LOCUS       sequence-412769-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression Plasmid of Botch scFv. Derived from hybridoma 
            N116/14..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Mitchell KG, Gong B, Hunter SS, Burkart-Waco D, Gavira-O'Neill CE, 
            Templeton KM, Goethel ME, Bzymek M, MacNiven LM, Murray KD, Settles 
            ML, Froenicke L, Trimmer JS
  TITLE     High-volume hybridoma sequencing on the NeuroMabSeq platform enables
            efficient generation of recombinant monoclonal antibodies and scFvs 
            for neuroscience research.
  JOURNAL   Sci Rep. 2023 Sep 27;13(1):16200. doi: 10.1038/s41598-023-43233-4.
  PUBMED    37758930
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-09-27"; volume: "13(1):16200. doi"; pages: " 
            10.1038/s41598-023-43233-4"
COMMENT     Sequence Label: Botch scFv [N116/14]
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             805..831
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             856..873
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggctgga gctgtattat tctcttcttg gtggcgaccg caactggagt acattcccaa
       61 gtgcaactcc aacaaagcgg cgccgaactc atgaaacccg gagcgtctgt caaaattagt
      121 tgtaaagcca cgggttatac ttttaacaat tattggattg aatggatcaa acaacgtccc
      181 ggccacggac tcgaatggat cggcgaaatc ttgccggggt cagactctaa ttataacgaa
      241 aagtttaaag gaaaagcaac gtttacgact gacacgagca gcaatactgc atatatccag
      301 ctgtcatccc tcacttcaga agatagtgct gtatactatt gctcccgggg ttacggctat
      361 tttacatggt ttgcctattg gggacaggga acactcgtaa cagtgagtgc tggtggtggt
      421 ggatcaggtg gcggcggttc aggtggtggt gggtcaggcg gtggatccga cgtggtcatg
      481 acacagaccc ctttaagcct gccagtttcc ctgggcgacc aggcaagcat tagttgtcgt
      541 agctcccaaa gtctggtgca tagcaacggt aatacatact tgcactggta tctccagaaa
      601 cctggtcaat cacctaaact gctcatttat aaggtaagca atcgattcag cggagttccc
      661 gatcgctttt ccggttcagg ttccggcacg gactttactc ttaaaatttc tcgtgtagaa
      721 gcagaagacc ttgggatcta cttttgttcc caggggaccc acgtcccata tacctttggt
      781 ggcggaacaa aacttgagat taagtatccc tatgatgtac ctgactatgc ttccggcctg
      841 cccgaaacag gcggtcatca tcatcatcat cactaa
//
