LOCUS       sequence-41257-e         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Garcia DM, Baek D, Shin C, Bell GW, Grimson A, Bartel DP
  TITLE     Weak seed-pairing stability and high target-site abundance decrease 
            the proficiency of lsy-6 and other microRNAs.
  JOURNAL   Nat Struct Mol Biol. 2011 Sep 11;18(10):1139-46. doi: 
            10.1038/nsmb.2115.
  PUBMED    21909094
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb"; 
            journalName: "Nat Struct Mol Biol"; date: "2011-09-11- 11"; volume: 
            "18"; issue: "10"; pages: "1139-46"
COMMENT     Sequence Label: pAG252
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    617..738
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(628..647)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     682..701
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctgaatttgt caagtaaaag gtcttcattt ttcgcaagaa gatgcacctg atgaaatggg
       61 aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat gaacaataat tctaggagct
      121 ctttgggatt gagcatcata ctggaaagca aacacctttc ctccagctcc agaattcctt
      181 gtctctgaat gactctgtct tgtgggtgtc tgacagtggc gacgatgaac atgccgttgg
      241 ttttattggc agtgggcaca aggaggtgag aagtggtggt aaaaggagcg gagtgctgaa
      301 gcagagagca gatttaatat agtaacatta acagtgtatt taattgacat ttcttttttg
      361 taatgtgacg atatgtggac aaagaagaag atgcaggttt aagaagttaa tatttataaa
      421 atgtgaaaga cacagttact aggataactt ttttgtgggt ggggcttggg agatggggtg
      481 gggtgggtta aggggtccca ttttgtttct ttggatttgg ggtgggggtc ctggccaaga
      541 actcagtcat ttttctgtgt accaggttgc ctaaatcaac tagttgttct agagcggccg
      601 cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
      661 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
      721 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
      781 gaggtgtggg aggtttttta aagcaagtaa aacctctac
//
