LOCUS       sequence-412770-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression Plasmid of Pan-QKI scFv. Derived from hybridoma
            N147/6..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Mitchell KG, Gong B, Hunter SS, Burkart-Waco D, Gavira-O'Neill CE, 
            Templeton KM, Goethel ME, Bzymek M, MacNiven LM, Murray KD, Settles 
            ML, Froenicke L, Trimmer JS
  TITLE     High-volume hybridoma sequencing on the NeuroMabSeq platform enables
            efficient generation of recombinant monoclonal antibodies and scFvs 
            for neuroscience research.
  JOURNAL   Sci Rep. 2023 Sep 27;13(1):16200. doi: 10.1038/s41598-023-43233-4.
  PUBMED    37758930
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-09-27"; volume: "13(1):16200. doi"; pages: " 
            10.1038/s41598-023-43233-4"
COMMENT     Sequence Label: Pan-QKI scFv [N147/6]
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             805..831
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             856..873
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggctgga gctgtataat tctctttctc gtagcgaccg cgactggagt ccattccgac
       61 gtacaattgc aagaatctgg cccggggttg gtccgcccat cccaatcact cagcttaaca
      121 tgtaccgtga ccggttatag tataacgtct gactacgcat ggtattggat tagacaattc
      181 ccagggaata agctcgaatg gatgggttat atcagtttct cagacagtac gtcttataat
      241 ccgagcttaa agagcagagt gtcaattacc cgtgatacca gtaagaatca attctttctt
      301 caactcaaca gtgtaaccac agaagatacc gcgacttact attgctcccg gagaagatat
      361 tatgggagct catccggcta ttttgacgta tggggagctg gaactaccgt gactgttagt
      421 tccggtggag gaggatccgg cggaggaggt tccggcggtg gtggaagtgg cggcggttca
      481 cagatcgtgc tgactcaaag tccggcgata atgtcagcta gtctgggcga gcgcgttaca
      541 atgacttgta cagcatccag ctctgtgtcc agcacctacc tgcattggta tcaacaaaag
      601 cctggctcta gcccgaagct ttggatctat tccaccagca atctcgccag cggcgtgccc
      661 gtgcggtttt ccggatccgg aagcggaact tcattttcct taacgattag ttctatggaa
      721 gcagaggacg ccgctacata ctattgtcat caataccacc gctctccccc tacttttggc
      781 ggcggaacaa aactcgagat aaagtatccg tatgatgtac ctgactatgc tagcggcctg
      841 ccagaaacag gcgggcatca tcatcatcat cactag
//
