LOCUS       sequence-412662-       10552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ATP10B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10552)
  AUTHORS   Takatsu H, Baba K, Shima T, Umino H, Kato U, Umeda M, Nakayama K, 
            Shin HW
  TITLE     ATP9B, a P4-ATPase (a putative aminophospholipid translocase), 
            localizes to the trans-Golgi network in a CDC50 protein-independent 
            manner.
  JOURNAL   J Biol Chem. 2011 Nov 4;286(44):38159-38167. doi: 
            10.1074/jbc.M111.281006. Epub 2011 Sep 13.
  PUBMED    21914794
REFERENCE   2  (bases 1 to 10552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.281006"; journalName: "J Biol Chem"; date: 
            "2011-11-4- 4"; volume: "286"; issue: "44"; pages: "38159-38167"
COMMENT     Sequence Label: pCAG/ATP10B-HA
FEATURES             Location/Qualifiers
     source          1..10552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             400..1203
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(463..482)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1073..1092
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1210..1258
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     intron          1926..2942
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2866..2888
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2950..2969
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    3038..3062
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(4145..4156)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7506..7530)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7574..7600
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(7688..7707)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7753..7808
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7807..7826)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8169..8185)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8169..8185)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8182..8204)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8193..8209
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8217..8223,8224..8241,8242..8247))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8262..8283
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8342..8537
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8388..8523
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8543..8677
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8580..8599)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8634..8653
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8745..8762)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8916..9504)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8996..9015)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9675..10466,10467..10535))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10298..10317
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10536..10552,1..88))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
       61 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
      121 gcagtgtggt tttcaagagg aagcaaaaag cctctccacc caggcctgga atgtttccac
      181 ccaatgtcga gcagtgtggt tttgcaagag gaagcaaaaa gcctctccac ccaggcctgg
      241 aatgtttcca cccaatgtcg agcaaacccc gcccagcgtc ttgtcattgg cgaattaatt
      301 cgaacacgca gatgcagtcg gggcggcgcg gtcccaggtc cacttcgcat attaaggtga
      361 cgcgtgtggc ctcgaacacc gagcgaccct gcagccaata tgggatcggc cattgaacaa
      421 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
      481 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
      541 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
      601 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
      661 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
      721 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
      781 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
      841 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
      901 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgatgatctc
      961 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     1021 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     1081 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     1141 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     1201 tgaggggatc ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcgg
     1261 atccgtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     1321 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1381 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     1441 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     1501 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     1561 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     1621 tacgtgtcgg tcacacgtat ggccaggccc cggcaacccg ccacgttccc tctcccagcc
     1681 ccctcacccc ccccgccccc ccccccatta ttgtatatgt ttttttttgg atttttgggg
     1741 ggggcggggg gggcgggggg gggggggggc gggcgcgggg gggggggggg cggggcgagg
     1801 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
     1861 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
     1921 gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc
     1981 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt
     2041 ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc
     2101 gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg
     2161 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg
     2221 agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg
     2281 ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg
     2341 gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc
     2401 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
     2461 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
     2521 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
     2581 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
     2641 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
     2701 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     2761 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
     2821 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     2881 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     2941 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaatttgcta
     3001 gcgagctcgc ggccgcagat ctctagttaa gctatcaaca agtttgtaca aaaaagcagg
     3061 ctctttaaag gaaccaattc agtcgactgg atccggtacc gaattcacca tggccctctc
     3121 agtggactca tcgtggcatc ggtggcagtg gagagtcaga gatggcttcc cccattgtcc
     3181 atcggaaacc acaccgctgc tctctccaga gaaagggaga cagagctaca acttgacaca
     3241 gcagcgggtc gtgttcccca acaacagcat attccatcaa gattgggaag aggtctccag
     3301 gagataccct ggcaacagaa cctgcacaac caaatacacc ctcttcacct tcctgccccg
     3361 gaatctcttt gagcaatttc atagatgggc taacctctat ttcctgttcc tggtgatttt
     3421 gaactggatg ccctccatgg aagtcttcca cagagaaatc accatgttac cattggccat
     3481 tgtcctgttc gtcatcatga tcaaggatgg catggaggac ttcaagagac accgctttga
     3541 taaagcaata aactgctcca acattcgaat ttatgaaaga aaagagcaga cctatgtgca
     3601 gaagtgctgg aaggatgtgc gtgtgggaga cttcatccaa atgaaatgca atgagattgt
     3661 cccagcagac atactcctcc ttttttcctc tgaccccaat gggatatgcc atctggaaac
     3721 tgccagcttg gatggagaga caaacctcaa gcaaagacgt gtcgtgaagg gcttctcaca
     3781 gcaggaggta cagttcgaac cagagctttt ccacaatacc atcgtgtgtg agaaacccaa
     3841 caaccacctc aacaaattta agggttatat ggagcatcct gaccagacca ggactggctt
     3901 tggctgtgag agtcttctgc ttcgaggctg caccatcaga aacaccgaga tggctgttgg
     3961 cattgtcatc tatgcaggcc atgagacgaa agccatgctg aacaacagtg gcccccggta
     4021 caaacgcagc aagattgagc ggcgcatgaa tatagacatc ttcttctgca ttgggatcct
     4081 catcctcatg tgccttattg gagctgtagg tcacagcatc tggaatggga cctttgaaga
     4141 acaccctccc ttcgatgtgc cagatgccaa tggcagcttc cttcccagtg cccttggggg
     4201 cttctacatg ttcctcacaa tgatcatcct gctccaggtg ctgatcccca tctctttgta
     4261 tgtctccatt gagctggtga agctcgggca agtgttcttc ttgagcaatg accttgacct
     4321 gtatgatgaa gagaccgatt tatccattca atgtcgagcc ctcaacatcg cagaggactt
     4381 gggccagatc cagtacatct tctccgataa gacggggacc ctgacagaga acaagatggt
     4441 gttccgacgt tgcaccatca tgggcagcga gtattctcac caagaaaatg ctaagcgact
     4501 ggagacccca aaggagctgg actcagatgg tgaagagtgg acccaatacc aatgcctgtc
     4561 cttctcggct agatgggccc aggatccagc aactatgaga agccaaaaag gtgctcagcc
     4621 tctgaggagg agccagagtg cccgggtgcc catccagggc cactaccggc aaaggtctat
     4681 ggggcaccgt gaaagctcac agcctcctgt ggccttcagc agctccatag aaaaagatgt
     4741 aactccagat aaaaacctac tgaccaaggt tcgagatgct gccctgtggt tggagacctt
     4801 gtcagacagc agacctgcca aggcttccct ctccaccacc tcctccattg ctgatttctt
     4861 ccttgcctta accatctgca actctgtcat ggtgtccaca accaccgagc ccaggcagag
     4921 ggtcaccatc aaaccctcaa gcaaggctct ggggacgtcc ctggagaaga ttcagcagct
     4981 cttccagaag ttgaagctat tgagcctcag ccagtcattc tcatccactg caccctctga
     5041 cacagacctc ggggagagct taggggccaa cgtggccacc acagactcgg atgagagaga
     5101 tgatgcatct gtgtgcagtg gaggtgactc cactgatgac ggtggctaca ggagcagcat
     5161 gtgggaccag ggcgacatcc tggagtctgg gtcaggcact tccttggagg aggcattgga
     5221 ggccccagcc acagacctgg ccaggcctga gttctgttac gaggctgaga gccctgatga
     5281 ggccgccctg gtgcacgctg cccatgccta cagcttcaca ctagtgtccc ggacacctga
     5341 gcaggtgact gtgcgcctgc cccagggcac ctgcctcacc ttcagcctcc tctgcaccct
     5401 gggctttgac tctgtcagga agagaatgtc tgtggttgtg aggcacccac tgactggcga
     5461 gattgttgtc tacaccaagg gtgctgactc ggtcatcatg gacctgctgg aagacccagc
     5521 ctgcgtacct gacattaata tggaaaagaa gctgagaaaa atccgagccc ggacccaaaa
     5581 gcatctagac ttgtatgcaa gagatggcct gcgcacacta tgcattgcca agaaggttgt
     5641 aagcgaagag gacttccgga gatgggccag tttccggcgt gaggctgagg catccctcga
     5701 caaccgagat gagcttctca tggaaactgc acagcatctg gagaatcaac tcaccttact
     5761 tggagccact gggatcgaag accggctgca ggaaggagtt ccagatacga ttgccactct
     5821 gcgggaggct gggatccagc tctgggtcct gactggagat aagcaggaga cagcggtcaa
     5881 cattgcccat tcctgcagac tgttaaatca gaccgacact gtttatacca tcaatacaga
     5941 gaatcaggag acctgtgaat ccatcctcaa ttgtgcattg gaagagctaa agcaatttcg
     6001 tgaactacag aagccagacc gcaagctctt tggattccgc ttaccttcca agacaccatc
     6061 catcacctca gaagctgtgg ttccagaagc tggattggtc atcgatggga agacattgaa
     6121 tgccatcttc cagggaaagc tagagaagaa gtttctggaa ttgacccagt attgtcggtc
     6181 cgtcctgtgc tgccgctcca cgccactcca gaagagtatg atagtcaagc tggtgcgaga
     6241 caagttgcgc gtcatgaccc tttccatagg tgatggagca aatgatgtaa gcatgattca
     6301 agctgctgat attggaattg gaatatctgg acaggaaggc atgcaggctg tcatgtccag
     6361 cgactttgcc atcacccgct ttaagcatct caagaagttg ctgctcgtgc atggccactg
     6421 gtgttactcg cgcctggcca ggatggtggt gtactacctc tacaagaacg tgtgctacgt
     6481 caacctgctc ttctggtatc agttcttctg tggtttctcc agctccacca tgattgatta
     6541 ctggcagatg atattcttca atctcttctt tacctccttg cctcctcttg tctttggagt
     6601 ccttgacaaa gacatctctg cagaaacact cctggcattg cctgagctat acaagagtgg
     6661 ccagaactct gagtgctata acctgtcgac tttctggatt tctatggtgg atgcattcta
     6721 ccagagcctc atctgtttct ttatccctta cctggcctat aagggctctg atatagatgt
     6781 ctttaccttt gggacaccaa tcaacaccat ctccctcacc acaatccttt tgcaccaggc
     6841 aatggaaatg aagacatgga ccattttcca cggagtcgtg ctcctcggca gcttcctgat
     6901 gtactttctg gtatccctcc tgtacaatgc cacctgcgtc atctgcaaca gccccaccaa
     6961 tccctattgg gtgatggaag gccagctctc aaaccccact ttctacctcg tctgctttct
     7021 cacaccagtt gttgctcttc tcccaagata ctttttcctg tctctgcaag gaacttgtgg
     7081 gaagtctcta atctcaaaag ctcagaaaat tgacaaactc cccccagaca aaagaaacct
     7141 ggaaatccag agttggagaa gcagacagag gcctgcccct gtccccgaag tggctcgacc
     7201 aactcaccac ccagtgtcat ctatcacagg acaggacttc agtgccagca ccccaaagag
     7261 ctctaaccct cccaagagga agcatgtgga agagtcagta ctccacgaac agagatgtgg
     7321 cacggagtgc atgagggatg actcatgctc aggggactcc tcagctcaac tctcatccgg
     7381 ggagcacctg ctgggaccta acaggataat ggcctactca agaggacaga ctgatatgtg
     7441 ccggtgctca aagaggagca gccatcgccg atcccagagt tcactgacca tctcgagata
     7501 tctagaccca gctttcttgt acaaagtggt tgatctagag ggcccgcggt tcgaactcga
     7561 ggtaccgaat tcgtacccat acgacgtccc agactacgct taattcactc ctcaggtgca
     7621 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
     7681 agatcgatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
     7741 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     7801 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
     7861 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
     7921 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     7981 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     8041 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     8101 cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg
     8161 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     8221 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     8281 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc
     8341 cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     8401 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     8461 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     8521 gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag
     8581 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     8641 gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa
     8701 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     8761 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8821 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8881 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8941 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9001 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9061 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9121 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9181 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9241 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9301 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     9361 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9421 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9481 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     9541 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     9601 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9661 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9721 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9781 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9841 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9901 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9961 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
    10021 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    10081 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    10141 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    10201 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    10261 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10321 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    10381 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    10441 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10501 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca at
//
