LOCUS       sequence-412773-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression Plasmid of Synaptotagmin-7 scFv. Derived from 
            hybridoma N275/14..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Mitchell KG, Gong B, Hunter SS, Burkart-Waco D, Gavira-O'Neill CE, 
            Templeton KM, Goethel ME, Bzymek M, MacNiven LM, Murray KD, Settles 
            ML, Froenicke L, Trimmer JS
  TITLE     High-volume hybridoma sequencing on the NeuroMabSeq platform enables
            efficient generation of recombinant monoclonal antibodies and scFvs 
            for neuroscience research.
  JOURNAL   Sci Rep. 2023 Sep 27;13(1):16200. doi: 10.1038/s41598-023-43233-4.
  PUBMED    37758930
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-09-27"; volume: "13(1):16200. doi"; pages: " 
            10.1038/s41598-023-43233-4"
COMMENT     Sequence Label: Synaptotagmin-7 scFv [N275/14]
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             793..819
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             844..861
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggctgga gctgtattat tcttttcctt gtggctaccg caacaggagt tcattccgaa
       61 gtccaattac aagaaagtgg ccccaatctg gtcaagccca cccaaaccct ttctctgaca
      121 tgctccgtga ccggtgatag cattacatct gactactgga attggataag aaagtttccg
      181 ggtaacaagt tcgaatatat gggttatatc tcatatggcg gcataaccta ttataacccc
      241 agtctgaaga gcagaatttc aattacgcgg gataccagta agaatcacta ctaccttcac
      301 ctgaactcag taaccaccga agatacggcg acgtactatt gcgtccgcga tgggtattca
      361 ggcacaaatt atgaagccta ttggggacag ggcacattgg tgaccgtttc cgctggtggt
      421 ggcggatcag gcggtggcgg atccggtggt ggcggatctg gtggcgggag tgacatacaa
      481 atgacccaaa cgactagctc tttgtccgca agcctcgggg ataagattac tattagctgt
      541 tccgcgtctc aagatatctc acactacctg agttggtacc aacaaaagcc cgacggtaca
      601 gtcaagctgt taattcacta tacgtctaag ctgcattctg gcgttcctag ccgctttagc
      661 gggtcaggca gtggtaccga ctacagcctg actatttctt atttagagcc agaggacata
      721 gcaacgtatt actgccaaca gtactctaat ttgccatata catttggcgg cggtacgaaa
      781 ctcgagatta agtatcccta tgatgttcct gactatgcta gtggactgcc agaaactggt
      841 ggccatcatc atcatcatca ctag
//
