LOCUS       sequence-412671-        9688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of ATP11B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9688)
  AUTHORS   Takatsu H, Baba K, Shima T, Umino H, Kato U, Umeda M, Nakayama K, 
            Shin HW
  TITLE     ATP9B, a P4-ATPase (a putative aminophospholipid translocase), 
            localizes to the trans-Golgi network in a CDC50 protein-independent 
            manner.
  JOURNAL   J Biol Chem. 2011 Nov 4;286(44):38159-38167. doi: 
            10.1074/jbc.M111.281006. Epub 2011 Sep 13.
  PUBMED    21914794
REFERENCE   2  (bases 1 to 9688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.281006"; journalName: "J Biol Chem"; date: 
            "2011-11-4- 4"; volume: "286"; issue: "44"; pages: "38159-38167"
COMMENT     Sequence Label: pCAG/ATP11B-HA
FEATURES             Location/Qualifiers
     source          1..9688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             400..1203
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(463..482)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1073..1092
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1210..1258
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     intron          1926..2942
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2866..2888
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2950..2969
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    3038..3062
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6642..6666)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6710..6736
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6824..6843)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6889..6944
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6943..6962)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7305..7321)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7305..7321)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7318..7340)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7329..7345
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7353..7359,7360..7377,7378..7383))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7398..7419
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7478..7673
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7524..7659
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7679..7813
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7716..7735)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7770..7789
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7881..7898)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8052..8640)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8132..8151)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8811..9602,9603..9671))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9434..9453
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9672..9688,1..88))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
       61 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga
      121 gcagtgtggt tttcaagagg aagcaaaaag cctctccacc caggcctgga atgtttccac
      181 ccaatgtcga gcagtgtggt tttgcaagag gaagcaaaaa gcctctccac ccaggcctgg
      241 aatgtttcca cccaatgtcg agcaaacccc gcccagcgtc ttgtcattgg cgaattaatt
      301 cgaacacgca gatgcagtcg gggcggcgcg gtcccaggtc cacttcgcat attaaggtga
      361 cgcgtgtggc ctcgaacacc gagcgaccct gcagccaata tgggatcggc cattgaacaa
      421 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
      481 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
      541 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
      601 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
      661 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
      721 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
      781 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
      841 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
      901 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgatgatctc
      961 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     1021 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     1081 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     1141 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     1201 tgaggggatc ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcgg
     1261 atccgtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     1321 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1381 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     1441 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     1501 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     1561 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagcaggtc
     1621 tcggtgtgaa gctccccttg ctctgctcgc ggtcgccccc cccctcttcc cccccccccc
     1681 cccccccccc ccccctcccc tccctttgtt tttttttttt ttgtttttgg tggggggggg
     1741 gggggggggg gggggggggg gggggggggg gggggcgggg gggggcgggg cggggcgagg
     1801 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
     1861 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
     1921 gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc
     1981 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt
     2041 ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc
     2101 gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg
     2161 cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg
     2221 agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg
     2281 ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg
     2341 gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc
     2401 tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg
     2461 ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg gtgccgggcg
     2521 gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc ccccggagcg
     2581 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
     2641 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
     2701 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     2761 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
     2821 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     2881 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     2941 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaatttgcta
     3001 gcgagctcgc ggccgcagat ctctagttaa gctatcaaca agtttgtaca aaaaagcagg
     3061 ctctttaaag gaaccaattc agtcgactgg atctaccatg tggcgctgga tccggcagca
     3121 gctgggtttt gacccaccac atcagagtga cacaagaacc atctacgtag ccaacaggtt
     3181 tcctcagaat ggcctttaca cacctcagaa atttatagat aacaggatca tttcatctaa
     3241 gtacactgtg tggaattttg ttccaaaaaa tttatttgaa cagttcagaa gagtggcaaa
     3301 cttttatttt cttattatat ttttggttca gcttatgatt gatacaccta ccagtccagt
     3361 taccagtgga cttccattat tctttgtgat aacagtaact gccataaagc agggatatga
     3421 agattggtta cggcataact cagataatga agtaaatgga gctcctgttt atgttgttcg
     3481 aagtggtggc cttgtaaaaa ctagatcaaa aaacattcgg gtgggtgata ttgttcgaat
     3541 agccaaagat gaaatttttc ctgcagactt ggtgcttctg tcctcagatc gactggatgg
     3601 ttcctgtcac gttacaactg ctagtttgga cggagaaact aacctgaaga cacatgtggc
     3661 agttccagaa acagcattat tacaaacagt tgccaatttg gacactctag tagctgtaat
     3721 agaatgccag caaccagaag cagacttata cagattcatg ggacgaatga tcataaccca
     3781 acaaatggaa gaaattgtaa gacctctggg gccggagagt ctcctgcttc gtggagccag
     3841 attaaaaaac acaaaagaaa tttttggtgt tgcggtatac actggaatgg aaactaagat
     3901 ggcattaaat tacaagagca aatcacagaa acgatctgca gtagaaaagt caatgaatac
     3961 atttttgata atttatctag taattcttat atctgaagct gtcatcagca ctatcttgaa
     4021 gtatacatgg caagctgaag aaaaatggga tgaaccttgg tataaccaaa aaacagaaca
     4081 tcaaagaaat agcagtaaga ttctgagatt tatttcagac ttccttgctt ttttggttct
     4141 ctacaatttc atcattccaa tttcattata tgtgacagtc gaaatgcaga aatttcttgg
     4201 atcgtttttt attggctggg atcttgatct gtatcatgaa gaatcagatc agaaagctca
     4261 agtcaatact tccgatctga atgaagagct tggacaggta gagtacgtgt ttacagataa
     4321 aactggtaca ctgacagaaa atgagatgca gtttcgggaa tgttcaatta atggcatgaa
     4381 ataccaagaa attaatggta gacttgtacc cgaaggacca acaccagact cttcagaagg
     4441 aaacttatct tatcttagta gtttatccca tcttaacaac ttatcccatc ttacaaccag
     4501 ttcctctttc agaaccagtc ctgaaaatga aactgaacta attaaagaac atgatctctt
     4561 ctttaaagca gtcagtctct gtcacactgt acagattagc aatgttcaaa ctgactgcac
     4621 tggtgatggt ccctggcaat ccaacctggc accatcgcag ttggagtact atgcatcttc
     4681 accagatgaa aaggctctag tagaagctgc agcaaggatt ggtattgtgt ttattggcaa
     4741 ttctgaagaa actatggagg ttaaaactct tggaaaactg gaacggtaca aactgcttca
     4801 tattctggaa tttgattcag atcgtaggag aatgagtgta attgttcagg caccttcagg
     4861 tgagaagtta ttatttgcta aaggagctga gtcatcaatt ctccctaaat gtataggtgg
     4921 agaaatagaa aaaaccagaa ttcatgtaga tgaatttgct ttgaaagggc taagaactct
     4981 gtgtatagca tatagaaaat ttacatcaaa agagtatgag gaaatagata aacgcatatt
     5041 tgaagccagg actgccttgc agcagcggga agagaaattg gcagctgttt tccagttcat
     5101 agagaaagac ctgatattac ttggagccac agcagtagaa gacagactac aagataaagt
     5161 tcgagaaact attgaagcat tgagaatggc tggtatcaaa gtatgggtac ttactgggga
     5221 taaacatgaa acagctgtta gtgtgagttt atcatgtggc cattttcata gaaccatgaa
     5281 catccttgaa cttataaacc agaaatcaga cagcgagtgt gctgaacaat tgaggcagct
     5341 tgccagaaga attacagagg atcatgtgat tcagcatggg ctggtagtgg atgggaccag
     5401 cctatctctt gcactcaggg agcatgaaaa actatttatg gaagtttgca gaaattgttc
     5461 agctgtatta tgctgtcgta tggctccact gcagaaagca aaagtaataa gactaataaa
     5521 aatatcacct gagaaaccta taacattggc tgttggtgat ggtgctaatg acgtaagcat
     5581 gatacaagaa gcccatgttg gcataggaat catgggtaaa gaaggaagac aggctgcaag
     5641 aaacagtgac tatgcaatag ccagatttaa gttcctctcc aaattgcttt ttgttcatgg
     5701 tcatttttat tatattagaa tagctaccct tgtacagtat tttttttata agaatgtgtg
     5761 ctttatcaca ccccagtttt tatatcagtt ctactgtttg ttttctcagc aaacattgta
     5821 tgacagcgtg tacctgactt tatacaatat ttgttttact tccctaccta ttctgatata
     5881 tagtcttttg gaacagcatg tagaccctca tgtgttacaa aataagccca ccctttatcg
     5941 agacattagt aaaaaccgcc tcttaagtat taaaacattt ctttattgga ccatcctggg
     6001 cttcagtcat gcctttattt tcttttttgg atcctattta ctaataggga aagatacatc
     6061 tctgcttgga aatggccaga tgtttggaaa ctggacattt ggcactttgg tcttcacagt
     6121 catggttatt acagtcacag taaagatggc tctggaaact catttttgga cttggatcaa
     6181 ccatctcgtt acctggggat ctattatatt ttattttgta ttttccttgt tttatggagg
     6241 gattctctgg ccatttttgg gctcccagaa tatgtatttt gtgtttattc agctcctgtc
     6301 aagtggttct gcttggtttg ccataatcct catggttgtt acatgtctat ttcttgatat
     6361 cataaagaag gtctttgacc gacacctcca ccctacaagt actgaaaagg cacagcttac
     6421 tgaaacaaat gcaggtatca agtgcttgga ctccatgtgc tgtttcccgg aaggagaagc
     6481 agcgtgtgca tctgttggaa gaatgctgga acgagttata ggaagatgta gtccaaccca
     6541 catcagcaga tcatggagtg catcggatcc tttctatacc aacgacagga gcatcttgac
     6601 tctctccaca atggactcat ctacttgctc gagatatcta gacccagctt tcttgtacaa
     6661 agtggttgat ctagagggcc cgcggttcga actcgaggta ccgaattcgt acccatacga
     6721 cgtcccagac tacgcttaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc
     6781 tggtgtggcc aatgccctgg ctcacaaata ccactgagat cgatcttttt ccctctgcca
     6841 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
     6901 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
     6961 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
     7021 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac
     7081 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt
     7141 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt
     7201 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct
     7261 tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct
     7321 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7381 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7441 gctttccagt cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca
     7501 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     7561 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     7621 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa
     7681 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7741 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7801 tcatgtctgg atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7861 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7921 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7981 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8041 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     8101 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     8161 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     8221 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8281 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8341 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8401 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8461 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     8521 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8581 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8641 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8701 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8761 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8821 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8881 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8941 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9001 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9061 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     9121 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     9181 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9241 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9301 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9361 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9421 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9481 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9541 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9601 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9661 tgaatactca tactcttcct ttttcaat
//
