LOCUS       sequence-412697-        9569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of evoCjCas9 PEmax_deltaRnH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9569)
  AUTHORS   Schmidheini L, Mathis N, Marquart KF, Rothgangl T, Kissling L, Bock 
            D, Chanez C, Wang JP, Jinek M, Schwank G
  TITLE     Continuous directed evolution of a compact CjCas9 variant with broad
            PAM compatibility.
  JOURNAL   Nat Chem Biol. 2023 Sep 21. doi: 10.1038/s41589-023-01427-x.
  PUBMED    37735239
REFERENCE   2  (bases 1 to 9569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2023 Sep 21. doi: 10.1038/s41589-023-01427-x."
COMMENT     Sequence Label: p2T-CMV-evoCjCas9-PEmax_dRnH-BlastR
FEATURES             Location/Qualifiers
     source          1..9569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             116..136
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3149..3169
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4679..4699
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4709..4735
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             4744..4761
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4784..4801)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4790..5014
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5085..5101)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5085..5101)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5098..5120)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5109..5125
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5133..5139,5140..5157,5158..5163))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5178..5199
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    5316..5437
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5327..5346)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5381..5400
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(5595..5993)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(6012..6059)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(6107..6436)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6121..6256
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6175..6194)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     6421..6441
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    join(6560..6747,6748..6759)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     rep_origin      complement(6820..7408)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6900..6919)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7579..8370,8371..8439))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8202..8221
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8440..8544)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8623..8642)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(8834..8845,8846..8983)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     enhancer        8992..9371
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(9372..9569,1..6)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9526..9546
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 agagctggtt tagtgaaccg tcagatccgc tagagatccg cggccgctaa tacgactcac
       61 tatagggaga gccgccacca tgaaacggac agccgacgga agcgagttcg agtcaccaaa
      121 gaagaagcgg aaagtcgggt cagcccgcat cctggccttt gacatcggca tcagcagcat
      181 cggatgggcc ttcagcgaga atgacgagct gaaggactgc ggtgtgagaa tctttacaaa
      241 ggtcgagaac cccaagaccg gcgaaagcct ggcgctgcca agacggctgg cccggtctgc
      301 tcggaagaga tacgcccgga gaaaagccag actgaatcac ctgaaacacc tgatcgccaa
      361 cgagttcaaa ctgaactacg aggactacca gagcttcgat gagagcttgg ctaaggccta
      421 caaaggcagc ctgatcagcc cctacgagct gagattcaga gctctgaatg agctcctgag
      481 caagcaggac ttcgctagag tgatcctgca catcgccaag cgaagaggct acgacgacat
      541 caagaactct gatgacaagg agaagggcgc cattctgaaa gccatcaagc agaacgaaga
      601 gaaactggcc aattaccaga gcgtgggcga gtacctgtac aaggagtact tccagaagtt
      661 caaagaaaat agcaaagagt tcaccaacgt gcggaacaag aaggagtctt atgaaagatg
      721 tatcgcccag agcttcctga aagacgagct gaaactgatc ttcaagaagc aaagagagtt
      781 tggcttcagc ttcagcaaaa aatttgaaga agaggtgctg tctgtcgcct tctacaagag
      841 ggctctgaag gacttcagcc acctggtggg caattgcagc tttttcacag acgaaaagcg
      901 ggcccctaag aacagccctc tggccttcat gttcgtggct ctgaccagaa tcatcaacct
      961 gctgaacaac ctgaaaaata ccgagggcat cctctatacc aaggacgatc tgaacgccct
     1021 gctgaatgag gtgctcaaga atggcaccct gacctacaag caaacaaaaa aactgctggg
     1081 cctgtctgac gactacgagt ttaaaggcga gaagggcacc tatttcatcg aatttaagaa
     1141 gtacaaggaa ttcatcaagg cactgggcga acacaacctg tctcaggacg acctgaacga
     1201 gatcgccaag gacatcaccc tgatcaagga tgagatcaag ctgaaaaaag ctctggccaa
     1261 gtacgacctg aatcaaaacc agatcgacag cttaagcaag ctggaattta aggatcacct
     1321 gaacatctcc tttaaggccc tgaagctggt gaccccactg atgctggaag gaaagaagta
     1381 cgacgaagca tgcaacgagc ttaacctgaa ggttgctatc aacgaggata agaaggattt
     1441 cctgcctgcc tttaacgaga catactacaa ggatgaggtg accaaccccg tggtgctgag
     1501 agctatcaaa gagtacagaa aggtgctgaa cgccctgctg aagaagtacg gcaaggtcca
     1561 caagatcaat attgagctgg cccgggaggt tggaaagaac cactctcaga gagcaaagat
     1621 cgagaaggag caaaacgaga actacaaagc gaagaaggac gccgaactgg agtgcgagaa
     1681 gcttggcctg aagatcaact ctaagaatat cctgaaactc agacttttca aagaacagaa
     1741 ggaattctgt gcctacagcg gcgagaagat caaaatttct gacctgcagg atgaaaagat
     1801 gctggagatc gacgccatct acccttactc cagaagcttc gacgacagct atatgaacaa
     1861 agtgctggtg ttcacaaagc agaaccagga gaagctgaat cagacccctt tcgaggcctt
     1921 cggcaatgac tccgccaagt ggcagaaaat cgaggtgctg gccaaaaacc tgccaaccaa
     1981 gaaacagaag agaatcctcg acaagaacta caaggacaag gaacagaaga acttcaagga
     2041 tcggaacctg aacgacaccc ggtacatcgc caggctggtg ttaaattaca ccaaggacta
     2101 cctggatttc ctgcccctga gcgacgacga gaacaccaag ctgaacgaca cacagaaggg
     2161 cagcaaggtg cacgtggaag ccaagagcgg catgctgacc agcgcactgc gccacacttg
     2221 gggcttcagc gctaaggacc ggaacaacca cctgcatcac gccatcgatg ccgtgatcat
     2281 agcctacgcc aacaactcaa tcgtgaaagc tttcagtgac tttaagaaag aacaggagag
     2341 caactctgcc gaactgtacg ccaagaaaat tagcgagctg gactacaaga acaaacggaa
     2401 gttcttcgaa cctttctcag gatttagaca gaaggtgctg gataagatcg atgaaatctt
     2461 cgtgtccaag cccgagagaa agaagcctag cggagccctg cacaaggaaa ccttcagaaa
     2521 ggaagaagag ttctaccagt cttatggggg caaagaaggc gtgctgaagg ccctggaact
     2581 cggcaagatc agaaaggtga agggaaagat tgtgaagaac ggcgacatgt tcagagtgga
     2641 catcttcaag cacaagaaga ccaacaagtt ctatgccgtg cctatctaca caatggattt
     2701 cgccctgaaa gtgctgccta acaaggctgt cgccagatct aagaagggcg agatcaagga
     2761 ctggatcctg atggacgaaa actacgagtt ctgcttcagc ctgtacaagg acagcctgat
     2821 cctgatccaa acaaagaaga tgcaggagcc tgaattcgtg tactacaacg ccttcacaag
     2881 cagcaccgtg agcctgatcg tgtctaaaca tgataacaag ttcgaaaccc tgtccaagaa
     2941 ccaaaagatc ctgttcaaga acgccaacga gaaggaagtg atcgccaagg gcatcggcat
     3001 tcagaatctg aaggtgttcg agaaatatat cgtgtccgct ctgggagagg ttacaaaggc
     3061 cgagtttcgg cagagagaag attttaagaa gtccggcgga agctctggtg gcagcaagcg
     3121 gaccgccgac ggctctgaat tcgagagccc taagaagaaa agaaaggtga gcggaggctc
     3181 tagcggcgga agcaccctga acattgaaga cgagtataga ctgcatgaaa caagcaagga
     3241 acccgacgtg tccctgggct ccacctggct gtccgacttt ccccaggcct gggccgagac
     3301 aggaggaatg ggcctggccg tgcggcaggc acccctgatc atccctctga aggccacctc
     3361 tacacccgtg agcatcaagc agtaccctat gtctcaggag gccagactgg gcatcaagcc
     3421 tcacatccag aggctgctgg accagggcat cctggtgcca tgccagagcc cctggaacac
     3481 accactgctg cccgtgaaga agccaggcac caatgactat agacccgtgc aggatctgag
     3541 agaggtgaac aagagggtgg aggatatcca ccccaccgtg cccaaccctt acaatctgct
     3601 gtccggcctg cccccttctc accagtggta tacagtgctg gacctgaagg atgccttctt
     3661 ttgtctgaga ctgcacccta ccagccagcc actgttcgcc tttgagtgga gggaccctga
     3721 gatgggcatc tctggccagc tgacctggac acgcctgcct cagggcttca agaatagccc
     3781 aacactgttt aacgaggccc tgcaccgcga cctggcagat ttccggatcc agcacccaga
     3841 tctgatcctg ctgcagtacg tggacgatct gctgctggcc gccaccagcg agctggattg
     3901 ccagcaggga acacgcgccc tgctgcagac cctgggaaac ctgggatata gggcatccgc
     3961 caagaaggcc cagatctgtc agaagcaggt gaagtacctg ggctatctgc tgaaggaggg
     4021 ccagagatgg ctgacagagg ccaggaagga gacagtgatg ggccagccaa cacccaagac
     4081 cccaagacag ctgagggagt tcctgggcaa agcaggattt tgcaggctgt tcatcccagg
     4141 attcgcagag atggcagcac ctctgtaccc actgaccaag ccgggcaccc tgtttaattg
     4201 gggccctgac cagcagaagg cctatcagga gatcaagcag gccctgctga cagcaccagc
     4261 cctgggcctg ccagacctga ccaagccttt cgagctgttt gtggatgaga agcagggcta
     4321 cgccaagggc gtgctgaccc agaagctggg accatggaga cggcccgtgg cctatctgtc
     4381 caagaagctg gacccagtgg cagcaggatg gccaccatgc ctgaggatgg tggcagcaat
     4441 cgccgtgctg acaaaggatg ccggcaagct gaccatggga cagccactgg tcatcctggc
     4501 accacacgca gtggaggccc tggtgaagca gcctccagat cgctggctgt ctaacgcccg
     4561 gatgacacac taccaggccc tgctgctgga caccgatcgc gtgcagtttg gccctgtggt
     4621 ggccctgaat ccatctggcg gctcaaaaag aaccgccgac ggcagcgaat tcgagtctcc
     4681 caagaagaag aggaaagtcg gctctggccc tgccgctaag agagtgaagc tggactaacc
     4741 ggtcatcatc accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct
     4801 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     4861 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     4921 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     4981 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     5041 ggctcgatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     5101 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     5161 aaagcctagg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     5221 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     5281 agaggcggtt tgcgtattgg gcgctacaga catgataaga tacattgatg agtttggaca
     5341 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     5401 tttatttgta accattataa gctgcaataa acaagttggg gtgggcgaag aactccagca
     5461 tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca
     5521 acctttcata gaaggcggcg gtggaatcga aatctcgtag cacgtgtcag tcctgctcct
     5581 cggccacgaa gtgcttagcc ctcccacaca taaccagagg gcagcaattc acgaatccca
     5641 actgccgtcg gctgtccatc actgtccttc actatggctt tgatcccagg atgcagatcg
     5701 agaagcacct gtcggcaccg tccgcagggg ctcaagatgc ccctgttctc atttccgatc
     5761 gcgacgatac aagtcaggtt gccagctgcc gcagcagcag cagtgcccag caccacgagt
     5821 tctgcacaag gtcccccagt aaaatgatat acattgacac cagtgaagat gcggccgtcg
     5881 ctagagagag ctgcgctggc gacgctgtag tcttcagaga tggggatgct gttgattgta
     5941 gccgttgctc tttcaatgag ggtggattct tcttgagaca aaggcttggc catggtttag
     6001 ttcctcacct tgtcgtatta tactatgccg atatactatg ccgatgatta attgtcaaca
     6061 cgtgctgatc agatccgaaa atggatatac aagctcccgg gagctttttg caaaagccta
     6121 ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggcagag gcggcctcgg
     6181 cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg
     6241 gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat
     6301 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg
     6361 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
     6421 accctaactg acacacattc cacagaattc actgactcgc tgcgctcggt cgttcggctg
     6481 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6541 aacgcaggaa agaacatgta agtgatctcc aaaaaataag tactttttga ctgtaaataa
     6601 aattgtaagg agtaaaaagt actttttttt ctaaaaaaat gtaattaagt aaaagtaaaa
     6661 gtattgattt ttaattgtac tcaagtaaag taaaaatccc caaaaataat acttaagtac
     6721 agtaatcaag taaaattact caagtacttt acacctctgc atgtgagcaa aaggccagca
     6781 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     6841 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     6901 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     6961 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7021 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7081 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7141 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7201 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7261 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7321 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7381 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7441 cactcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     7501 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     7561 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     7621 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     7681 gggcttacca tctggcccca gtgctgcaat gataccgcgg gacccacgct caccggctcc
     7741 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     7801 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     7861 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     7921 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     7981 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8041 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8101 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8161 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     8221 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     8281 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     8341 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     8401 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     8461 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     8521 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     8581 atcgggagat cgatctcccg atcccctagg gtcgactctc agtacaatct gctctgatgc
     8641 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     8701 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     8761 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gggtaccggc
     8821 atatggttct tgacagaggt gtaaaaagta ctcaaaaatt ttactcaagt gaaagtacaa
     8881 gtacttaggg aaaattttac tcaattaaaa gtaaaagtat ctggctagaa tcttacttga
     8941 gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt attaacgcgt tgacattgat
     9001 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     9061 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     9121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     9181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     9241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     9301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     9361 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     9421 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     9481 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     9541 ggcgtgtacg gtgggaggtc tatataagc
//
