LOCUS       sequence-412684-        6055 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Nishio H, Walsh MJ
  TITLE     CCAAT displacement protein/cut homolog recruits G9a histone lysine 
            methyltransferase to repress transcription.
  JOURNAL   Proc Natl Acad Sci U S A. 2004 Aug 3;101(31):11257-62. Epub 2004 Jul
            21.
  PUBMED    15269344
REFERENCE   2  (bases 1 to 6055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2004-08-3"; volume: "101(31)"; pages: 
            "11257-62. Epub 2004 Jul 21"
COMMENT     Sequence Label: pGL2-GAL4-UAS-Luc
FEATURES             Location/Qualifiers
     source          1..6055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     252..276
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             553..2205
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(619..637)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          2446..2511
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2641..2661
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3086..3220
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3362..3379)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3533..4121)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3613..3632)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4292..5083,5084..5152))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4915..4934
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5153..5257)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5284..5739
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5371..5390)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5581..5602
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5929..6050
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ctgtaactga gctaacataa cccgggaggt accgagctct tacgcgtgct agctcgagat
       61 ctccgagctc ttacgcgggt cgaagcggag tactgtcctc cgagtggagt actgtcctcc
      121 gagcggagta ctgtcctccg agtcgagggt cgaagcggag tactgtcctc cgagtggagt
      181 actgtcctcc gagcggagta ctgtcctccg agtcgactct agagggtata taatggatct
      241 cgagatatcg gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt
      301 tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccggga acggtgcatt
      361 ggaacgcgca ttccccgtgt taattaacag gtaagtgtct tcctcctgtt tccttcccct
      421 gctattctgc tcaaccttcc tatcagaaac tgcagtatct gtatttttgc tagcagtaat
      481 actaacggtt ctttttttct cttcacaggc cacagatcta agtaagcttg gcattccggt
      541 actgttggta aaatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatcct
      601 ctagaggatg gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt
      661 cctggaacaa ttgcttttac agatgcacat atcgaggtga acatcacgta cgcggaatac
      721 ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac
      781 agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta
      841 tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac
      901 agtatgaaca tttcgcagcc taccgtagtg tttgtttcca aaaaggggtt gcaaaaaatt
      961 ttgaacgtgc aaaaaaaatt accaataatc cagaaaatta ttatcatgga ttctaaaacg
     1021 gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt
     1081 aatgaatacg attttgtacc agagtccttt gatcgtgaca aaacaattgc actgataatg
     1141 aattcctctg gatctactgg gttacctaag ggtgtggccc ttccgcatag aactgcctgc
     1201 gtcagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg
     1261 attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg
     1321 atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tttacgatcc
     1381 cttcaggatt acaaaattca aagtgcgttg ctagtaccaa ccctattttc attcttcgcc
     1441 aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc ttctgggggc
     1501 gcacctcttt cgaaagaagt cggggaagcg gttgcaaaac gcttccatct tccagggata
     1561 cgacaaggat atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat
     1621 gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg
     1681 gataccggga aaacgctggg cgttaatcag agaggcgaat tatgtgtcag aggacctatg
     1741 attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga
     1801 tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt catagttgac
     1861 cgcttgaagt ctttaattaa atacaaagga tatcaggtgg cccccgctga attggaatcg
     1921 atattgttac aacaccccaa catcttcgac gcgggcgtgg caggtcttcc cgacgatgac
     1981 gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa
     2041 gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt
     2101 gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga
     2161 gagatcctca taaaggccaa gaagggcgga aagtccaaat tgtaaaatgt aactgtattc
     2221 agcgatgacg aaattcttag ctattgtaat actgcgatga gtggcagggc ggggcgtaat
     2281 ttttttaagg cagttattgg tgcccttaaa cgcctggttg ctacgcctga ataagtgata
     2341 ataagcggat gaatggcaga aattcgccgg atctttgtga aggaacctta cttctgtggt
     2401 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
     2461 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
     2521 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc
     2581 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct
     2641 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt
     2701 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag
     2761 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt
     2821 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg
     2881 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg
     2941 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
     3001 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     3061 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     3121 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     3181 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgtcgaccga tgcccttgag
     3241 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
     3301 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt
     3361 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     3421 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     3481 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     3541 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     3601 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     3661 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3721 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3781 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3841 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3901 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3961 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     4021 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     4081 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     4141 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     4201 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     4261 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     4321 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     4381 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     4441 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     4501 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     4561 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     4621 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     4681 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     4741 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     4801 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     4861 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     4921 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     4981 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     5041 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     5101 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     5161 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     5221 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     5281 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5341 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     5401 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     5461 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     5521 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     5581 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     5641 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     5701 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg ccattcgcca ttcaggctgc
     5761 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag cccaagctac
     5821 catgataagt aagtaatatt aaggtacgtg gaggttttac ttgctttaaa aaacctccca
     5881 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     5941 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6001 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tggta
//
