LOCUS       sequence-412677-        1473 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses flag-tagged cFLIP (CFLAR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Nam M, Xia W, Mir AH, Jerrett A, Spinelli JB, Huang TT, Possemato R
  TITLE     Glucose limitation protects cancer cells from apoptosis induced by 
            pyrimidine restriction and replication inhibition.
  JOURNAL   Nat Metab. 2024 Dec;6(12):2338-2353. doi: 
            10.1038/s42255-024-01166-w. Epub 2024 Nov 26.
  PUBMED    39592843
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s42255-024-01166-w"; journalName: "Nat Metab"; date: 
            "2024-12"; volume: "6"; issue: "12"; pages: "2338-2353"
COMMENT     Sequence Label: pLJM1 flag-cFLIP
FEATURES             Location/Qualifiers
     source          1..1473
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..30
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 accatggact acaaagacga tgacgacaag atgtctgctg aagtcatcca tcaggttgaa
       61 gaagcacttg atacagatga gaaggagatg ctgctctttt tgtgccggga tgttgctata
      121 gatgtggttc cacctaatgt cagggacctt ctggatattt tacgggaaag aggtaagctg
      181 tctgtcgggg acttggctga actgctctac agagtgaggc gatttgacct gctcaaacgt
      241 atcttgaaga tggacagaaa agctgtggag acccacctgc tcaggaaccc tcaccttgtt
      301 tcggactata gagtgctgat ggcagagatt ggtgaggatt tggataaatc tgatgtgtcc
      361 tcattaattt tcctcatgaa ggattacatg ggccgaggca agataagcaa ggagaagagt
      421 ttcttggacc ttgtggttga gttggagaaa ctaaatctgg ttgccccaga tcaactggat
      481 ttattagaaa aatgcctaaa gaacatccac agaatagacc tgaagacaaa aatccagaag
      541 tacaagcagt ctgttcaagg agcagggaca agttacagga atgttctcca agcagcaatc
      601 caaaagagtc tcaaggatcc ttcaaataac ttcaggctcc ataatgggag aagtaaagaa
      661 caaagactta aggaacagct tggcgctcaa caagaaccag tgaagaaatc cattcaggaa
      721 tcagaagctt ttttgcctca gagcatacct gaagagagat acaagatgaa gagcaagccc
      781 ctaggaatct gcctgataat cgattgcatt ggcaatgaga cagagcttct tcgagacacc
      841 ttcacttccc tgggctatga agtccagaaa ttcttgcatc tcagtatgca tggtatatcc
      901 cagattcttg gccaatttgc ctgtatgccc gagcaccgag actacgacag ctttgtgtgt
      961 gtcctggtga gccgaggagg ctcccagagt gtgtatggtg tggatcagac tcactcaggg
     1021 ctccccctgc atcacatcag gaggatgttc atgggagatt catgccctta tctagcaggg
     1081 aagccaaaga tgttttttat tcagaactat gtggtgtcag agggccagct ggaggacagc
     1141 agcctcttgg aggtggatgg gccagcgatg aagaatgtgg aattcaaggc tcagaagcga
     1201 gggctgtgca cagttcaccg agaagctgac ttcttctgga gcctgtgtac tgcggacatg
     1261 tccctgctgg agcagtctca cagctcacca tccctgtacc tgcagtgcct ctcccagaaa
     1321 ctgagacaag aaagaaaacg cccactcctg gatcttcaca ttgaactcaa tggctacatg
     1381 tatgattgga acagcagagt ttctgccaag gagaaatatt atgtctggct gcagcacact
     1441 ctgagaaaga aacttatcct ctcctacaca taa
//
