LOCUS       sequence-412699-        3825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pDONR223 MERS-CoV NSP15_stop
FEATURES             Location/Qualifiers
     source          1..3825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1136..1235)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1252..1271)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1253..1271)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1276..1292)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1276..1292)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1723..2514
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2607..3195
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3096..3115
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3349..3366
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3525..3552)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3644..3730)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3779..3801
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3793..3810
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3794..3810
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tgggtttgga aaacattgct
      121 tttaatgttg ttaaacaagg ccattttatt ggtgttgagg gtgaactacc tgtagctgta
      181 gtcaatgata agatcttcac caagagtggc gttaatgaca tttgtatgtt tgagaataaa
      241 accactttgc ctactaatat agcttttgaa ctctatgcta agcgtgctgt acgctcgcat
      301 cccgatttca aattgctaca caatttacaa gcagacattt gctacaagtt cgtcctttgg
      361 gattatgaac gtagcaatat ttatggtact gctactattg gtgtatgtaa gtacactgat
      421 attgatgtta attcagcttt gaatatatgt tttgacatac gcgataattg ttcattggag
      481 aagttcatgt ctactcccaa tgccatcttt atttctgata gaaaaatcaa gaaataccct
      541 tgtatggtag gtcctgatta tgcttacttc aatggtgcta tcatccgtga tagtgatgtt
      601 gttaaacaac cagtgaagtt ctacttgtat aagaaagtca ataatgagtt tattgatcct
      661 actgagtgta tttacactca gagtcgctct tgtagtgact tcctacccct ttctgacatg
      721 gagaaagact ttctatcttt tgatagtgat gttttcatta agaagtatgg cttggaaaac
      781 tatgcttttg agcacgtagt ctatggagac ttctctcata ctacgttagg cggtcttcac
      841 ttgcttattg gtttatacaa gaagcaacag gaaggtcata ttattatgga agaaatgcta
      901 aaaggtagct caactattca taactatttt attactgaga ctaacacagc ggcttttaag
      961 gcggtgtgtt ctgttataga tttaaagctt gacgactttg ttatgatttt aaagagtcaa
     1021 gaccttggcg tagtatccaa ggttgtcaag gttcctattg acttaacaat gattgagttt
     1081 atgttatggt gtaaggatgg acaggttcaa accttctacc ctcgactcca gtaataccca
     1141 actttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     1201 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     1261 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     1321 tgatgttaca ttgcacaaga taaaaatata tcatcatgcc tcctctagac cagccaggac
     1381 agaaatgcct cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg
     1441 atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
     1501 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     1561 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
     1621 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     1681 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc
     1741 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     1801 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
     1861 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     1921 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     1981 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     2041 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     2101 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     2161 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     2221 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     2281 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     2341 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     2401 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     2461 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg
     2521 agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
     2581 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2641 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2701 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2761 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2821 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2881 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2941 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     3001 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3061 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3121 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3181 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3241 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3301 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     3361 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     3421 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     3481 attaatacgc gtaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca
     3541 ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc
     3601 ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga
     3661 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc
     3721 gttttatttg atgcctggca gttccctact ctcgcgttaa cgctagcatg gatgttttcc
     3781 cagtcacgac gttgtaaaac gacggccagt cttaagctcg ggccc
//
