LOCUS       sequence-412768-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression Plasmid of Mitofusin-1 scFv. Derived from 
            hybridoma N111/24..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Mitchell KG, Gong B, Hunter SS, Burkart-Waco D, Gavira-O'Neill CE, 
            Templeton KM, Goethel ME, Bzymek M, MacNiven LM, Murray KD, Settles 
            ML, Froenicke L, Trimmer JS
  TITLE     High-volume hybridoma sequencing on the NeuroMabSeq platform enables
            efficient generation of recombinant monoclonal antibodies and scFvs 
            for neuroscience research.
  JOURNAL   Sci Rep. 2023 Sep 27;13(1):16200. doi: 10.1038/s41598-023-43233-4.
  PUBMED    37758930
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-09-27"; volume: "13(1):16200. doi"; pages: " 
            10.1038/s41598-023-43233-4"
COMMENT     Sequence Label: Mitofusin-1 scFv [N111/24]
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             781..807
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             832..849
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggctgga gctgtataat tttgttcttg gtggcgaccg caacaggagt acattcagag
       61 gttaagctcg aagaaagtgg cggcgggctg gtccagccag gccgcagcat gaagttgtcc
      121 tgcgcagcgt ccggctttag cttctccgat gcttggatgg attgggtgag gcaatccccc
      181 gaaaagggtc tggaatgggt ggccgagatc aggtctaagg cgaacaacca cgctacttat
      241 tacgcggaat ccgtaaaggg cagatttact atatcccgtg acgacagcaa gtcttccgtg
      301 tatttacaga tgaattctct tagggcagag gatacaggaa tatactattg caccgtggtg
      361 gctggaaatt attggggtca agggacgaca ttgactgtga gttccggagg aggtggatcc
      421 ggtggcggcg ggtctggtgg tggtgggtca ggtggtggaa gccagatcat actgactcaa
      481 agtcctgcga ttatgagcgc tagtcctggc gaaaaggtga ctattacatg ttctgcatct
      541 agcagcgtgt cttttatgta ttggtttcaa cagaaacccg ggaccagccc gaagttttgg
      601 atctacagca cctctaatct tgccagcggg gtgccggcca gattttccgg aagcgggagc
      661 ggcactagct atagcctgac catatcacgc atggaagctg aggacgcagc tacctactat
      721 tgtcaacaga gaaactcata tccgtttacc tttgggtctg gtaccaaatt agaggttaag
      781 tatccctatg atgtccccga ctatgcatcc ggcctgcccg aaacaggcgg ccatcatcat
      841 catcatcact ag
//
