagtgtct gccccagggg tgctcacagc
     3841 ccaggaccgg gtagttggaa agccagccca gcttggcact cagcggagcc aggaggcaga
     3901 tgttcaggac tgggagttca gaaagaggga ttcccagggc acttactcca gccgggatgc
     3961 agaactccag gaccaggaat tcggaaagag agattcactg ggtacctaca gtagtcgaga
     4021 tgtaagcctt ggggactggg aatttgggaa gagagattct ctgggtgctt atgccagcca
     4081 agatgccaac gagcagggcc aggatttggg gaagagggac caccatggta ggtacagcag
     4141 ccaggatgcc gatgagcagg actgggagtt tcagaagaga gatgtgtcac tcggcaccta
     4201 tggcagccgg gctgcggagc cacaggaaca ggagtttggg aagagcgctt ggataaggga
     4261 ctacagcagt ggtggcagct ccaggaccct tgacgcccag gacagaagct ttggaacgag
     4321 acccctgagc tctgggttca gccccgagga agcccagcaa caggatgagg aatttgagaa
     4381 gaagattcca agtgtggaag acagccttgg agagggcagc agggatgctg gccggccagg
     4441 agagagagga tccgggggct tgttcagtcc tagcactgcc cacgtgccgg atggggcact
     4501 cgggcagaga gaccagagca gctggcaaaa cagtgatgct agccaggagg tgggagggca
     4561 tcaggagaga cagcaggcag gggctcaggg ccctggcagt gctgacctgg aagatgggga
     4621 gatgggaaag cgaggctggg tcggtgagtt tagcctcagt gttggccccc agcgagaggc
     4681 agcatttagc ccagggcagc aggactggag ccgggacttc tgcatcgagg ccagtgagag
     4741 gagctatcag tttggcatca ttggcaacga cagagtgagt ggtgctggct ttagcccttc
     4801 tagcaagatg gaaggtggtc actttgtgcc tcctgggaag accacagctg gctcggtgga
     4861 ctggactgac cagctgggtc tcaggaactt ggaagtgtcc agctgtgtgg gttctggggg
     4921 ctctagcgag gccagggaga gtgccgtggg acagatgggc tggtcaggtg gcctgagctt
     4981 gagagacatg aacctgaccg gctgtttgga aagtggaggg tctgaagagc cggggggaat
     5041 cggagttggg gagaaggact ggacttctga tgttaatgtg aagagcaaag atttggctga
     5101 ggtcggggag ggaggaggcc acagccaggc cagagagagt ggcgtggggc agactgactg
     5161 gtcaggtgtg gaggccggag agttccttaa atcaagggag cgtggagttg gacaggcaga
     5221 ctggacacct gaccttgggc tgagaaacat ggccccaggg gcagtctgca gtcctggaga
     5281 gtccaaagag cttggggtgg gccagatgga ctggggtaac aatctgggcc tgagggattt
     5341 ggaggtgacc tgtgacccag actctggagg ttctcagggg ctacggggat gtggagtggg
     5401 gcagatggac tggacccagg acttggcgcc ccagaatgtg gagctctttg gggctccaag
     5461 tgaagccagg gagcatgggg tgggcggggt gagccagtgc ccagagcccg gcctgaggca
     5521 caatggcagc ttgtctcctg gcctggaggc cagagacccc ttggaggcca gggagctggg
     5581 ggttggtgag acaagtgggc cagagaccca gggtgaagat tactcctcgt cttccttgga
     5641 gccacaccct gcagaccctg gaatggagac aggagaagcc ctcagcttcg gagcaagccc
     5701 tggcaggtgc ccggcccgcc ccccaccctc cggctcccag ggcctgctgg aggagatgct
     5761 ggcagccagc agctccaagg cggtggctcg gagggagtca gcggcctcgg gccttggggg
     5821 cctgttggag gaggaaggag ccggggcagg tgctgcccaa gaggaggtgc tggagcctgg
     5881 cagggactct ccaccctcct ggaggccgca gcctgatggt gaggccagcc agacagaaga
     5941 cgtggatggc acctggggct cttcagcagc caggtggagc gatcaggggc cagcacagac
     6001 ttctcggcga ccctcccaag gccctcctgc cagatccccc agtcaggact tctccttcat
     6061 tgaggacacc gagatcctcg acagtgccat gtatcggagc cgtgccaact tggggcgcaa
     6121 gcgtgggcac cgggccccgg tcattcggcc tgggggtacc ttgggcctgt cggaggcagc
     6181 agactcggat gcacacctgt tccaggactc tacagagcca cgggcatctc gggtgccatc
     6241 ttcagatgaa gaggtagtgg aggaacctca gagccgccgg acacggatgt cgttgggcac
     6301 caaggggctg aaagtcaacc tctttcctgg cctgagcccc tcagccctga aggccaagct
     6361 gcgcccccgg aatcgctcag ctgaggaggg agagctggct gagagcaagt cgagccagaa
     6421 ggagtccgcg gtccagcgtt cgaaatcctg caaggtccca ggactgggaa agcccctcac
     6481 gttacctccc aagccagaga aatcctcagg gtcagaagga tcgtcgccca actggcttca
     6541 agccctgaaa ctgaagaaga agaaggtctg agatgggatc caccggatct agataactga
     6601 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     6661 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     6721 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     6781 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta
     6841 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     6901 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     6961 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     7021 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     7081 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     7141 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     7201 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     7261 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga
     7321 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     7381 ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc
     7441 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     7501 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     7561 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     7621 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7681 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     7741 agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga
     7801 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     7861 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     7921 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     7981 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     8041 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     8101 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     8161 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     8221 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     8281 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     8341 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     8401 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     8461 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     8521 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     8581 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     8641 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     8701 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     8761 tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg
     8821 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt
     8881 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
     8941 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
     9001 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt
     9061 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     9121 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     9181 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     9241 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     9301 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     9361 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     9421 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     9481 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     9541 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     9601 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     9661 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     9721 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     9781 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     9841 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     9901 gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc
     9961 ccatatatgg agttccg
//
