LOCUS       sequence-41266-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Garcia DM, Baek D, Shin C, Bell GW, Grimson A, Bartel DP
  TITLE     Weak seed-pairing stability and high target-site abundance decrease 
            the proficiency of lsy-6 and other microRNAs.
  JOURNAL   Nat Struct Mol Biol. 2011 Sep 11;18(10):1139-46. doi: 
            10.1038/nsmb.2115.
  PUBMED    21909094
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb"; 
            journalName: "Nat Struct Mol Biol"; date: "2011-09-11- 11"; volume: 
            "18"; issue: "10"; pages: "1139-46"
COMMENT     Sequence Label: pCS253
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    576..697
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(587..606)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     641..660
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctgaatttgt caagtaaaag gtcttcattt ttcgcaagaa gatgcacctg atgaaatggg
       61 aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat gaacaataat tctaggagct
      121 cgccagatta caggaatgaa gaactgttgc ctaattggaa agtgccagca ttccagactt
      181 caaaaataaa aaatctgaag aggcttctcc tgttttatat attacgcgaa gaatttagat
      241 cttatattgg tttgcacaag ttccctggag aaaaaaattg ctctgtgtat atctcttgga
      301 aaataagaca atagtatttc tcctttgcaa tagcagttat aacagacgcg aaaatatact
      361 tgactctaat atgattatac aaaagagcat ggatgcattt caaatgttag atattgctac
      421 tataatcaaa tgatttcata ttgatctttt tatcatgatc ctccctatca agcactaaaa
      481 agttgaacca ttatacttta tatctgtaat gatactgatt atgaaatgtc ccctgaaact
      541 agttgttcta gagcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca
      601 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
      661 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
      721 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
      781 atgtggtaaa atcgataagg atccaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      841 tatttg
//
