LOCUS       sequence-412772-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression Plasmid of TRIP8b (exon 4) scFv. Derived from 
            hybridoma N212/3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Mitchell KG, Gong B, Hunter SS, Burkart-Waco D, Gavira-O'Neill CE, 
            Templeton KM, Goethel ME, Bzymek M, MacNiven LM, Murray KD, Settles 
            ML, Froenicke L, Trimmer JS
  TITLE     High-volume hybridoma sequencing on the NeuroMabSeq platform enables
            efficient generation of recombinant monoclonal antibodies and scFvs 
            for neuroscience research.
  JOURNAL   Sci Rep. 2023 Sep 27;13(1):16200. doi: 10.1038/s41598-023-43233-4.
  PUBMED    37758930
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-09-27"; volume: "13(1):16200. doi"; pages: " 
            10.1038/s41598-023-43233-4"
COMMENT     Sequence Label: TRIP8b (exon 4) scFv [N212/3]
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             802..828
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             853..870
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggctgga gctgtattat actctttctt gtagctaccg cgacaggagt tcattcccaa
       61 gtacaattac aacaatccgg tgccgaattg gtcaaaccag gcgcaagcgt taaaataagc
      121 tgtcgcgcct caggttatac gtttagcact tattggattc aatgggtcaa acaacggcca
      181 ggccacgggt tagaatggat cggggaaatt ctcccaggca acatctcaac aaattataac
      241 gaaaagttta aaggaaaagc aacttttacg gccgacactt catcaaatac cgcttatatg
      301 cagctgtcca gtctcccgag cgaagattca gctgtgtact attgcgctcg tctgcggttg
      361 gggtacgcaa tggattattg gggccagggt acgagcgtga cggtgagttc cggcggagga
      421 gggtcaggtg gtggtggatc aggtgggggg gggtcaggtg gcgggagcga tattgtactc
      481 acacagagtc ccgcatccct tgctgtcagt ctcggccaac gcgcaactat tagttgtagg
      541 gcttctgagt ccgtggactc ttacggtaac aacttcatgc attggtatca acaaaagccc
      601 ggccaaccgc ctaagctgct tatttaccgg gcctcaaatc tggagagtgg cattccggct
      661 agattttccg ggagcggaag ccgcactgat tttacgttga ctataaaccc ggttgaagcc
      721 gacgacgtgg cgacttacta ttgccaacag aacaacgaag acccttggac ctttggcggc
      781 ggaacaaaat tggagattaa gtatccatat gatgtccctg actatgcttc aggcctgcca
      841 gaaactggcg gtcatcatca tcatcatcac t
//
