LOCUS       sequence-412774-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian Expression Plasmid of Ankyrin-R scFv. Derived from 
            hybridoma N388A/10..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Mitchell KG, Gong B, Hunter SS, Burkart-Waco D, Gavira-O'Neill CE, 
            Templeton KM, Goethel ME, Bzymek M, MacNiven LM, Murray KD, Settles 
            ML, Froenicke L, Trimmer JS
  TITLE     High-volume hybridoma sequencing on the NeuroMabSeq platform enables
            efficient generation of recombinant monoclonal antibodies and scFvs 
            for neuroscience research.
  JOURNAL   Sci Rep. 2023 Sep 27;13(1):16200. doi: 10.1038/s41598-023-43233-4.
  PUBMED    37758930
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2023-09-27"; volume: "13(1):16200. doi"; pages: " 
            10.1038/s41598-023-43233-4"
COMMENT     Sequence Label: Ankyrin-R scFv [N388A/10]
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             781..807
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             832..849
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggctgga gctgtattat tcttttcttg gtggctaccg ctacaggggt tcattccgag
       61 gttaaactcg tagaatccgg cggtggtttg gtaagaccag gtggaagcct taagctttca
      121 tgcgccgcaa gcgggtttac attttcttct tacgctatga gctgggtccg tcaaaccccg
      181 gagaaaagat tggaatgggt ggccagcatc tcttcaggcg gacggactta ttaccctgat
      241 tctgttaaag gtcggtttac gattagccga gacgacgcga gaaatattct ctatctccag
      301 atgtctagct tgagatcaga agataccgct atgtactatt gcgcccgaga ttcttacgag
      361 ttcgcatatt ggggacaggg aaccctcgtt acggtttccg ccggtggcgg tggttctggt
      421 ggtggtggat cagggggggg tggaagtggc ggcggtagtg atatacaaat gacccagtcc
      481 tcaagctatc tgagcgtgag cctgggcggg cgcgttacta tcacatgtaa agccagcgat
      541 catatcaaca actggttggc atggtaccaa cagaagcccg ggaacgcacc aagactgctc
      601 atcagtggcg ccacaaacct ggagacggga gtgccaagcc ggttttccgg aagcggtagt
      661 ggtaaagact ataccctgtc catcacttct ttacaaacag aggacgtagc aacatactat
      721 tgccagcaat actggtcaac cccatataca tttggcggcg gaactaaatt ggagattaag
      781 tatccctatg atgttcctga ctatgcgagt ggtctgccgg aaacaggtgg ccatcatcat
      841 catcatcact ag
//
