LOCUS       sequence-412698-        3878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_MERS-CoV-nsp15
FEATURES             Location/Qualifiers
     source          1..3878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1189..1288)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1305..1324)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1306..1324)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1329..1345)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1329..1345)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1776..2567
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2660..3248
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3149..3168
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3402..3419
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3578..3605)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3697..3783)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3832..3854
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3846..3863
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3847..3863
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcagaag ggccgtcaag gccaggatcc
      121 atgggatccg gtttggaaaa cattgctttt aatgttgtta aacaaggcca ttttattggt
      181 gttgagggtg aactacctgt agctgtagtc aatgataaga tcttcaccaa gagtggcgtt
      241 aatgacattt gtatgtttga gaataaaacc actttgccta ctaatatagc ttttgaactc
      301 tatgctaagc gtgctgtacg ctcgcatccc gatttcaaat tgctacacaa tttacaagca
      361 gacatttgct acaagttcgt cctttgggat tatgaacgta gcaatattta tggtactgct
      421 actattggtg tatgtaagta cactgatatt gatgttaatt cagctttgaa tatatgtttt
      481 gacatacgcg ataattgttc attggagaag ttcatgtcta ctcccaatgc catctttatt
      541 tctgatagaa aaatcaagaa atacccttgt atggtaggtc ctgattatgc ttacttcaat
      601 ggtgctatca tccgtgatag tgatgttgtt aaacaaccag tgaagttcta cttgtataag
      661 aaagtcaata atgagtttat tgatcctact gagtgtattt acactcagag tcgctcttgt
      721 agtgacttcc tacccctttc tgacatggag aaagactttc tatcttttga tagtgatgtt
      781 ttcattaaga agtatggctt ggaaaactat gcttttgagc acgtagtcta tggagacttc
      841 tctcatacta cgttaggcgg tcttcacttg cttattggtt tatacaagaa gcaacaggaa
      901 ggtcatatta ttatggaaga aatgctaaaa ggtagctcaa ctattcataa ctattttatt
      961 actgagacta acacagcggc ttttaaggcg gtgtgttctg ttatagattt aaagcttgac
     1021 gactttgtta tgattttaaa gagtcaagac cttggcgtag tatccaaggt tgtcaaggtt
     1081 cctattgact taacaatgat tgagtttatg ttatggtgta aggatggaca ggttcaaacc
     1141 ttctaccctc gactccagct taattaagac gcgatcgcgg cctcatgggc ccagctttct
     1201 tgtacaaagt tggcattata aaaaataatt gctcatcaat ttgttgcaac gaacaggtca
     1261 ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt
     1321 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     1381 acattgcaca agataaaaat atatcatcat gcctcctcta gaccagccag gacagaaatg
     1441 cctcgacttc gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg
     1501 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     1561 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     1621 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     1681 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     1741 agcagggcag tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag
     1801 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     1861 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     1921 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     1981 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     2041 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     2101 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     2161 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     2221 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     2281 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     2341 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     2401 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     2461 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     2521 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc
     2581 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     2641 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2701 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     2761 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     2821 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     2881 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     2941 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3001 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3061 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3121 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3181 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3241 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3301 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     3361 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3421 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     3481 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
     3541 cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
     3601 cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
     3661 ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
     3721 caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat
     3781 ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac
     3841 gacgttgtaa aacgacggcc agtcttaagc tcgggccc
//
