LOCUS       sequence-412624-        9719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ATP8B2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9719)
  AUTHORS   Takatsu H, Baba K, Shima T, Umino H, Kato U, Umeda M, Nakayama K, 
            Shin HW
  TITLE     ATP9B, a P4-ATPase (a putative aminophospholipid translocase), 
            localizes to the trans-Golgi network in a CDC50 protein-independent 
            manner.
  JOURNAL   J Biol Chem. 2011 Nov 4;286(44):38159-38167. doi: 
            10.1074/jbc.M111.281006. Epub 2011 Sep 13.
  PUBMED    21914794
REFERENCE   2  (bases 1 to 9719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.281006"; journalName: "J Biol Chem"; date: 
            "2011-11-4- 4"; volume: "286"; issue: "44"; pages: "38159-38167"
COMMENT     Sequence Label: pCAG/ATP8B2-HA
FEATURES             Location/Qualifiers
     source          1..9719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             400..1203
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(463..482)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1073..1092
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1210..1258
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        1268..1647
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2865..2887
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2949..2968
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    3037..3061
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6673..6697)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6741..6767
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6855..6874)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6920..6975
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6974..6993)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7336..7352)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7336..7352)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7349..7371)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7360..7376
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7384..7390,7391..7408,7409..7414))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7429..7450
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7509..7704
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7555..7690
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7710..7844
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7747..7766)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7801..7820
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7912..7929)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8083..8671)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8163..8182)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8842..9633,9634..9702))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9465..9484
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9703..9719,1..88))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
       61 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
      121 gcagtgtggt tttcaagagg aagcaaaaag cctctccacc caggcctgga atgtttccac
      181 ccaatgtcga gcagtgtggt tttgcaagag gaagcaaaaa gcctctccac ccaggcctgg
      241 aatgtttcca cccaatgtcg agcaaacccc gcccagcgtc ttgtcattgg cgaattaatt
      301 cgaacacgca gatgcagtcg gggcggcgcg gtcccaggtc cacttcgcat attaaggtga
      361 cgcgtgtggc ctcgaacacc gagcgaccct gcagccaata tgggatcggc cattgaacaa
      421 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
      481 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
      541 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
      601 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
      661 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
      721 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
      781 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
      841 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
      901 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgatgatctc
      961 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     1021 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     1081 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     1141 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     1201 tgaggggatc ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcgg
     1261 atccgtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     1321 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1381 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     1441 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     1501 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     1561 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     1621 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
     1681 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
     1741 gcagcgatgg gggcgggggg ggggggggcg ggcgccgggg gggggggggg gggggggggg
     1801 gggggggggg gggggggggg gggggggggg gggcgccaat ggacgcgggg gccacccggg
     1861 gattcctttt tttgccgtgg ggggggggcg gggggcccac aaaacagaga gcacgcggcg
     1921 cggggggggc gcgtgggcgg cccttcgccc cctgccccgc tccgccgccg cctcgcgccg
     1981 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
     2041 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
     2101 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
     2161 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
     2221 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     2281 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     2341 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     2401 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     2461 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     2521 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc
     2581 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     2641 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     2701 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     2761 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     2821 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     2881 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     2941 gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aatttgctag
     3001 cgagctcgcg gccgcagatc tctagttaag ctatcaacaa gtttgtacaa aaaagcaggc
     3061 tctttaaagg aaccaattca gtcgacacca tggcagtgtg tgcaaaaaag cgccccccag
     3121 aagaagaaag gagggcgcgg gctaatgacc gagaatacaa tgagaaattc cagtatgcga
     3181 gtaactgcat caagacctcc aagtacaata ttctcacctt cctgcctgtc aacctctttg
     3241 agcagttcca ggaagttgcc aacacttact tcctgttcct cctcattctg cagttgatcc
     3301 cccagatctc ttccctgtcc tggttcacca ccattgtgcc tttggttctt gtcctcacca
     3361 tcacagctgt taaagatgcc actgatgact atttccgcca caagagcgat aaccaggtga
     3421 ataaccgcca gtctcaggtg ctgatcaatg gaatcctcca gcaggagcag tggatgaatg
     3481 tctgtgttgg tgatattatc aagctagaaa ataaccagtt tgtggcggcg gatctcctcc
     3541 tcctttccag cagtgagccc catgggctgt gttacataga gacagcagaa cttgatggcg
     3601 agaccaacat gaaagtacgt caggcgattc cagtcacctc agaattggga gacatcagta
     3661 agcttgccaa gtttgacggt gaagtgatct gtgaacctcc caacaacaaa ctggacaaat
     3721 tcagcggaac cctctactgg aaggaaaata agttccctct gagcaaccag aacatgctgc
     3781 tgcggggctg tgtgctgcga aacaccgagt ggtgcttcgg gctggtcatc tttgcaggtc
     3841 ccgacactaa gctgatgcaa aacagcggca gaacaaagtt caaaagaacg agtatcgatc
     3901 gcctaatgaa taccctggtg ctctggattt ttggattcct ggtttgcatg ggggtgatcc
     3961 tggccattgg caatgccatc tgggagcacg aggtggggat gcgtttccag gtctacctgc
     4021 cgtgggatga ggcagtggac agtgccttct tctctggctt cctctccttc tggtcctaca
     4081 tcatcatcct caacaccgtt gtgcccattt cactctatgt cagtgtggag gtcatccgtc
     4141 tgggccacag ctacttcatc aactgggata agaagatgtt ctgcatgaag aagcggacgc
     4201 ctgcagaagc ccgcaccacc accctaaacg aggagctggg ccaggtggag tacatcttct
     4261 ccgacaagac gggcaccctc acccagaaca tcatggtttt caacaagtgc tccatcaatg
     4321 gccacagcta tggtgatgtg tttgacgtcc tgggacacaa agctgaattg ggagagaggc
     4381 ctgaacctgt tgacttctcc ttcaatcctc tggctgacaa gaagttctta ttttgggacc
     4441 ccagcctgct ggaggctgtc aagatcgggg acccccacac gcatgagttc ttccgcctcc
     4501 tttccctgtg tcatactgtc atgtcagaag aaaagaacga aggagagctg tactacaaag
     4561 ctcagtcccc agatgagggg gccctggtca ccgcagccag gaactttggt tttgttttcc
     4621 gctctcgcac ccccaaaaca atcaccgtcc atgagatggg cacagccatc acctaccagc
     4681 tgctggccat cctggacttc aacaacatcc gcaagcggat gtcggtcata gtgcggaatc
     4741 cagaggggaa gatccgactc tactgcaaag gggctgacac tatcctactg gacagactgc
     4801 accactccac tcaagagctg ctcaacacca ccatggacca ccttaatgag tacgcagggg
     4861 aagggctgag gaccctggtg ctggcctaca aggatctgga tgaagagtac tacgaggagt
     4921 gggctgagcg acgcctccag gccagcctgg cccaggacag ccgggaggac aggctggcta
     4981 gcatctatga ggaggttgag aacaacatga tgctgctggg tgcaacggcc attgaggaca
     5041 aacttcagca aggggttcca gagaccattg ccctcctgac actggccaac atcaagattt
     5101 gggtgctaac cggagacaag caagagacgg ctgtgaacat cggctattcc tgcaagatgc
     5161 tgacggatga catgactgag gttttcatag tcactggcca tactgtcctg gaggtgcggg
     5221 aggagctcag gaaagcccgg gagaagatga tggactcatc ccgctctgta ggcaacggct
     5281 tcacctatca ggacaagctt tcttcttcca agctaacttc tgtcctggag gccgttgctg
     5341 gggagtacgc cctggtcata aatggtcaca gcctggccca cgcactggag gcagacatgg
     5401 agctggagtt tctggagaca gcgtgtgcct gcaaagctgt catctgctgc cgggtgaccc
     5461 ccttgcagaa ggcacaggtg gtagaactgg tcaagaagta caagaaggct gtgacgcttg
     5521 ccattggaga cggagccaat gatgtcagca tgatcaaaac ggctcacatt ggtgtgggga
     5581 tcagtgggca ggaagggatc caggctgtct tggcctccga ttactccttc tcccagttca
     5641 agttcctgca gcgcctcctg ctggtgcatg ggcgctggtc ctacctgcga atgtgcaagt
     5701 ttctttgcta tttcttctac aaaaactttg ctttcaccat ggtccacttc tggtttggct
     5761 tcttctgtgg cttctcagcc cagaccgtct atgaccagta tttcatcacc ctgtataaca
     5821 tcgtgtacac ctccctgcca gtcctggcta tgggggtctt tgatcaggat gtccccgagc
     5881 agcggagcat ggagtaccct aagctgtatg agccgggcca gctgaacctt ctcttcaaca
     5941 agcgggagtt cttcatctgc atcgcccagg gcatctacac ctccgtgctc atgttcttca
     6001 ttccctatgg ggtgtttgct gatgccaccc gggatgatgg cactcagctg gctgactacc
     6061 agtcctttgc agtcactgtg gccacatcct tggtcattgt ggttagcgtg cagattgggc
     6121 tcgacacagg ctactggacg gccatcaacc acttcttcat ctggggaagc cttgctgttt
     6181 actttgccat cctctttgcc atgcacagca atgggctctt cgacatgttt cccaaccagt
     6241 tccggtttgt ggggaatgcc cagaacacct tggcccagcc cacggtgtgg ctgaccattg
     6301 tgctcaccac agtcgtctgc atcatgcccg tggttgcctt ccgattcctc aggctcaacc
     6361 tgaagccgga tctctccgac acggtccgct acacacagct cgtgaggaag aagcagaagg
     6421 cccagcaccg ctgcatgcgg cgggttggcc gcactggctc ccggcgctcc ggctatgcct
     6481 tctcccatca ggagggcttc ggggagctca tcatgtctgg caagaacatg cggctgagct
     6541 ctctcgcgct ctccagcttc accacccgct ccagctccag ctggattgag agcctgcgca
     6601 ggaagaagag tgacagtgcc agtagcccca gtggcggtgc cgacaagccc ctcaagggct
     6661 cgagatatct agacccagct ttcttgtaca aagtggttga tctagagggc ccgcggttcg
     6721 aactcgaggt accgaattcg tacccatacg acgtcccaga ctacgcttaa ttcactcctc
     6781 aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat
     6841 accactgaga tcgatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt
     6901 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat
     6961 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat
     7021 gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt
     7081 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat
     7141 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct
     7201 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga
     7261 ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag
     7321 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     7381 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7441 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7501 gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     7561 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7621 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     7681 tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat aatggttaca
     7741 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7801 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc attaatgaat
     7861 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7921 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     7981 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8041 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8101 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8161 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8221 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8281 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8341 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8401 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8461 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8521 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8581 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8641 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8701 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8761 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8821 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8881 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8941 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9001 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9061 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9121 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9181 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9241 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9301 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9361 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9421 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9481 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9541 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9601 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9661 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaat
//
