LOCUS       sequence-412648-        9101 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cis-regulatory region of mouse Lepr gene driven a eGFP reporter with
            hGFP driven rtTA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9101)
  AUTHORS   Yuan S, Stewart KS, Yang Y, Abdusselamoglu MD, Parigi SM, Feinberg 
            TY, Tumaneng K, Yang H, Levorse JM, Polak L, Ng D, Fuchs E
  TITLE     Ras drives malignancy through stem cell crosstalk with the 
            microenvironment.
  JOURNAL   Nature. 2022 Nov 30. doi: 10.1038/s41586-022-05475-6.
  PUBMED    36450983
REFERENCE   2  (bases 1 to 9101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2022 Nov 30. doi: 10.1038/s41586-022-05475-6."
COMMENT     Sequence Label: Lepr peak reporter-eGFP
FEATURES             Location/Qualifiers
     source          1..9101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     426..444
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             529..1233
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     CDS             complement(join(1283..1996,1997..1999,2000..2002))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1286..1996,1997..1999,2000..2002))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1328..1349)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1677..1696
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1936..1957
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1999..2008
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2030..2225)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2044..2179
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     LTR             3274..3507
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3579..3700
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3616..3635)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3670..3689
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3740..3875
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3895..3914)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3896..3914)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3924..3941)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3924..3940)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3933..3955)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4082..4537
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4169..4188)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4379..4400
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4563..4667
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4668..4736,4737..5528)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4886..4905)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5699..6287
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6188..6207
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6441..6458
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6575..6596
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6611..6616,6617..6634,6635..6641)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6649..6665
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6654..6676
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6673..6689
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6673..6689
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6708..6728
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6710..6728
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6756..6982
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6983..7163
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7210..7335
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7828..8061
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8246..8290
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8588..8836
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     8588..8608
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8759..8778
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    8932..9049
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(9098..9101,1..507)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
ORIGIN
        1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
       61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
      121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
      181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
      241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
      301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
      361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
      421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
      481 gaccgaatca ccgacctctc tccccagggg gatccaccgg agcttaccat gtctaggctg
      541 gacaagagca aagtcataaa cggagctctg gaattactca atggtgtcgg tatcgaaggc
      601 ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc agcctaccct gtactggcac
      661 gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg agatgctgga caggcatcat
      721 acccacttct gccccctgga aggcgagtca tggcaagact ttctgcggaa caacgccaag
      781 tcataccgct gtgctctcct ctcacatcgc gacggggcta aagtgcatct cggcacccgc
      841 ccaacagaga aacagtacga aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc
      901 ttctccctgg agaacgcact gtacgctctg tccgccgtgg gccactttac actgggctgc
      961 gtattggagg aacaggagca tcaagtagca aaagaggaaa gagagacacc taccaccgat
     1021 tctatgcccc cacttctgag acaagcaatt gagctgttcg accggcaggg agccgaacct
     1081 gccttccttt tcggcctgga actaatcata tgtggcctgg agaaacagct aaagtgcgaa
     1141 agcggcgggc cgaccgacgc ccttgacgat tttgacttag acatgctccc agccgatgcc
     1201 cttgacgatt ttgaccttga catgctcccc ggggaaatgc atgtttaaac gactgctagc
     1261 gacttctaga gtcgcggccg ctttacttgt acagctcgtc catgccgaga gtgatcccgg
     1321 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
     1381 gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
     1441 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
     1501 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
     1561 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
     1621 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
     1681 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
     1741 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc
     1801 cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
     1861 tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga
     1921 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca
     1981 gctcctcgcc cttgctcacc atggtggcga ccggtggatc ccccagcttt ttgcaaaagc
     2041 ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct
     2101 cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg gcggaactgg
     2161 gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga
     2221 gatgcgaatc ttatagaaaa tgactgcctc aaggaaaagt gcagacctca aattctccat
     2281 ttctgttgga gtcccaagca ttgcttggag gctaaccaca gccatcaagc catcactttt
     2341 ggcaaagtgt accccaccta catatccccc acctagacac atttctgcac tatgacacag
     2401 catggagtgt ccacttcgcc ccaggctttg agtcagtcgc tccacatatc gtgtgcatgc
     2461 taattaaccg ggcacctttg aggctcaatc ctgctggcgt tttagctcag ctgtggtaaa
     2521 ggattacgaa ggagtaaatg gttaagaaga catcacaacc aatctgttaa gaactgtcac
     2581 cacactgaaa accatctcta taatgagaca gaatttacca gagcaaaaca gattaacacc
     2641 ctgactcact ccatattttt ggagggaaag gggggaaaaa tccctaaaag gaaatgtagt
     2701 gtaaaaaata aattaatgtg ttcacctatt ttctcttttg aaaaaaaatc aatagactgc
     2761 ccatttacac agatgcaagc cccactgagc acgatcaata acggctgagc ttaccatcac
     2821 atgaactggg tgaaatattt tatccaagta tttaggttgt cagattcaga acataagcat
     2881 ttcctgatcg ctacgttggt gatctggatg ctgcccttct tgaatgcctg ccagtgacct
     2941 ccatgattgc ctctctccag gatcgcctct ttcctcttca gcttttactc ttccactcct
     3001 atcgtgtaaa cactcaacta acagggatgt aacaaccaca ctgaatcaca ctgggtgact
     3061 tacgggcatc acagactcat ccaagaacca gccttgtggt tggtgaattg gactgtgacc
     3121 acattccagg aatgagttgc cggttgtgta tctacacaca cacacacaca cacacacaga
     3181 tacacacatg actggacttt gtggtacctt taagaccaat gacttacaag gcagctgtag
     3241 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     3301 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     3361 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     3421 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     3481 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     3541 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     3601 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     3661 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     3721 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     3781 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     3841 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     3901 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     3961 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     4021 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     4081 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     4141 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     4201 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     4261 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     4321 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     4381 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     4441 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     4501 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     4561 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4621 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4681 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4741 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     4801 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     4861 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     4921 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     4981 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5041 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5101 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5161 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5221 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5281 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5341 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5401 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5461 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5521 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     5581 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     5641 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5701 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5761 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5821 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     5881 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     5941 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6001 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6061 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6121 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6181 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6241 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6301 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6361 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     6421 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     6481 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     6541 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     6601 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     6661 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     6721 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     6781 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     6841 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     6901 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     6961 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     7021 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7081 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7141 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     7201 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     7261 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     7321 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     7381 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     7441 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     7501 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     7561 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     7621 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     7681 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     7741 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     7801 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     7861 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     7921 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     7981 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     8041 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     8101 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     8161 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     8221 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     8281 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     8341 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     8401 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     8461 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     8521 tcgattagtg aacggatctc gacggtatcg atcacgagac tagcctcgag cggccgcccc
     8581 cttcaccgag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt
     8641 tagagagata attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
     8701 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
     8761 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
     8821 gaaaggacga aacaccggca acaagatgaa gagcaccaac tcgagttggt gctcttcatc
     8881 ttgttgtttt taattctcga cctcgagaca aatggcagta ttcatccaca attttaaaag
     8941 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     9001 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     9061 cagggacagc agagatccac tttggccgcg gctcgagggg g
//
