LOCUS       sequence-41265-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Garcia DM, Baek D, Shin C, Bell GW, Grimson A, Bartel DP
  TITLE     Weak seed-pairing stability and high target-site abundance decrease 
            the proficiency of lsy-6 and other microRNAs.
  JOURNAL   Nat Struct Mol Biol. 2011 Sep 11;18(10):1139-46. doi: 
            10.1038/nsmb.2115.
  PUBMED    21909094
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb"; 
            journalName: "Nat Struct Mol Biol"; date: "2011-09-11- 11"; volume: 
            "18"; issue: "10"; pages: "1139-46"
COMMENT     Sequence Label: pAG253
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    577..698
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(588..607)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     642..661
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctgaatttgt caaagtaaaa ggtcttcatt tttcgcaaga agatgcacct gatgaaatgg
       61 gaaaatatat caaatcgttc gttgagcgag ttctcaaaaa tgaacaataa ttctaggagc
      121 tcgccagatt acaggaatga agaactgttg cctaattgga aagtgccagc attccagact
      181 tcaaaaataa aaaatctgaa gaggcttctc ctgttttata tattatgtga agaatttaga
      241 tcttatattg gtttgcacaa gttccctgga gaaaaaaatt gctctgtgta tatctcttgg
      301 aaaataagac aatagtattt ctcctttgca atagcagtta taacagatgt gaaaatatac
      361 ttgactctaa tatgattata caaaagagca tggatgcatt tcaaatgtta gatattgcta
      421 ctataatcaa atgatttcat attgatcttt ttatcatgat cctccctatc aagcactaaa
      481 aagttgaacc attatacttt atatctgtaa tgatactgat tatgaaatgt cccctgaaac
      541 tagttgttct agagcggccg cttcgagcag acatgataag atacattgat gagtttggac
      601 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
      661 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
      721 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
      781 aatgtggtaa aatcgataag gatccaggtg gcacttttcg gggaaatgtg cgcggaaccc
      841 ctatttgttt atttttctaa atacattc
//
