LOCUS       sequence-41268-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Garcia DM, Baek D, Shin C, Bell GW, Grimson A, Bartel DP
  TITLE     Weak seed-pairing stability and high target-site abundance decrease 
            the proficiency of lsy-6 and other microRNAs.
  JOURNAL   Nat Struct Mol Biol. 2011 Sep 11;18(10):1139-46. doi: 
            10.1038/nsmb.2115.
  PUBMED    21909094
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb"; 
            journalName: "Nat Struct Mol Biol"; date: "2011-09-11- 11"; volume: 
            "18"; issue: "10"; pages: "1139-46"
COMMENT     Sequence Label: pCS260
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    707..828
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(718..737)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     772..791
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctgaatttgt caaagtaaaa ggtcttcatt tttcgcaaga agatgcacct gatgaaatgg
       61 gaaaatatat caaatcgttc gttgagcgag ttctcaaaaa tgaacaataa ttctaggagc
      121 tccagacaga gatgtgctga ttttgtttta gctgtaacag gtaatggttt ttggatagat
      181 gattgactgg tgagaatttg gtcaaggtga cagcctcctg tctgatgaca ggacagactg
      241 gtggtgagga gtctaagtgg gctcagtttg atgtcagtgt ctgggctcat gacttgtaaa
      301 tggaagctga cgcgaacagg taattaatat tatgacccac ttctatttac tttgggaaat
      361 atcttggatc ttaattatca tctgcaagtt tcaagaagta ttctgccaaa agtatttaca
      421 agtatggact catgagctat tgttggttgc taaacgcgaa tcacgcggga gtgagtgtgc
      481 ccttcacact gtgacattgt gacattgtga caagctccat gtcctttaaa atcagtcact
      541 ctgcacacaa gagaaatcaa cttcgtggtt ggatggggcc ggaacacaac cagtcttttt
      601 gtatttattg ttactgagac aaaacagtac tcactgagtg tttttcagtt tcctactggt
      661 ggttttgaac tagttgttct agagcggccg cttcgagcag acatgataag atacattgat
      721 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
      781 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caccacaatt
      841 gcattcattt atgtttcagg tc
//
