LOCUS       sequence-412680-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty Gateway pDEST vector for bacterial expression with Super His -
            Monomeric OCR fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Szabla R, Li M, Warner V, Song Y, Junop M
  TITLE     DdrC, a unique DNA repair factor from D. radiodurans, senses and 
            stabilizes DNA breaks through a novel lesion-recognition mechanism.
  JOURNAL   Nucleic Acids Res. 2024 Jul 22:gkae635. doi: 10.1093/nar/gkae635.
  PUBMED    39036966
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2024 Jul 22:gkae635. doi: 10.1093/nar/gkae635."
COMMENT     Sequence Label: pDEST-SHmOCR
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    506..630
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(655..660,661..678,679..685)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(805..824)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgagatct aagcaccacc accacagcgg
       61 tcatcatcac accggccatc atcaccacag cggtagccac caccacagcg gtaccggtag
      121 cagcggcgtg gacctggcga tgagcaacat gacctacaac aacgttttcg atcacgcgta
      181 tgagatgctg aaagaaaaca tccgttacga cgatattcgt gacaccgacg atctgcacga
      241 tgcgattcat atggcggcgg acaacgcggt gccgcactac tatgcggata tccgtagcgt
      301 tatggcgagc gagggtattg acctggagtt tgaagatagc ggcctgatgc cggacaccaa
      361 ggacgatatc cgtattctgc aggcgcgtat ctatgagcaa ctgaccattg atctgtggga
      421 ggacgcggag gacctgctga acgaatacct ggaggaagtg gaggaatatg aggaagacga
      481 ggaaggtacc ggtagatctg atatcacaag tttgtacaaa aaagctgaac gagaaacgta
      541 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
      601 ctgtaaaaca caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca
      661 ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca
      721 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc
      781 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac
      841 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaa
//
