LOCUS       sequence-412649-        9733 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ATP8B4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9733)
  AUTHORS   Takatsu H, Baba K, Shima T, Umino H, Kato U, Umeda M, Nakayama K, 
            Shin HW
  TITLE     ATP9B, a P4-ATPase (a putative aminophospholipid translocase), 
            localizes to the trans-Golgi network in a CDC50 protein-independent 
            manner.
  JOURNAL   J Biol Chem. 2011 Nov 4;286(44):38159-38167. doi: 
            10.1074/jbc.M111.281006. Epub 2011 Sep 13.
  PUBMED    21914794
REFERENCE   2  (bases 1 to 9733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.281006"; journalName: "J Biol Chem"; date: 
            "2011-11-4- 4"; volume: "286"; issue: "44"; pages: "38159-38167"
COMMENT     Sequence Label: pCAG/ATP8B4-HA
FEATURES             Location/Qualifiers
     source          1..9733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             400..1203
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(463..482)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1073..1092
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1210..1258
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        1268..1647
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1649..1925
                     /label=chicken beta-actin promoter
     intron          1926..2942
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2866..2888
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2950..2969
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    3038..3062
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6687..6711)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6755..6781
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6869..6888)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6934..6989
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6988..7007)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7350..7366)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7350..7366)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7363..7385)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7374..7390
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7398..7404,7405..7422,7423..7428))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7443..7464
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7523..7718
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7569..7704
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7724..7858
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7761..7780)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7815..7834
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7926..7943)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8097..8685)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8177..8196)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8856..9647,9648..9716))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9479..9498
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9717..9733,1..88))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
       61 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
      121 gcagtgtggt tttcaagagg aagcaaaaag cctctccacc caggcctgga atgtttccac
      181 ccaatgtcga gcagtgtggt tttgcaagag gaagcaaaaa gcctctccac ccaggcctgg
      241 aatgtttcca cccaatgtcg agcaaacccc gcccagcgtc ttgtcattgg cgaattaatt
      301 cgaacacgca gatgcagtcg gggcggcgcg gtcccaggtc cacttcgcat attaaggtga
      361 cgcgtgtggc ctcgaacacc gagcgaccct gcagccaata tgggatcggc cattgaacaa
      421 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
      481 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
      541 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
      601 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
      661 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
      721 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
      781 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
      841 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
      901 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgatgatctc
      961 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     1021 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     1081 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     1141 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     1201 tgaggggatc ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcgg
     1261 atccgtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     1321 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1381 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     1441 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     1501 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     1561 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     1621 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc
     1681 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt
     1741 gcagcgatgg gggcgggggg gggggggggg gcgcgccggg gggggggggg gggggggggg
     1801 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
     1861 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
     1921 gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc
     1981 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt
     2041 ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc
     2101 gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg
     2161 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg
     2221 agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg
     2281 ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg
     2341 gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc
     2401 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
     2461 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
     2521 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
     2581 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
     2641 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
     2701 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     2761 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
     2821 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     2881 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     2941 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaatttgcta
     3001 gcgagctcgc ggccgcagat ctctagttaa gctatcaaca agtttgtaca aaaaagcagg
     3061 ctctttaaag gaaccaattc agtcgactgg atccaccatg ttctgcagtg aaaagaaatt
     3121 gcgtgaagtg gaacggatag tgaaagccaa tgaccgtgaa tataatgaaa agttccagta
     3181 tgcggataat cgtatccaca catcgaaata taatattctc accttcttgc caattaattt
     3241 atttgaacag ttccaaagag tggcaaatgc ctattttctt tgccttctga ttttacagct
     3301 aattccagaa atttcctcct tgacctggtt taccaccatt gtgcctttgg tcctggtgat
     3361 aactatgaca gctgtcaaag atgccacaga tgactatttt cgccacaaga gtgataatca
     3421 agtgaataat cggcagtctg aagtgctcat caacagcaaa ctgcagaatg aaaaatggat
     3481 gaatgtcaaa gtgggagaca tcattaaatt agaaaataac caatttgttg ctgctgattt
     3541 acttctccta tcaagtagtg agccacatgg tctctgttat gttgaaactg ctgagcttga
     3601 tggggaaacg aacctaaaag tccgccatgc actatcagtt acttcagaac ttggagcaga
     3661 tatcagcaga cttgcagggt ttgatgggat tgttgtctgt gaggtgccta acaacaagtt
     3721 agataaattc atgggaatcc tttcttggaa agacagcaag cattccctca acaatgagaa
     3781 gataatcctg agaggctgca tcctgagaaa taccagctgg tgttttggaa tggttatttt
     3841 tgcaggtcct gacactaaac taatgcagaa tagtggtaag acaaagttta aaaggacaag
     3901 cattgataga ttgatgaata ctctagtact atggattttt gggtttctga tatgcttggg
     3961 aattattctt gcaataggaa attcaatctg ggagagtcaa actggggacc aattcagaac
     4021 tttcctcttt tggaatgaag gagagaagag ctctgtgttc tccggattct taacattctg
     4081 gtcatatatt attattctca atacagttgt acccatttcc ttatatgtga gtgtggaagt
     4141 aattcgtcta ggacacagtt attttataaa ctgggaccgg aagatgtatt attctcgaaa
     4201 agcaatacct gcagtggctc gaacgaccac gctcaatgag gaactggggc agattgagta
     4261 cattttctcc gacaaaacgg gtaccctcac tcaaaacatc atgaccttta aaagatgttc
     4321 cattaatggg agaatctatg gtgaagtaca tgatgacctg gatcagaaga cagaaataac
     4381 tcaggaaaaa gagcctgtgg atttctcagt caaatctcaa gcggatagag aatttcagtt
     4441 ctttgaccac catctgatgg aatccattaa aatgggtgat cccaaagttc atgaattcct
     4501 taggttactt gctctctgcc acactgtaat gtcagaagag aatagcgcag gagagctgat
     4561 ttaccaagtt cagtcacctg atgaaggggc tctagtgact gccgctagaa attttgggtt
     4621 catttttaaa tcccggaccc cagagaccat aacaatagaa gaattgggaa cactagttac
     4681 ttatcaatta cttgcctttt tggatttcaa caacaccaga aaaaggatgt ctgtcatagt
     4741 tcgaaaccca gaaggacaga taaagcttta ttccaaagga gcagatacta ttctgtttga
     4801 aaaacttcat ccttccaatg aagtcctttt gtctttgacg tcagaccacc tcagtgaatt
     4861 tgcaggggaa ggccttcgga ccttggccat cgcatacaga gacctggatg acaagtactt
     4921 taaagagtgg cataagatgc ttgaagatgc gaatgctgcc acagaagaga gggatgaacg
     4981 aatagctgag ctatatgaag aaattgaaag agatttgatg ctactaggtg ccactgctgt
     5041 agaagataag ttacaggagg gtgttattga aacagttaca agtttatcac tagccaatat
     5101 taagatctgg gtcctaacag gagacaaaca agaaactgcc atcaacatcg gttatgcctg
     5161 caacatgctg actgacgaca tgaatgatgt gtttgtgata gcagggaata atgctgtgga
     5221 agtgagagaa gaactcagga aagcaaaaca aaatttgttt ggacaaaaca gaaatttttc
     5281 caatggccat gtagtttgtg aaaaaaagca gcagctggag ttggattcta ttgtagaaga
     5341 aaccataaca ggagattatg ccttaatcat aaatggccac agtttggctc atgccctaga
     5401 aagtgatgtc aagaatgatc tcctagaact tgcttgcatg tgtaagactg taatttgctg
     5461 cagggtcact ccactccaga aagcccaagt ggtagagctg gtgaagaagt acagaaatgc
     5521 tgttactttg gccattggtg atggagccaa tgatgtcagc atgattaaaa gtgctcacat
     5581 tggtgttggc atcagcggcc aggaaggatt gcaagcagtc ttagccagcg actattcatt
     5641 tgcacagttt agatatctcc aaaggcttct ccttgttcat ggaaggtggt cttatttccg
     5701 aatgtgcaaa ttcttatgct atttcttcta taagaatttt gcatttacac ttgtgcattt
     5761 ctggtttggt ttcttctgtg gtttctcagc ccagactgtt tatgaccagt ggttcatcac
     5821 cctttttaac attgtttaca catcactgcc tgttttagcc atggggattt ttgaccagga
     5881 tgtgagtgac cagaacagcg tggactgtcc ccagctctac aaaccaggac agctgaatct
     5941 gctttttaac aagcgtaaat ttttcatttg cgtgttgcat ggaatctaca cctcattagt
     6001 ccttttcttc atcccctatg gggcctttta caacgtggct ggagaagatg ggcaacatat
     6061 tgctgactac cagtcctttg cagttaccat ggccacatct ttggtcattg tggtcagtgt
     6121 gcagatagcc ttggatacca gttactggac tttcattaat cacgtcttca tctgggggag
     6181 cattgccatt tatttctcca ttttatttac aatgcacagt aatggcatct ttggcatctt
     6241 cccaaaccag tttccatttg ttggtaatgc acgacattcc ctgacccaga agtgcatctg
     6301 gcttgtaatt ctcttaacaa cagtggcttc agttatgcca gtggtggcat tcagattttt
     6361 gaaggtggat ttatacccaa ccctgagtga tcagatccgc cggtggcaga aggctcaaaa
     6421 gaaggcaagg cctccaagta gccgaaggcc tcggacccgc aggtcaagct caagaaggtc
     6481 tggatatgct tttgctcacc aagaaggcta tggagagctt atcacatctg gaaaaaatat
     6541 gcgagctaaa aatccacccc caacatcagg gctggaaaag acacattata atagcactag
     6601 ctggattgaa aatttatgta agaaaaccac agacaccgtg agcagcttta gccaggataa
     6661 aacagtgaaa ctctcgagat atctagaccc agctttcttg tacaaagtgg ttgatctaga
     6721 gggcccgcgg ttcgaactcg aggtaccgaa ttcgtaccca tacgacgtcc cagactacgc
     6781 ttaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
     6841 cctggctcac aaataccact gagatcgatc tttttccctc tgccaaaaat tatggggaca
     6901 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa
     6961 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt
     7021 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg
     7081 ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc
     7141 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg
     7201 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt
     7261 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg
     7321 acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7381 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7441 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7501 aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa
     7561 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7621 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     7681 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc
     7741 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7801 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg
     7861 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7921 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7981 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8041 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8101 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8161 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8221 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8281 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8341 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8401 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8461 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8521 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8581 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8641 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8701 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8761 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8821 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8881 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8941 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9001 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9061 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9121 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9181 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9241 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9301 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9361 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9421 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9481 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9541 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9601 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9661 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9721 ttcctttttc aat
//
