LOCUS       sequence-412673-         450 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses truncated BID upon doxycycline addition to trigger 
            apoptosis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Nam M, Xia W, Mir AH, Jerrett A, Spinelli JB, Huang TT, Possemato R
  TITLE     Glucose limitation protects cancer cells from apoptosis induced by 
            pyrimidine restriction and replication inhibition.
  JOURNAL   Nat Metab. 2024 Dec;6(12):2338-2353. doi: 
            10.1038/s42255-024-01166-w. Epub 2024 Nov 26.
  PUBMED    39592843
REFERENCE   2  (bases 1 to 450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s42255-024-01166-w"; journalName: "Nat Metab"; date: 
            "2024-12"; volume: "6"; issue: "12"; pages: "2338-2353"
COMMENT     Sequence Label: pCW57.1 blast tBID-flag
FEATURES             Location/Qualifiers
     source          1..450
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             424..447
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 accatgggca accgcagcag ccactcccgc ttgggaagaa tagaggcaga ttctgaaagt
       61 caagaagaca tcatccggaa tattgccagg cacctcgccc aggtcgggga cagcatggac
      121 cgtagcatcc ctccgggcct ggtgaacggc ctggccctgc agctcaggaa caccagccgg
      181 tcggaggagg accggaacag ggacctggcc actgccctgg agcagctgct gcaggcctac
      241 cctagagaca tggagaagga gaagaccatg ctggtgctgg ccctgctgct ggccaagaag
      301 gtggccagtc acacgccgtc cttgctccgt gatgtctttc acacaacagt gaattttatt
      361 aaccagaacc tacgcaccta cgtgaggagc ttagccagaa atgggatgga cgccggagga
      421 gccgattaca aggacgacga tgacaagtga
//
