LOCUS       sequence-412613-        9608 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ATP8A1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9608)
  AUTHORS   Takatsu H, Baba K, Shima T, Umino H, Kato U, Umeda M, Nakayama K, 
            Shin HW
  TITLE     ATP9B, a P4-ATPase (a putative aminophospholipid translocase), 
            localizes to the trans-Golgi network in a CDC50 protein-independent 
            manner.
  JOURNAL   J Biol Chem. 2011 Nov 4;286(44):38159-38167. doi: 
            10.1074/jbc.M111.281006. Epub 2011 Sep 13.
  PUBMED    21914794
REFERENCE   2  (bases 1 to 9608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.281006"; journalName: "J Biol Chem"; date: 
            "2011-11-4- 4"; volume: "286"; issue: "44"; pages: "38159-38167"
COMMENT     Sequence Label: pCAG/ATP8A1-HA
FEATURES             Location/Qualifiers
     source          1..9608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             400..1203
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(463..482)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1073..1092
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1210..1258
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        1268..1647
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2867..2889
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2951..2970
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    3039..3063
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6562..6586)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6630..6656
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6744..6763)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6809..6864
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6863..6882)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7225..7241)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7225..7241)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7238..7260)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7249..7265
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7273..7279,7280..7297,7298..7303))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7318..7339
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7398..7593
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7444..7579
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7599..7733
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7636..7655)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7690..7709
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7801..7818)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7972..8560)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8052..8071)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8731..9522,9523..9591))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9354..9373
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9592..9608,1..88))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
       61 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
      121 gcagtgtggt tttcaagagg aagcaaaaag cctctccacc caggcctgga atgtttccac
      181 ccaatgtcga gcagtgtggt tttgcaagag gaagcaaaaa gcctctccac ccaggcctgg
      241 aatgtttcca cccaatgtcg agcaaacccc gcccagcgtc ttgtcattgg cgaattaatt
      301 cgaacacgca gatgcagtcg gggcggcgcg gtcccaggtc cacttcgcat attaaggtga
      361 cgcgtgtggc ctcgaacacc gagcgaccct gcagccaata tgggatcggc cattgaacaa
      421 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
      481 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
      541 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
      601 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
      661 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
      721 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
      781 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
      841 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
      901 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgatgatctc
      961 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     1021 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     1081 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     1141 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     1201 tgaggggatc ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcgg
     1261 atccgtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     1321 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1381 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     1441 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     1501 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     1561 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     1621 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
     1681 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
     1741 gcagcgatgg gggcgggggg gggggggggc gcgcgccggg gggggggggg gggggggggg
     1801 gggggggggg gggggggggg gggggggagg gggggggggg gggggggggg ggcgcccgga
     1861 tggttctctt tttgcgcggc gccgccgcgc cggggccccg ctgcaagagc cgatacgggg
     1921 gggggggggg ctgccggccg cgggcttcgc cccgtgcccc gctccgccgc cgcctcgcgc
     1981 cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct
     2041 tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg
     2101 cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt
     2161 gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt
     2221 gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg
     2281 gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg
     2341 ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc
     2401 ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg
     2461 gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc
     2521 ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg cccccggagc
     2581 gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag
     2641 agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc
     2701 cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc
     2761 ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc
     2821 tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg
     2881 cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta
     2941 cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaatttgct
     3001 agcgagctcg cggccgcaga tctctagtta agctatcaac aagtttgtac aaaaaagcag
     3061 gctctttaaa ggaaccaatt cagtcgactg gatccaccat gcccaccatg cggaggaccg
     3121 tgtcggagat ccgctcgcgc gccgaaggtt atgagaagac agatgatgtt tcagagaaga
     3181 cctcactggc tgaccaggag gaagtaagga ctattttcat caaccagccc cagctgacaa
     3241 aattctgcaa taaccatgtc agcactgcaa aatacaacat aatcacattc cttccaagat
     3301 ttctctactc tcagttcaga agagctgcta attcattttt tctctttatt gcactgctgc
     3361 agcaaatacc tgatgtgtca ccaacaggtc gttatacaac actggttcct ctcttattta
     3421 ttttagctgt ggcagctatc aaagagataa tagaagatat taaacgacat aaagctgata
     3481 atgcagtgaa caagaaacaa acgcaagttt tgagaaatgg tgcttgggaa attgtccact
     3541 gggaaaaggt aaatgttgga gatatagtta taataaaagg caaagagtat atacctgctg
     3601 acactgtact tctctcatca agtgagcccc aagccatgtg ctacattgaa acatccaact
     3661 tagatggtga aacaaacttg aaaattagac agggcttacc agcaacatca gatatcaaag
     3721 acgttgacag tttgatgagg atttctggca gaattgagtg tgaaagtcca aacagacatc
     3781 tctacgattt tgttggaaac ataaggcttg atggacatgg caccgttcca ctgggagcag
     3841 atcagattct tcttcgagga gctcagttga gaaatacaca gtgggttcat ggaatagttg
     3901 tctacactgg acatgacacc aagctgatgc agaattcaac aagtccacca cttaagctct
     3961 caaatgtgga acggattaca aatgtacaaa ttttgatttt attttgtatc ttaattgcca
     4021 tgtctcttgt ctgttctgtg ggctcagcca tttggaatcg aaggcattct ggaaaagact
     4081 ggtatctcaa tctaaactat ggtggcgcta gtaattttgg actgaatttc ttgaccttca
     4141 tcatcctttt caacaatctc attcctatca gcttattggt tacattagaa gttgtgaaat
     4201 ttacccaggc atacttcata aattgggatc ttgacatgca ctatgaaccc acagacactg
     4261 ctgctatggc tcgaacatct aatctgaatg aggaacttgg ccaggttaaa tacatatttt
     4321 ctgacaaaac tggtactctg acatgcaatg taatgcagtt taagaagtgc accatagcgg
     4381 gagttgctta tgggcagaac tcacagtttg gagatgaaaa aacatttagt gattcatcat
     4441 tgctggaaaa tctccaaaat aatcatccaa ctgcacctat aatatgtgaa tttcttacaa
     4501 tgatggcagt ctgtcacaca gcagtgccag agcgagaagg tgacaagatt atttatcaag
     4561 cagcatctcc agatgaggga gcattggtca gagcagccaa gcaattgaat tttgttttca
     4621 ctggaagaac acccgactcg gtgattatag attcactggg gcaggaagaa agatatgaat
     4681 tgctcaatgt cttggagttt accagtgcta ggaaaagaat gtcagtgatt gttcgcactc
     4741 catctggaaa gttacgactc tactgcaaag gagctgacac tgtaatttat gatcgactgg
     4801 cagagacgtc aaaatacaaa gaaattaccc taaaacattt agagcagttt gctacagaag
     4861 ggttaagaac tttatgtttt gctgtggctg agatttcaga gagcgacttt caggagtggc
     4921 gagcagtcta tcagcgagca tctacatctg tgcagaacag gctactcaaa ctcgaagaga
     4981 gttatgagtt gattgaaaag aatcttcagc tacttggagc aacagccatt gaggataaat
     5041 tacaagatca agtgcctgaa accatagaaa cgctaatgaa agcagacatc aaaatctgga
     5101 tccttacagg ggacaagcaa gaaactgcca ttaacatcgg acactcctgc aaactgttga
     5161 agaagaacat gggaatgatt gttataaatg aaggctctct tgatggaaca agggaaactc
     5221 tcagtcgtca ctgtactacc cttggtgatg ctctccggaa agagaatgat tttgctctta
     5281 taattgatgg gaaaaccctc aaatatgcct taacctttgg agtacgacag tatttcctgg
     5341 acttagcttt gtcatgcaaa gctgtcattt gctgtcgggt ttctcctctt caaaaatctg
     5401 aagttgttga gatggttaag aaacaagtca aagtcgtaac gcttgcaatc ggtgatggag
     5461 caaatgatgt cagcatgata cagacagcgc acgttggtgt tggtatcagt ggcaatgaag
     5521 gcctgcaggc agctaattcc tctgactact ccatagctca gttcaaatat ttgaagaatt
     5581 tactgatgat tcatggtgcc tggaactata acagagtctc caagtgcatc ttatactgct
     5641 tctacaagaa tatagtgctc tatattatcg agatctggtt tgcctttgtt aatggctttt
     5701 ctggacagat cctctttgaa agatggtgta taggtctcta taacgtgatg tttacagcaa
     5761 tgcctccttt aactcttgga atatttgaga gatcatgcag aaaagagaac atgttgaagt
     5821 accctgaatt atacaaaaca tctcagaatg ccctggactt caacaccaag gttttctggg
     5881 ttcattgttt aaatggcctc ttccactcag ttattctgtt ttggtttcca ctaaaagccc
     5941 ttcagtatgg tactgcattt ggaaatggga aaacctcgga ttatctgcta ctgggaaact
     6001 ttgtgtacac ttttgtggtg ataactgtgt gtttgaaagc tggattggag acatcatatt
     6061 ggacatggtt cagccacata gcgatatggg ggagcatcgc actctgggtg gtgttttttg
     6121 gaatctactc atctctgtgg cctgccattc cgatggcccc tgatatgtca ggagaggcag
     6181 ccatgttgtt cagttctgga gtcttttgga tgggcttgtt attcatccct gtggcatctc
     6241 tgctccttga tgtggtgtac aaggttatca agaggactgc ttttaaaaca ttggtcgatg
     6301 aagttcagga gctggaggca aaatctcaag acccaggagc agttgtactt ggaaaaagcc
     6361 tgaccgagag ggcgcaactg ctcaagaacg tctttaagaa gaaccacgtg aacttgtacc
     6421 gctctgaatc cttgcaacaa aatctgctcc atgggtatgc gttctctcaa gatgaaaatg
     6481 gaatcgtttc acagtctgaa gtgataagag catatgatac cacgaaacag aggcccgacg
     6541 aatgggcctc gagatatcta gacccagctt tcttgtacaa agtggttgat ctagagggcc
     6601 cgcggttcga actcgaggta ccgaattcgt acccatacga cgtcccagac tacgcttaat
     6661 tcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg
     6721 ctcacaaata ccactgagat cgatcttttt ccctctgcca aaaattatgg ggacatcatg
     6781 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg
     6841 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa
     6901 catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg
     6961 aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc
     7021 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta
     7081 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc
     7141 ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg
     7201 cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7261 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7321 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7381 gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     7441 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7501 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     7561 ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata
     7621 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7681 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca
     7741 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     7801 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7861 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7921 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7981 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     8041 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     8101 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     8161 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     8221 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     8281 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     8341 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     8401 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     8461 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     8521 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     8581 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8641 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8701 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8761 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8821 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8881 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8941 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     9001 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     9061 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     9121 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     9181 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     9241 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     9301 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     9361 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     9421 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     9481 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     9541 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9601 ttttcaat
//
