LOCUS       sequence-41272-e         646 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Lien AD, Scanziani M
  TITLE     In vivo Labeling of Constellations of Functionally Identified 
            Neurons for Targeted in vitro Recordings.
  JOURNAL   Front Neural Circuits. 2011;5:16. Epub 2011 Nov 29.
  PUBMED    22144948
REFERENCE   2  (bases 1 to 646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Neural Circuits."; date: "2011"; volume: "5"; pages: "16. Epub 2011 
            Nov 29"
COMMENT     Sequence Label: pACAGW-H2B-PAGFP-AAV
FEATURES             Location/Qualifiers
     source          1..646
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(41..418)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     primer_bind     complement(529..548)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(610..632)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggtggatc cttagcgctg gtgtacttgg
       61 tgatggcctt agtaccctcg gacacggcgt gcttggccaa ctccccaggc agcagcaggc
      121 gcacggccgt ctggatctcc ctggaggtga tggtcgagcg cttgttgtaa tgcgccaggc
      181 gggaagcctc acctgcgatg cgctcgaaaa tgtcgttcac aaacgaattc atgatgccca
      241 tggccttgga cgaaatgccg gtgtcagggt ggacctgctt cagaaccttg tacacataga
      301 tggaatagct ctccttgcgg ctgcgcttgc gcttcttgcc gcctttcttc tgcgccttag
      361 tcaccgcctt cttggagccc tttttcgggg cgggagcaga cttcgctggc tctggcatgg
      421 tggcggtacc gtcgactgca gaattcgaag cttgagctcg agatctgagt ccggtagcgc
      481 tagcaccggt ggatcccgat ccaattcttt gccaaaatga tgagacagca caataaccag
      541 cacgttgccc aggagctgta ggaaaaagaa gaaggcatga acatggttag cagaggctct
      601 agagccgccg gtcacacgcc agaagccgaa ccccgccctg ccccgt
//
