LOCUS       sequence-412696-        9183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of evoCjCas9 eAID.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9183)
  AUTHORS   Schmidheini L, Mathis N, Marquart KF, Rothgangl T, Kissling L, Bock 
            D, Chanez C, Wang JP, Jinek M, Schwank G
  TITLE     Continuous directed evolution of a compact CjCas9 variant with broad
            PAM compatibility.
  JOURNAL   Nat Chem Biol. 2023 Sep 21. doi: 10.1038/s41589-023-01427-x.
  PUBMED    37735239
REFERENCE   2  (bases 1 to 9183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2023 Sep 21. doi: 10.1038/s41589-023-01427-x."
COMMENT     Sequence Label: p2T-CMV-evoCjCas9-eAID-BlastR
FEATURES             Location/Qualifiers
     source          1..9183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             116..136
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3758..4006
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             4037..4285
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             4328..4348
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4358..4375
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4398..4415)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4404..4628
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4699..4715)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4699..4715)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4712..4734)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4723..4739
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4747..4753,4754..4771,4772..4777))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4792..4813
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4930..5051
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4941..4960)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4995..5014
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5209..5607)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(5626..5673)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(5721..6050)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5735..5870
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5789..5808)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     6035..6055
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    join(6174..6361,6362..6373)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     rep_origin      complement(6434..7022)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6514..6533)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7193..7984,7985..8053))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7816..7835
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8054..8158)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8237..8256)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(8448..8459,8460..8597)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     enhancer        8606..8985
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(8986..9183,1..6)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9140..9160
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agagctggtt tagtgaaccg tcagatccgc tagagatccg cggccgctaa tacgactcac
       61 tatagggaga gccgccacca tgaaacggac agccgacgga agcgagttcg agtcaccaaa
      121 gaagaagcgg aaagtcagtg atagcctctt gatgaataga cgcgaattcc tgtatcagtt
      181 taaaaacgtg agatgggcaa aaggccgacg agagacatat ctgtgctatg tcgttaagcg
      241 cagagattca gccaccagtt tctctctcga cttcggctac ctgcggaaca agaatggttg
      301 ccatgttgag ctcctgttcc tgaggtatat cagcgactgg gatttggacc cagggcggtg
      361 ctatagggtg acatggttta tttcctggtc accttgttat gactgcgcgc ggcatgttgc
      421 cgattttctg agagggaacc ctaacctgtc tctgaggatc ttcaccgcgc gactgtactt
      481 ctgtgaggac cggaaagccg aacccgaggg actgagacgc ctccacagag cgggtgtgca
      541 gattgccata atgaccttta aggactactt ctactgctgg aacaccttcg tcgaaaatca
      601 cggccggact ttcaaggctt gggaaggatt gcatgaaaac agcgtcaggc tttccaggca
      661 gcttcgccgc attcttctcc cgtctggcgg atctagcgga ggatcctctg gcagcgagac
      721 accaggaaca agcgagtcag caacaccaga gagcagtggc ggcagcagcg gcgggtcagc
      781 ccgcatcctg gcctttgcca tcggcatcag cagcatcgga tgggccttca gcgagaatga
      841 cgagctgaag gactgcggtg tgagaatctt tacaaaggtc gagaacccca agaccggcga
      901 aagcctggcg ctgccaagac ggctggcccg gtctgctcgg aagagatacg cccggagaaa
      961 agccagactg aatcacctga aacacctgat cgccaacgag ttcaaactga actacgagga
     1021 ctaccagagc ttcgatgaga gcttggctaa ggcctacaaa ggcagcctga tcagccccta
     1081 cgagctgaga ttcagagctc tgaatgagct cctgagcaag caggacttcg ctagagtgat
     1141 cctgcacatc gccaagcgaa gaggctacga cgacatcaag aactctgatg acaaggagaa
     1201 gggcgccatt ctgaaagcca tcaagcagaa cgaagagaaa ctggccaatt accagagcgt
     1261 gggcgagtac ctgtacaagg agtacttcca gaagttcaaa gaaaatagca aagagttcac
     1321 caacgtgcgg aacaagaagg agtcttatga aagatgtatc gcccagagct tcctgaaaga
     1381 cgagctgaaa ctgatcttca agaagcaaag agagtttggc ttcagcttca gcaaaaaatt
     1441 tgaagaagag gtgctgtctg tcgccttcta caagagggct ctgaaggact tcagccacct
     1501 ggtgggcaat tgcagctttt tcacagacga aaagcgggcc cctaagaaca gccctctggc
     1561 cttcatgttc gtggctctga ccagaatcat caacctgctg aacaacctga aaaataccga
     1621 gggcatcctc tataccaagg acgatctgaa cgccctgctg aatgaggtgc tcaagaatgg
     1681 caccctgacc tacaagcaaa caaaaaaact gctgggcctg tctgacgact acgagtttaa
     1741 aggcgagaag ggcacctatt tcatcgaatt taagaagtac aaggaattca tcaaggcact
     1801 gggcgaacac aacctgtctc aggacgacct gaacgagatc gccaaggaca tcaccctgat
     1861 caaggatgag atcaagctga aaaaagctct ggccaagtac gacctgaatc aaaaccagat
     1921 cgacagctta agcaagctgg aatttaagga tcacctgaac atctccttta aggccctgaa
     1981 gctggtgacc ccactgatgc tggaaggaaa gaagtacgac gaagcatgca acgagcttaa
     2041 cctgaaggtt gctatcaacg aggataagaa ggatttcctg cctgccttta acgagacata
     2101 ctacaaggat gaggtgacca accccgtggt gctgagagct atcaaagagt acagaaaggt
     2161 gctgaacgcc ctgctgaaga agtacggcaa ggtccacaag atcaatattg agctggcccg
     2221 ggaggttgga aagaaccact ctcagagagc aaagatcgag aaggagcaaa acgagaacta
     2281 caaagcgaag aaggacgccg aactggagtg cgagaagctt ggcctgaaga tcaactctaa
     2341 gaatatcctg aaactcagac ttttcaaaga acagaaggaa ttctgtgcct acagcggcga
     2401 gaagatcaaa atttctgacc tgcaggatga aaagatgctg gagatcgacc acatctaccc
     2461 ttactccaga agcttcgacg acagctatat gaacaaagtg ctggtgttca caaagcagaa
     2521 ccaggagaag ctgaatcaga cccctttcga ggccttcggc aatgactccg ccaagtggca
     2581 gaaaatcgag gtgctggcca aaaacctgcc aaccaagaaa cagaagagaa tcctcgacaa
     2641 gaactacaag gacaaggaac agaagaactt caaggatcgg aacctgaacg acacccggta
     2701 catcgccagg ctggtgttaa attacaccaa ggactacctg gatttcctgc ccctgagcga
     2761 cgacgagaac accaagctga acgacacaca gaagggcagc aaggtgcacg tggaagccaa
     2821 gagcggcatg ctgaccagcg cactgcgcca cacttggggc ttcagcgcta aggaccggaa
     2881 caaccacctg catcacgcca tcgatgccgt gatcatagcc tacgccaaca actcaatcgt
     2941 gaaagctttc agtgacttta agaaagaaca ggagagcaac tctgccgaac tgtacgccaa
     3001 gaaaattagc gagctggact acaagaacaa acggaagttc ttcgaacctt tctcaggatt
     3061 tagacagaag gtgctggata agatcgatga aatcttcgtg tccaagcccg agagaaagaa
     3121 gcctagcgga gccctgcaca aggaaacctt cagaaaggaa gaagagttct accagtctta
     3181 tgggggcaaa gaaggcgtgc tgaaggccct ggaactcggc aagatcagaa aggtgaaggg
     3241 aaagattgtg aagaacggcg acatgttcag agtggacatc ttcaagcaca agaagaccaa
     3301 caagttctat gccgtgccta tctacacaat ggatttcgcc ctgaaagtgc tgcctaacaa
     3361 ggctgtcgcc agatctaaga agggcgagat caaggactgg atcctgatgg acgaaaacta
     3421 cgagttctgc ttcagcctgt acaaggacag cctgatcctg atccaaacaa agaagatgca
     3481 ggagcctgaa ttcgtgtact acaacgcctt cacaagcagc accgtgagcc tgatcgtgtc
     3541 taaacatgat aacaagttcg aaaccctgtc caagaaccaa aagatcctgt tcaagaacgc
     3601 caacgagaag gaagtgatcg ccaagggcat cggcattcag aatctgaagg tgttcgagaa
     3661 atatatcgtg tccgctctgg gagaggttac aaaggccgag tttcggcaga gagaagattt
     3721 taagaagagc ggcgggagcg gcgggagcgg ggggagcact aatctgagcg acatcattga
     3781 gaaggagact gggaaacagc tggtcattca ggagtccatc ctgatgctgc ctgaggaggt
     3841 ggaggaagtg atcggcaaca agccagagtc tgacatcctg gtgcacaccg cctacgacga
     3901 gtccacagat gagaatgtga tgctgctgac ctctgacgcc cccgagtata agccttgggc
     3961 cctggtcatc caggattcta acggcgagaa taagatcaag atgctgagcg gaggatccgg
     4021 aggatctgga ggcagcacca acctgtctga catcatcgag aaggagacag gcaagcagct
     4081 ggtcatccag gagagcatcc tgatgctgcc cgaagaagtc gaagaagtga tcggaaacaa
     4141 gcctgagagc gatatcctgg tccataccgc ctacgacgag agtaccgacg aaaatgtgat
     4201 gctgctgaca tccgacgccc cagagtataa gccctgggct ctggtcatcc aggattccaa
     4261 cggagagaac aaaatcaaaa tgctgtctgg cggctcaaaa agaaccgccg acggcagcga
     4321 attcgagccc aagaagaaga ggaaagtcta aaccggtcat catcaccatc accattgagt
     4381 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     4441 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     4501 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     4561 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     4621 ctctatggct tctgaggcgg aaagaaccag ctggggctcg ataccgtcga cctctagcta
     4681 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     4741 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tagggtgcct aatgagtgag
     4801 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     4861 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcta
     4921 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     4981 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     5041 ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg gaggatcatc
     5101 cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc ggcggtggaa
     5161 tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca cgaagtgctt agccctccca
     5221 cacataacca gagggcagca attcacgaat cccaactgcc gtcggctgtc catcactgtc
     5281 cttcactatg gctttgatcc caggatgcag atcgagaagc acctgtcggc accgtccgca
     5341 ggggctcaag atgcccctgt tctcatttcc gatcgcgacg atacaagtca ggttgccagc
     5401 tgccgcagca gcagcagtgc ccagcaccac gagttctgca caaggtcccc cagtaaaatg
     5461 atatacattg acaccagtga agatgcggcc gtcgctagag agagctgcgc tggcgacgct
     5521 gtagtcttca gagatgggga tgctgttgat tgtagccgtt gctctttcaa tgagggtgga
     5581 ttcttcttga gacaaaggct tggccatggt ttagttcctc accttgtcgt attatactat
     5641 gccgatatac tatgccgatg attaattgtc aacacgtgct gatcagatcc gaaaatggat
     5701 atacaagctc ccgggagctt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac
     5761 ttctggaata gctcagaggc agaggcggcc tcggcctctg cataaataaa aaaaattagt
     5821 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt
     5881 aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     5941 ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata
     6001 cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac attccacaga
     6061 attcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6121 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtaagtgat
     6181 ctccaaaaaa taagtacttt ttgactgtaa ataaaattgt aaggagtaaa aagtactttt
     6241 ttttctaaaa aaatgtaatt aagtaaaagt aaaagtattg atttttaatt gtactcaagt
     6301 aaagtaaaaa tccccaaaaa taatacttaa gtacagtaat caagtaaaat tactcaagta
     6361 ctttacacct ctgcatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6421 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6481 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6541 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6601 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6661 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6721 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6781 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6841 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     6901 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6961 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7021 aagaagatcc tttgatcttt tctacggggt ctgacactca gtggaacgaa aactcacgtt
     7081 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7141 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7201 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7261 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7321 caatgatacc gcgggaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7381 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7441 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7501 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7561 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7621 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     7681 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7741 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7801 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7861 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7921 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7981 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     8041 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     8101 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     8161 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatcgatct cccgatcccc
     8221 tagggtcgac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
     8281 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
     8341 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
     8401 gcgatgtacg ggccagatat acgcgggtac cggcatatgg ttcttgacag aggtgtaaaa
     8461 agtactcaaa aattttactc aagtgaaagt acaagtactt agggaaaatt ttactcaatt
     8521 aaaagtaaaa gtatctggct agaatcttac ttgagtaaaa gtaaaaaagt actccattaa
     8581 aattgtactt gagtattaac gcgttgacat tgattattga ctagttatta atagtaatca
     8641 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     8701 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     8761 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     8821 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     8881 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     8941 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     9001 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     9061 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     9121 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     9181 agc
//
