LOCUS       sequence-412591-       15822 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAVS1 targeting vector containing an all-in-one DOX-inducible system
            for CRISPRa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15822)
  AUTHORS   Petazzi P, Ventura T, Luongo FP, McClafferty H, May A, Taylor HA, 
            Shipston MJ, Romano N, Forrester LM, Menendez P, Fidanza A
  TITLE     A novel human pluripotent stem cell gene activation system 
            identifies IGFBP2 as a mediator in the production of haematopoietic 
            progenitors in vitro.
  JOURNAL   Elife. 2024 Dec 23;13:RP94884. doi: 10.7554/eLife.94884.
  PUBMED    39714446
REFERENCE   2  (bases 1 to 15822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2024-12-23"; volume: "13:RP94884. doi"; pages: " 
            10.7554/eLife.94884"
COMMENT     Sequence Label: AAVS1-iSAM
FEATURES             Location/Qualifiers
     source          1..15822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    2219..3055
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        3107..3456
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    3115..3133
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3151..3169
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3187..3205
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3223..3241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3259..3277
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3295..3313
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3331..3349
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             3514..3534
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3544..3567,3568..3570,3571..6057,6058..6060,
                     6061..7644)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             3544..7644
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7699..7719
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7744..7893
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             7912..7968
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7981..8367
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             8422..8442
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8458..9000
                     /codon_start=1
                     /product="C-terminal portion of the p65 subunit of mouse 
                     NF-kappa-B"
                     /label=p65
                     /translation="PSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTP
                     GPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSE
                     FQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDED
                     FSSIADMDFSALLSQISS"
     CDS             9025..9396
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             9412..9465
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             9466..10176
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(9611..9629)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    10193..10241
                     /note="synthetic polyadenylation signal"
     polyA_signal    10879..11013
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10916..10935)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10970..10989
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(11023..11769)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(11877..13059)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          11886..12828
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(11909..11929)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     polyA_signal    13321..13432
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     13421..13438
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(13454..14248)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13565..13584)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     14175..14194
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(14258..14311)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    14335..14360
                     /label=SA
                     /note="splice acceptor site"
     misc_feature    14361..15164
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(15178..15197)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(15179..15197)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(15207..15224)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(15207..15223)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15216..15238)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      15365..15820
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15452..15471)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15662..15683
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggag
     2221 agcagagcca ggaacccctg tagggaaggg gcaggagagc caggggcatg agatggtgga
     2281 cgaggaaggg ggacagggaa gcctgagcgc ctctcctggg cttgccaagg actcaaaccc
     2341 agaagcccag agcagggcct tagggaagcg ggaccctgct ctgggcggag gaatatgtcc
     2401 cagatagcac tggggactct ttaaggaaag aaggatggag aaagagaaag ggagtagagg
     2461 cggccacgac ctggtgaaca cctaggacgc accattctca caaagggagt tttccacacg
     2521 gacacccccc tcctcaccac agccctgcca ggacggggct ggctactggc cttatctcac
     2581 aggtaaaact gacgcacgga ggaacaatat aaattgggga ctagaaaggt gaagagccaa
     2641 agttagaact caggaccaac ttattctgat tttgtttttc caaactgctt ctcctcttgg
     2701 gaagtgtaag gaagctgcag caccaggatc agtgaaacgc accagacagc cgcgtcagag
     2761 cagctcaggt tctgggagag ggtagcgcag ggtggccact gagaaccggg caggtcacgc
     2821 atccccccct tccctcccac cccctgccaa gctctccctc ccaggatcct ctctggctcc
     2881 atcgtaagca aaccttagag gttctggcaa ggagagagat ggctccagga aatgggggtg
     2941 tgtcaccaga taaggaatct gcctaacagg aggtgggggt tagacccaat atcaggagac
     3001 taggaaggag gaggcctaag gatggggctt ttctgtcacc aatcctgtcc ctagtagcgg
     3061 ccggccagct ttccagttta tcgatgaggc cctttcgtct tcactcgagt ttactcccta
     3121 tcagtgatag agaacgtatg aagagtttac tccctatcag tgatagagaa cgtatgcaga
     3181 ctttactccc tatcagtgat agagaacgta taaggagttt actccctatc agtgatagag
     3241 aacgtatgac cagtttactc cctatcagtg atagagaacg tatctacagt ttactcccta
     3301 tcagtgatag agaacgtata tccagtttac tccctatcag tgatagagaa cgtatgtcga
     3361 ggtaggcgtg tacggtgggc gcctataaaa gcagagctcg tttagtgaac cgtcagatcg
     3421 cctggagcaa ttccacaaca cttttgtctt atacttggat cactagggcc gctagctcta
     3481 gagagcgtcg aattgtcgac ggccaccatg agccccaaga agaagagaaa ggtggaggcc
     3541 agcgacaaga agtacagcat cggcctggcc atcggcacca actctgtggg ctgggccgtg
     3601 atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg
     3661 cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag
     3721 gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc
     3781 tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga
     3841 ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc
     3901 aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag
     3961 aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac
     4021 atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac
     4081 gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc
     4141 atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga
     4201 cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggcaac
     4261 ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag
     4321 gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc
     4381 cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc
     4441 ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct
     4501 atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg
     4561 cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc
     4621 ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg
     4681 gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg
     4741 aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac
     4801 gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc
     4861 gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc
     4921 agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa
     4981 gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa cttcgataag
     5041 aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg
     5101 tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg
     5161 agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg gaaagtgacc
     5221 gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc
     5281 tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt
     5341 atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga agatatcgtg
     5401 ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa aacctatgcc
     5461 cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac cggctggggc
     5521 aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa gacaatcctg
     5581 gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat ccacgacgac
     5641 agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg cgatagcctg
     5701 cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat cctgcagaca
     5761 gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga gaacatcgtg
     5821 atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag ccgcgagaga
     5881 atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa agaacacccc
     5941 gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca gaatgggcgg
     6001 gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga tgtggacgct
     6061 atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct gaccagaagc
     6121 gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag
     6181 aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt cgacaatctg
     6241 accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat caagagacag
     6301 ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac
     6361 actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac cctgaagtcc
     6421 aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac
     6481 taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag
     6541 taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag
     6601 atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc
     6661 aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg
     6721 cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg ccgggatttt
     6781 gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg
     6841 cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga taagctgatc
     6901 gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc caccgtggcc
     6961 tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact gaagagtgtg
     7021 aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa tcccatcgac
     7081 tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag
     7141 tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc cggcgaactg
     7201 cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta cctggccagc
     7261 cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct gtttgtggaa
     7321 cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc caagagagtg
     7381 atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca ccgggataag
     7441 cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa tctgggagcc
     7501 cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac cagcaccaaa
     7561 gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga gacacggatc
     7621 gacctgtctc agctgggagg cgacagcgct ggaggaggtg gaagcggagg aggaggaagc
     7681 ggaggaggag gtagcggacc taagaaaaag aggaaggtgg cggccgctgg atccggacgg
     7741 gctgacgcat tggacgattt tgatctggat atgctgggaa gtgacgccct cgatgatttt
     7801 gaccttgaca tgcttggttc ggatgccctt gatgactttg acctcgacat gctcggcagt
     7861 gacgcccttg atgatttcga cctggacatg ctgattaacg gaggaagcgg agctactaac
     7921 ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc ctggacctgg aagcggaatg
     7981 gcttcaaact ttactcagtt cgtgctcgtg gacaatggtg ggacagggga tgtgacagtg
     8041 gctccttcta atttcgctaa tggggtggca gagtggatca gctccaactc acggagccag
     8101 gcctacaagg tgacatgcag cgtcaggcag tctagtgccc agaagagaaa gtataccatc
     8161 aaggtggagg tccccaaagt ggctacccag acagtgggcg gagtcgaact gcctgtcgcc
     8221 gcttggaggt cctacctgaa catggagctc actatcccaa ttttcgctac caattctgac
     8281 tgtgaactca tcgtgaaggc aatgcagggg ctcctcaaag acggtaatcc tatcccttcc
     8341 gccatcgccg ctaactcagg tatctacagc gctggaggag gtggaagcgg aggaggagga
     8401 agcggaggag gaggtagcgg acctaagaaa aagaggaagg tggcggccgc tggatcccct
     8461 tcagggcaga tcagcaacca ggccctggct ctggccccta gctccgctcc agtgctggcc
     8521 cagactatgg tgccctctag tgctatggtg cctctggccc agccacctgc tccagcccct
     8581 gtgctgaccc caggaccacc ccagtcactg agcgctccag tgcccaagtc tacacaggcc
     8641 ggcgagggga ctctgagtga agctctgctg cacctgcagt tcgacgctga tgaggacctg
     8701 ggagctctgc tggggaacag caccgatccc ggagtgttca cagatctggc ctccgtggac
     8761 aactctgagt ttcagcagct gctgaatcag ggcgtgtcca tgtctcatag tacagccgaa
     8821 ccaatgctga tggagtaccc cgaagccatt acccggctgg tgaccggcag ccagcggccc
     8881 cccgaccccg ctccaactcc cctgggaacc agcggcctgc ctaatgggct gtccggagat
     8941 gaggacttct caagcatcgc tgatatggac tttagtgccc tgctgtcaca gatttcctct
     9001 agtgggcagg gaggaggtgg aagcggcttc agcgtggaca ccagtgccct gctggacctg
     9061 ttcagcccct cggtgaccgt gcccgacatg agcctgcctg accttgacag cagcctggcc
     9121 agtatccaag agctcctgtc tccccaggag ccccccaggc ctcccgaggc agagaacagc
     9181 agcccggatt cagggaagca gctggtgcac tacacagcgc agccgctgtt cctgctggac
     9241 cccggctccg tggacaccgg gagcaacgac ctgccggtgc tgtttgagct gggagagggc
     9301 tcctacttct ccgaagggga cggcttcgcc gaggacccca ccatctccct gctgacaggc
     9361 tcggagcctc ccaaagccaa ggaccccact gtctccgcta gcggcagtgg agagggcaga
     9421 ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg gcccaatggt gagcaagggc
     9481 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     9541 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     9601 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     9661 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     9721 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     9781 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     9841 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaaaacc
     9901 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     9961 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
    10021 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
    10081 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
    10141 cactccaccg gcggcatgga cgagctgtac aagtagtgat aaaagcggcc gcaataaaat
    10201 atctttattt tcattacatc tgtgtgttgg ttttttgtgt gcccgagctt cctcgctcac
    10261 ttaattaagg cgcgcctgtc attctaaatc tctctttcag cctaaagctt tttccccgta
    10321 tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga aagcgatccc
    10381 gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg atgcgggggg
    10441 agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg ggggagggac
    10501 gtaattacat ccctgggggc tttgggaggg ggctgtcccc gtgagctccc aggcgcgcct
    10561 gtcattctaa atctctcttt cagcctaaag ctttttcccc gtatcccccc aggtgtctgc
    10621 aggctcaaag agcagcgaga agcgttcaga ggaaagcgat cccgtgccac cttccccgtg
    10681 cccgggctgt ccccgcacgc tgccggctcg gggatgcggg gggagcgccg gaccggagcg
    10741 gagccccggg cggctcgctg ctgcccccta gcgggggagg gacgtaatta catccctggg
    10801 ggctttgggg gggggctgtc cccgtgagct cccaggcgcg ccttaattaa tgacgtcgac
    10861 tcgctgcgct cggtatttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    10921 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    10981 aaactcatca atgtatctta tcatgtctgg atccttactt agttacccgg ggagcatgtc
    11041 aaggtcaaaa tcgtcaagag cgtcagcagg cagcatatca aggtcaaagt cgtcaagggc
    11101 atcggctggg agcatgtcta agtcaaaatc gtcaagggcg tcggtcggcc cgccgctttc
    11161 gcactttagc tgtttctcca ggccacatat gattagttcc aggccgaaaa ggaaggcagg
    11221 ttcggctccc tgccggtcga acagctcaat tgcttgtttc agaagtgggg gcatagaatc
    11281 ggtggtaggt gtctctcttt cctcttttgc tacttgatgc tcctgttcct ccaatacgca
    11341 gcccagtgta aagtggccca cggcggacag agcgtacagt gcgttctcca gggagaagcc
    11401 ttgctgacac aggaacgcga gctgattttc cagggtttcg tactgtttct ctgttgggcg
    11461 ggtgccgaga tgcactttag ccccgtcgcg atgtgagagg agagcacagc ggtatgactt
    11521 ggcgttgttc cgcagaaagt cttgccatga ctcgccttcc agggggcagg agtgggtatg
    11581 atgcctgtcc agcatctcga ttggcagggc atcgagcagg gcccgcttgt tcttcacgtg
    11641 ccagtacagg gtaggctgct caactcccag cttttgagcg agtttccttg tcgtcaggcc
    11701 ttcgataccg actccattga gtaattccag agcagagttt atgactttgc tcttgtccag
    11761 tctagacatg gtgaattcgg ggccgcggag gctggatcgg tcccggtgtc ttctatggag
    11821 gtcaaaacag cgtggatggc gtctccaggc gatctgacgg ttcactaaac gagctctcac
    11881 gacacctgaa atggaagaaa aaaactttga accactgtct gaggcttgag aatgaaccaa
    11941 gatccaaact caaaaagggc aaattccaag gagaattaca tcaagtgcca agctggccta
    12001 acttcagtct ccacccactc agtgtgggga aactccatcg cataaaaccc ctccccccaa
    12061 cctaaagacg acgtactcca aaagctcgag aactaatcga ggtgcctgga cggcgcccgg
    12121 tactccgtgg agtcacatga agcgacggct gaggacggaa aggccctttt cctttgtgtg
    12181 ggtgactcac ccgcccgctc tcccgagcgc cgcgtcctcc attttgagct ccctgcagca
    12241 gggccgggaa gcggccatct ttccgctcac gcaactggtg ccgaccgggc cagccttgcc
    12301 gcccagggcg gggcgataca cggcggcgcg aggccaggca ccagagcagg ccggccagct
    12361 tgagactacc cccgtccgat tctcggtggc cgcgctcgca ggccccgcct cgccgaacat
    12421 gtgcgctggg acgcacgggc cccgtcgccg cccgcggccc caaaaaccga aataccagtg
    12481 tgcagatctt ggcccgcatt tacaagacta tcttgccaga aaaaaagcgt cgcagcaggt
    12541 catcaaaaat tttaaatggc tagagactta tcgaaagcag cgagacaggc gcgaaggtgc
    12601 caccagattc gcacgcggcg gccccagcgc ccaagccagg cctcaactca agcacgaggc
    12661 gaaggggctc cttaagcgca aggcctcgaa ctctcccacc cacttccaac ccgaagctcg
    12721 ggatcaagaa tcacgtactg cagccagggg cgtggaagta attcaaggca cgcaagggcc
    12781 ataacccgta aagaggccag gcccgcggga accacacacg gcacttacct gtgttctggc
    12841 ggcaaacccg ttgcgaaaaa gaacgttcac ggcgactact gcacttatat acggttctcc
    12901 cccaccctcg ggaaaaaggc ggagccagta cacgacatca ctttcccagt ttaccccgcg
    12961 ccaccttctc taggcaccgg ttcaattgcc gacccctccc cccaacttct cggggactgt
    13021 gggcgatgtg cgctctgccc actgacgggc accggagcct cacgcatgct cttctccacc
    13081 tcagtgatga cgagagcggg cgggtgaggg ggcgggaacg cagcgatctc tgggttctac
    13141 gttagtggga gtttaacgac ggtccctggg attccccaag gcaggggcga gtccttttgt
    13201 atgaattact cgatatcatg agaattctag caccggttgc tattgtcttc ccaatcctcc
    13261 cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact cagacaatgc
    13321 gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc cagggtcaag
    13381 gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac agtcgaggct
    13441 gatcagcggg ttttcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat
    13501 cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt
    13561 cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc
    13621 cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat
    13681 cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc ctggcgaaca
    13741 gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg
    13801 cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg
    13861 tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg
    13921 caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt
    13981 cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca
    14041 gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac aggtcggtct
    14101 tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc
    14161 cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac
    14221 ctgcgtgcaa tccatcttgt tcagccattt cgagcctagg gccgggattc tcctccacgt
    14281 caccgcatgt tagaagactt cctctgccct ctccgctgcc agatctctcg aggccctgtg
    14341 ggaggaagag aagaggtcag gccccactgt ggggtggagg ggacagataa aagtacccag
    14401 aaccagagcc acattaaccg gccctgggaa tataaggtgg tcccagctcg gggacacagg
    14461 atccctggag gcagcaaaca tgctgtcctg aagtggacat aggggcccgg gttggaggaa
    14521 gaagactagc tgagctctcg gacccctgga agatgccatg acagggggct ggaagagcta
    14581 gcacagacta gagaggtaag gggggtaggg gagctgccca aatgaaagga gtgagaggtg
    14641 acccgaatcc acaggagaac ggggtgtcca ggcaaagaaa gcaagaggat ggagaggtgg
    14701 ctaaagccag ggagacgggg tactttgggg ttgtccagaa aaacggtgat gatgcaggcc
    14761 tacaagaagg ggaggcggga cgcaagggag acatccgtcg gagaaggcca tcctaagaaa
    14821 cgagagatgg cacaggcccc agaaggagaa ggaaaaggga acccagcgag tgaagacggc
    14881 atggggttgg gtgagggagg agagatgccc ggagaggacc cagacacggg gaggatccgc
    14941 tcagaggaca tcacgtggtg cagcgccgag aaggaagtgc tccggaaaga gcatccttgg
    15001 gcagcaacac agcagagagc aaggggaaga gggagtggag gaagacggaa cctgaaggag
    15061 gcggcaggga aggatctggg ccagccgtag aggtgaccca ggccacaagc tgcagacaga
    15121 aagcggcaca ggcccagggg agagaatgct ggtcagagaa agcaggtacc caattcgccc
    15181 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
    15241 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
    15301 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
    15361 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
    15421 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
    15481 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
    15541 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
    15601 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
    15661 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
    15721 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
    15781 tttaacgcga attttaacaa aatattaacg cttacaattt ag
//
