LOCUS       sequence-412634-        9955 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ATP8B1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Takatsu H, Baba K, Shima T, Umino H, Kato U, Umeda M, Nakayama K, 
            Shin HW
  TITLE     ATP9B, a P4-ATPase (a putative aminophospholipid translocase), 
            localizes to the trans-Golgi network in a CDC50 protein-independent 
            manner.
  JOURNAL   J Biol Chem. 2011 Nov 4;286(44):38159-38167. doi: 
            10.1074/jbc.M111.281006. Epub 2011 Sep 13.
  PUBMED    21914794
REFERENCE   2  (bases 1 to 9955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.281006"; journalName: "J Biol Chem"; date: 
            "2011-11-4- 4"; volume: "286"; issue: "44"; pages: "38159-38167"
COMMENT     Sequence Label: pCAG/ATP8B1-HA
FEATURES             Location/Qualifiers
     source          1..9955
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             400..1203
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(463..482)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1073..1092
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1210..1258
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        1268..1647
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1974..2990
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2914..2936
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2998..3017
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    3086..3110
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5244..5255
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             complement(5609..5620)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(6909..6933)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6977..7003
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(7091..7110)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7156..7211
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7210..7229)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7572..7588)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7572..7588)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7585..7607)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7596..7612
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7620..7626,7627..7644,7645..7650))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7665..7686
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7745..7940
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7791..7926
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7946..8080
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7983..8002)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8037..8056
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8148..8165)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8319..8907)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8399..8418)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9078..9869,9870..9938))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9701..9720
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9939..9955,1..88))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
       61 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
      121 gcagtgtggt tttcaagagg aagcaaaaag cctctccacc caggcctgga atgtttccac
      181 ccaatgtcga gcagtgtggt tttgcaagag gaagcaaaaa gcctctccac ccaggcctgg
      241 aatgtttcca cccaatgtcg agcaaacccc gcccagcgtc ttgtcattgg cgaattaatt
      301 cgaacacgca gatgcagtcg gggcggcgcg gtcccaggtc cacttcgcat attaaggtga
      361 cgcgtgtggc ctcgaacacc gagcgaccct gcagccaata tgggatcggc cattgaacaa
      421 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
      481 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
      541 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
      601 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
      661 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
      721 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
      781 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
      841 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
      901 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgatgatctc
      961 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     1021 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     1081 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     1141 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     1201 tgaggggatc ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcgg
     1261 atccgtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     1321 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1381 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     1441 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     1501 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     1561 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     1621 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
     1681 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
     1741 gcagcgatgg gggcgggggg gggggggtct cgcgcctgtt ggggcgtggc gggggggggg
     1801 ggtggggggg gggggggagg gagggggggg gggggggggg gggggggggg cggggggggg
     1861 cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
     1921 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
     1981 ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
     2041 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
     2101 gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg
     2161 aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg
     2221 tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc
     2281 gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg
     2341 ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
     2401 gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc
     2461 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg
     2521 ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc
     2581 ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc ggcggctgtc
     2641 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     2701 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
     2761 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     2821 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
     2881 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     2941 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     3001 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga atttgctagc gagctcgcgg
     3061 ccgcagatct ctagttaagc tatcaacaag tttgtacaaa aaagcaggct ctttaaagga
     3121 accaattcag tcgactggat ccatgagtac agaaagagac tcagaaacga cgtttgacga
     3181 ggattctcag cctaatgacg aagtggttcc ctacagtgat gatgaaacag aagatgaact
     3241 tgatgaccag gggtctgctg ttgaaccaga acaaaaccga gtcaacaggg aagcagagga
     3301 gaaccgggag ccattcagaa aagaatgtac atggcaagtc aaagcaaacg atcgcaagta
     3361 ccacgaacaa cctcacttta tgaacacaaa attcttgtgt attaaggaga gtaaatatgc
     3421 gaataatgca attaaaacat acaagtacaa cgcatttacc tttataccaa tgaatctgtt
     3481 tgagcagttt aagagagcag ccaatttata tttcctggct cttcttatct tacaggcagt
     3541 tcctcaaatc tctaccctgg cttggtacac cacactagtg cccctgcttg tggtgctggg
     3601 cgtcactgca atcaaagacc tggtggacga tgtggctcgc cataaaatgg ataaggaaat
     3661 caacaatagg acgtgtgaag tcattaagga tggcaggttc aaagttgcta agtggaaaga
     3721 aattcaagtt ggagacgtca ttcgtctgaa aaaaaatgac tttgttccag ctgacattct
     3781 cctgctgtct agctctgagc ctaacagcct ctgctatgtg gaaacagcag aactggacgg
     3841 agaaaccaat ttaaaattta agatgtcact tgaaatcaca gaccagtacc tccaaagaga
     3901 agatacattg gctacatttg atggttttat tgaatgtgaa gaacccaata accgactgga
     3961 taagtttaca ggaacactat tttggagaaa cacaagtttt cctttggatg ctgataaaat
     4021 tttgttacgt ggctgtgtaa ttaggaacac cgatttctgc cacggcttag tcatttttgc
     4081 aggtgctgac actaaaataa tgaagaatag tgggaaaacc agatttaaaa gaactaaaat
     4141 tgattacttg atgaactaca tggtttacac gatctttgtt gttcttattc tgctttctgc
     4201 tggtcttgcc atcggccatg cttattggga agcacaggtg ggcaattcct cttggtacct
     4261 ctatgatgga gaagacgata caccctccta ccgtggattc ctcattttct ggggctatat
     4321 cattgttctc aacaccatgg tacccatctc tctctatgtc agcgtggaag tgattcgtct
     4381 tggacagagt cacttcatca actgggacct gcaaatgtac tatgctgaga aggacacacc
     4441 cgcaaaagct agaaccacca cactcaatga acagctcggg cagatccatt atatcttctc
     4501 tgataagacg gggacactca cacaaaatat catgaccttt aaaaagtgct gtatcaacgg
     4561 gcagatatat ggggaccatc gggatgcctc tcaacacaac cacaacaaaa tagagcaagt
     4621 tgattttagc tggaatacat atgctgatgg gaagcttgca ttttatgacc actatcttat
     4681 tgagcaaatc cagtcaggga aagagccaga agtacgacag ttcttcttct tgctcgcagt
     4741 ttgccacaca gtcatggtgg ataggactga tggtcagctc aactaccagg cagcctctcc
     4801 cgatgaaggt gccctggtaa acgctgccag gaactttggc tttgccttcc tcgccaggac
     4861 ccagaacacc atcaccatca gtgaactggg cactgaaagg acttacaatg ttcttgccat
     4921 tttggacttc aacagtgacc ggaagcgaat gtctatcatt gtaagaaccc cagaaggcaa
     4981 tatcaagctt tactgtaaag gtgctgacac tgttatttat gaacggttac atcgaatgaa
     5041 tcctactaag caagaaacac aggatgccct ggatatcttt gcaaatgaaa ctcttagaac
     5101 cctatgcctt tgctacaagg aaattgaaga aaaagaattt acagaatgga ataaaaagtt
     5161 tatggctgcc agtgtggcct ccaccaaccg ggacgaagct ctggataaag tatatgagga
     5221 gattgaaaaa gacttaattc tcctgggagc tacagctatt gaagacaagc tacaggatgg
     5281 agttccagaa accatttcaa aacttgcaaa agctgacatt aagatctggg tgcttactgg
     5341 agacaaaaag gaaactgctg aaaatatagg atttgcttgt gaacttctga ctgaagacac
     5401 caccatctgc tatggggagg atattaattc tcttcttcat gcaaggatgg aaaaccagag
     5461 gaatagaggt ggcgtctacg caaagtttgc acctcctgtg caggaatctt tttttccacc
     5521 cggtggaaac cgtgccttaa tcatcactgg ttcttggttg aatgaaattc ttctcgagaa
     5581 aaagaccaag agaaataaga ttctgaagct gaagttccca agaacagaag aagaaagacg
     5641 gatgcggacc caaagtaaaa ggaggctaga agctaagaaa gagcagcggc agaaaaactt
     5701 tgtggacctg gcctgcgagt gcagcgcagt catctgctgc cgcgtcaccc ccaagcagaa
     5761 ggccatggtg gtggacctgg tgaagaggta caagaaagcc atcacgctgg ccatcggaga
     5821 tggggccaat gacgtgaaca tgatcaaaac tgcccacatt ggcgttggaa taagtggaca
     5881 agaaggaatg caagctgtca tgtcgagtga ctattccttt gctcagttcc gatatctgca
     5941 gaggctactg ctggtgcatg gccgatggtc ttacataagg atgtgcaagt tcctacgata
     6001 cttcttttac aaaaactttg cctttacttt ggttcatttc tggtactcct tcttcaatgg
     6061 ctactctgcg cagactgcat acgaggattg gttcatcacc ctctacaacg tgctgtacac
     6121 cagcctgccc gtgctcctca tggggctgct cgaccaggat gtgagtgaca aactgagcct
     6181 ccgattccct gggttataca tagtgggaca aagagactta ctattcaact ataagagatt
     6241 ctttgtaagc ttgttgcatg gggtcctaac atcgatgatc ctcttcttca tacctcttgg
     6301 agcttatctg caaaccgtag ggcaggatgg agaggcacct tccgactacc agtcttttgc
     6361 cgtcaccatt gcctctgctc ttgtaataac agtcaatttc cagattggct tggatacttc
     6421 ttattggact tttgtgaatg ctttttcaat ttttggaagc attgcacttt attttggcat
     6481 catgtttgac tttcatagtg ctggaataca tgttctcttt ccatctgcat ttcaatttac
     6541 aggcacagct tcaaacgctc tgagacagcc atacatttgg ttaactatca tcctgactgt
     6601 tgctgtgtgc ttactacccg tcgttgccat tcgattcctg tcaatgacca tctggccatc
     6661 agaaagtgat aagatccaga agcatcgcaa gcggttgaag gcggaggagc agtggcagcg
     6721 acggcagcag gtgttccgcc ggggcgtgtc aacgcggcgc tcggcctacg ccttctcgca
     6781 ccagcggggc tacgcggacc tcatctcctc cgggcgcagc atccgcaaga agcgctcgcc
     6841 gcttgatgcc atcgtggcgg atggcaccgc ggagtacagg cgcaccgggg actcgtcgag
     6901 atatctagac ccagctttct tgtacaaagt ggttgatcta gagggcccgc ggttcgaact
     6961 cgaggtaccg aattcgtacc catacgacgt cccagactac gcttaattca ctcctcaggt
     7021 gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca
     7081 ctgagatcga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
     7141 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     7201 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
     7261 atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc
     7321 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa
     7381 aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa
     7441 catccctaaa attttcctta catgttttac tagccagatt tttcctcctc tcctgactac
     7501 tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag cccaagcttg
     7561 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     7621 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     7681 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
     7741 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     7801 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     7861 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     7921 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata
     7981 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     8041 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc
     8101 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8161 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8221 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8281 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8341 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8401 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8461 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8521 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8581 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8641 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8701 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     8761 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8821 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8881 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8941 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9001 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9061 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9121 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9181 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9241 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9301 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9361 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9421 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9481 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9541 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9601 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9661 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9721 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9781 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9841 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9901 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaat
//
