LOCUS       sequence-412878-       13777 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tetracycline inducible expression vector for HA-JSAP1_WT, used as a 
            donor plasmid for HA-JSAP1_WT knock-in at the human ROSA26 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13777)
  AUTHORS   Suzuki R, Kanemaki MT, Suzuki T, Yoshioka K
  TITLE     Overexpression of JNK-associated leucine zipper protein induces 
            chromosomal instability through interaction with dynein light 
            intermediate chain 1.
  JOURNAL   Genes Cells. 2023 Nov 14. doi: 10.1111/gtc.13083.
  PUBMED    37963657
REFERENCE   2  (bases 1 to 13777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Cells. 2023 Nov 14. doi: 10.1111/gtc.13083."
COMMENT     Sequence Label: phROSA26-Tet-HA-JSAP1_WT
FEATURES             Location/Qualifiers
     source          1..13777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2323..2404
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2521..3267)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(3286..3796)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3349..3367)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     polyA_signal    3816..3864
                     /note="synthetic polyadenylation signal"
     misc_feature    3878..3969
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3918..3937
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        3973..4340
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    3981..3999
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4017..4035
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4053..4071
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4089..4107
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4125..4143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4161..4179
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4197..4215
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             4355..4381
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    8511..8592
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    8642..8675
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        8772..9129
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8772..8963
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8795..8815)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8980..9115
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9164..9958
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9164..9958
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9218..9237)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9828..9847
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    10464..10497
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(11589..11609)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11589..11607)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11628..11644)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11628..11644)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11641..11663)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11652..11668
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11676..11682,11683..11700,11701..11706))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11721..11742
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(12030..12618)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12110..12129)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12789..13580,13581..13649))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13412..13431
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13650..13754)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcag
      661 tggcagcatc ctcctaagag cttggggagg gccaggccca cgacccaagg agagcgagcg
      721 cggggagacg gaggaggtga cccttccctc ccctggggcc cgatcgtgag gttcggtctc
      781 ttttctgtcg gacccttacc ttgtcccagg cgctgccggg gcctgggccc gggctgcggc
      841 gcacggcact cccgggaggc ggcaggactc gagttaggcc caacgcggcg ccacggcgtt
      901 tcctggccgg gaatggcccg tacccgtgag gtgggggtgg ggggcagaaa ggcggagcga
      961 gccaaaggcg gggagggggg gcagggccag ggaaagaggg gggccggcac tactgtgttg
     1021 gcggactggc gggactgggg ctgcgtgagt ctctgagcgc aggcgggcgg cggccgcccc
     1081 tcccccggca gcggcggcgg cggcggcggc ggcggcggcg gcagctcact cagcccgctg
     1141 cccgagcgga aacgccactg accgcacggg gattcccagc gccggcgcca ggggcacccg
     1201 ggacacgccc cctcccgccg cgccattggc ctctccgccc accgccccgc acccattggc
     1261 ccactcgccg ccaatcagcg gaagccgccg gggccgccta gagaagaggc tgtgcttcgg
     1321 cgctccggct cctcagagag cctcggctag gtaggggagc ggaactctgg tgggagggga
     1381 ggtgcggtgc actgggggga tgggtggcta ggggggccgt ctggtggctt gcgggggttg
     1441 cctttcccgt gggaagtcgg gaacataatg tttgttacgt tgggagggaa aggggtggct
     1501 ggatgcaggc gggagggagg cccgccctgc ggcaaccgga gggggaggga gaagggagcg
     1561 gaaaatgctc gaaaccggac ggagccattg ctctcgcaga gggaggagcg cttccggcta
     1621 gcctcttgtc gccgattggc cgtttctcct cccgccgtgt gtgaaaacac aaatggcgta
     1681 ttctggttgg agtaaagctc ctgtcagtta cgccgtcggg agtacgcagc cgcttagcga
     1741 ctctcgcgtt gccccctggg tggggcgggt aggtaggtgg ggtgtagaga tgctgggtgt
     1801 gcgggcgcgg ccggcctcct gcggcgggag gggagggtca gtgaaattgg ctctggcgcg
     1861 ggcgtcctcc caccctcccc ttccttcggg ggagtcggtt tacccgccgc ctgcttgtct
     1921 tcgacacctg attggctgtc gaagctgtgg gaccgggccc ttgctactgg ctcgagtctc
     1981 acatgagcga aaccactgcg cggggcgcgg gggtggcggg gaggcgggcg ttggtacggt
     2041 cctccccgag gccgagcgcc gcagtgtctg gccccgcgcc cctgcgcaac gtggcaggaa
     2101 gcgcgcgctg gaggcggggg cgggctgccg gccgagactt ctggatggcg gcggccgcgg
     2161 ctccgccccg ggttcccacc gcctgaaggg cgagacaagc ccgacctgct acaggcactc
     2221 gtgggggtgg gggaggagcg ggggtcggtc cggctggttt gtgggtggga ggcgcttgtt
     2281 ctccaaaaac cggcgcggag tcagtgagcg aggaagctcg gggcagtgaa aaaaatgctt
     2341 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     2401 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     2461 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc ctctggagat
     2521 ttacccgggg agcatgtcaa ggtcaaaatc gtcaagagcg tcagcaggca gcatatcaag
     2581 gtcaaagtcg tcaagggcat cggctgggag catgtctaag tcaaaatcgt caagggcgtc
     2641 ggtcggcccg ccgctttcgc actttagctg tttctccagg ccacatatga ttagttccag
     2701 gccgaaaagg aaggcaggtt cggctccctg ccggtcgaac agctcaattg cttgtttcag
     2761 aagtgggggc atagaatcgg tggtaggtgt ctctctttcc tcttttgcta cttgatgctc
     2821 ctgttcctcc aatacgcagc ccagtgtaaa gtggcccacg gcggacagag cgtacagtgc
     2881 gttctccagg gagaagcctt gctgacacag gaacgcgagc tgattttcca gggtttcgta
     2941 ctgtttctct gttgggcggg tgccgagatg cactttagcc ccgtcgcgat gtgagaggag
     3001 agcacagcgg tatgacttgg cgttgttccg cagaaagtct tgccatgact cgccttccag
     3061 ggggcaggag tgggtatgat gcctgtccag catctcgatt ggcagggcat cgagcagggc
     3121 ccgcttgttc ttcacgtgcc agtacagggt aggctgctca actcccagct tttgagcgag
     3181 tttccttgtc gtcaggcctt cgataccgac tccattgagt aattccagag cagagtttat
     3241 gactttgctc ttgtccagtc tagacatggt aattcgatga tccccctggg gagagaggtc
     3301 ggtgattcgg tcaacgaggg agccgactgc cgacgtgcgc tccggaggct tgcagaatgc
     3361 ggaacaccgc gcgggcagga acagggccca cactaccgcc ccacaccccg cctcccgcac
     3421 cgccccttcc cggccgctgc tctcggcgcg ccctgctgag cagccgctat tggccacagc
     3481 ccatcgcggt cggcgcgctg ccattgctcc ctggcgctgt ccgtctgcga gggtactagt
     3541 gagacgtgcg gcttccgttt gtcacgtccg gcacgccgcg aaccgcaagg aaccttcccg
     3601 acttaggggc ggagcaggaa gcgtcgccgg ggggcccaca agggtagcgg cgaagatccg
     3661 ggtgacgctg cgaacggacg tgaagaatgt gcgagaccca gggtcggcgc cgctgcgttt
     3721 cccggaacca cgcccagagc agccgcgtcc ctgcgcaaac ccagggctgc cttggaaaag
     3781 gcgcaacccc aaccccgtgg aattatcacc tcgagaataa aatatcttta ttttcattac
     3841 atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca
     3901 aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa
     3961 catttctctc tcgagtttac tccctatcag tgatagagaa cgtatgaaga gtttactccc
     4021 tatcagtgat agagaacgta tgcagacttt actccctatc agtgatagag aacgtataag
     4081 gagtttactc cctatcagtg atagagaacg tatgaccagt ttactcccta tcagtgatag
     4141 agaacgtatc tacagtttac tccctatcag tgatagagaa cgtatatcca gtttactccc
     4201 tatcagtgat agagaacgta taagctttgc ttatgtaaac cagggcgcct ataaaagagt
     4261 gctgattttt tgagtaaact tcaattccac aacacttttg tcttatacca actttccgta
     4321 ccacttccta ccctcgtaaa gaattgccac catgtacccc tatgatgtgc cagactacgc
     4381 cgatatcgag atccagatgg acgagggcgg cggcgtggtg gtgtaccagg acgactactg
     4441 ctccggctcg gtgatgtcgg agcgggtgtc gggcctggcg ggctccatct accgcgagtt
     4501 cgagcgcctc atccactgct acgacgagga ggtggtcaag gagctcatgc cgctggtggt
     4561 gaacgtgctg gagaacctag actcggtgct cagcgagaac caggagcacg aggtggagct
     4621 ggagctgctg cgcgaggaca acgagcagct gctcacccag tacgagcgtg agaaggcgct
     4681 gcgcaggcag gcggaggaga aattcattga gtttgaagat gctctggaac aagagaagaa
     4741 agagctgcaa atccaggtgg agcactacga gttccagacg cgccagctgg agctgaaggc
     4801 caagaactat gccgatcaga tttcccggtt ggaggagcgg gagtcggaga tgaagaagga
     4861 gtacaatgcc ctgcaccagc ggcacacaga gatgatacag acctacgtgg agcacattga
     4921 gaggtccaag atgcagcagg tcggaggaaa cagccagacc gagagcagcc tgccggggcg
     4981 gaggaaggag cgccccacct ccctgaacgt gttccccctg gctgacggca cggtacgtgc
     5041 acagatcggg ggcaagctcg tgcctgcggg ggaccactgg cacctgagtg acctcggcca
     5101 gctgcagtcc agctccagct accagtgtcc acaggatgaa atgtccgagt caggccagtc
     5161 ctcggcggcc gccacaccca gcaccacagg caccaagtcc aacacgccca catcctccgt
     5221 gccctcggcc gccgtcacac ccctcaacga gagcctgcag cccctggggg actatggcgt
     5281 gggctccaag aacagcaagc gtgcccggga gaagcgcgac agccgcaaca tggaagtaca
     5341 ggtcacccag gagatgcgca acgtcagtat aggcatgggc agcagtgacg agtggtctga
     5401 tgttcaagac attattgact ccacgccaga gctggacatg tgtccagaga cccgcctgga
     5461 ccgcacagga agcagcccaa cccagggcat cgtgaacaaa gctttcggca tcaacaccga
     5521 ctccctgtac catgagctgt cgacggcagg gtctgaggtc atcggggatg tggacgaagg
     5581 ggccgacctc ctaggggagt tctcaggaat gggcaaagaa gtggggaatc tgctactgga
     5641 aaactcacag cttctggaaa ccaaaaacgc cttgaatgtg gtgaagaatg acctgattgc
     5701 caaggtcgac cagctgtccg gggagcagga ggtgctgagg ggcgagttgg aggctgctaa
     5761 gcaggccaaa gtcaagctgg aaaaccgtat caaggagctg gaagaggaac tgaaaagagt
     5821 gaagtccgag gccatcatcg cccgccgtga acccaaagaa gaggcggagg atgtaagcag
     5881 ctatctctgt acagaatcgg acaaaatccc catggcccag cgccgccgct tcacgcgggt
     5941 ggagatggcc cgtgtgctca tggagcggaa ccagtacaag gagcggctga tggagctgca
     6001 ggaggctgtg cggtggactg agatgatcag agcgtcccga gagcacccat ccgtccagga
     6061 gaagaagaag tcgaccatct ggcagttctt cagccgcctc ttcagctctt cctccagccc
     6121 ccctccggcc aagcgcccct atccctcggt gaacatccac tacaagtcac ccaccactgc
     6181 cggcttcagc cagcgccgca accatgccat gtgcccgatc tcggcaggca gccggcccct
     6241 ggaattcttc cctgacgacg actgcacgtc ctccgcccgt cgagagcaga agcgcgagca
     6301 gtaccgccag gtgcgtgagc acgtgcgtaa cgacgacggc cgtctgcagg cctgcggctg
     6361 gagcctgccc gccaagtaca agcagctgag tcccaacggg ggccaggagg acacgcggat
     6421 gaagaacgtg ccggtgccgg tgtactgccg ccctctggtg gagaaggacc ccaccatgaa
     6481 gctgtggtgt gccgcgggcg tcaacctgag cgggtggagg cccaatgagg acgacgctgg
     6541 gaatggagtc aagccagcgc caggccgcga tcccctgacc tgcgaccgcg aaggagacgg
     6601 cgagcccaag agcgcccaca cgtctcccga gaagaagaag gccaaggagc tccctgaaat
     6661 ggacgccacc tccagccggg tgtggatcct gaccagcacc ctgaccacca gcaaggtggt
     6721 gatcatcgac gccaaccagc cgggcacggt ggtggaccag ttcaccgtct gcaacgcgca
     6781 cgtgctgtgc atctccagca tccccgcggc cagcgacagc gactaccctc ccggggagat
     6841 gttcctggac agcgacgtga acccagagga cccgggcgca gatggcgtgc tggccggtat
     6901 caccctggtg ggctgtgcca cccgctgcaa cgtgccgcgg agcaactgct cctcccgagg
     6961 ggacacccca gtgctagaca aggggcaggg ggaggtggcc accatcgcca acgggaaggt
     7021 caacccgtcc cagtccacag aggaggccac agaggccacg gaggtgccag accctgggcc
     7081 cagcgagcca gagacagcca cattgcggcc cgggcctctc acagagcacg tcttcactga
     7141 cccagccccg accccgtcct ctggccccca gcctggcagc gagaacgggc cagagcctga
     7201 cagcagcagc acacggccag agccagagcc cagcggggac cccacgggag caggcagcag
     7261 tgctgcaccc accatgtggc tgggagccca gaacggctgg ctctatgtgc actcggctgt
     7321 ggccaactgg aagaagtgcc tgcactccat caagctgaag gattctgtgc tgagcctggt
     7381 gcatgtcaaa ggccgtgtgc tggtggctct ggcggacggg accctggcca tcttccaccg
     7441 tggtgaagat ggccagtggg atctgagcaa ctatcaccta atggacctgg gccacccgca
     7501 ccactccatc cgctgcatgg ctgttgtgta cgaccgcgtg tggtgtggct acaagaacaa
     7561 ggtgcacgtc atccagccca agaccatgca gatagagaag tcatttgacg cccacccgcg
     7621 gcgggagagc caggtgcggc agctggcgtg gatcggcgat ggcgtatggg tgtccatccg
     7681 cctggactcc accctgaggc tctaccatgc acacacgcac cagcatctac aggacgtgga
     7741 cattgagccc tacgtcagca agatgctagg cactggcaag ctgggtttct ccttcgtacg
     7801 catcacggcc ctgcttgtcg cgggcagccg gctctgggtg ggcaccggca acggagtggt
     7861 catctccatc cccctgacag agactgtggt cctgcaccga ggccagctcc tggggctccg
     7921 agccaataag acatccccca cctctgggga gggcgcccgt cccgggggca tcatccacgt
     7981 gtatggcgat gacagcagtg acagggcggc cagcagcttc atcccctact gctccatggc
     8041 ccaggcccag ctatgcttcc atgggcaccg cgatgccgtg aagttctttg tctcggtgcc
     8101 agggaacgtg ctggccaccc tgaatggcag tgtgctggac agcccagccg agggccctgg
     8161 gccagctgcc cctgcctcgg aggtcgaggg ccagaagctg cggaacgtgc tggtgctgag
     8221 cggcggggag ggctacatcg acttccgcat tggagacgga gaggacgacg agacggagga
     8281 gggcgcaggg gacatgagcc aggtgaagcc cgtgctgtcc aaggcagagc gcagtcacat
     8341 catcgtgtgg caggtgtcct acacccccga gtgagatcca gatctctgca gcatatgtct
     8401 agaggatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     8461 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     8521 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     8581 ttttttcact gccgacgtca ggtggcactt ttcggggcga tcatattcaa taacccttaa
     8641 tataacttcg tataatgtat gctatacgaa gttattaggt ctgaagagga gtttacgtcc
     8701 agccaagctt aggatctcga cctcgaaatt ctaccgggta ggggaggcgc ttttcccaag
     8761 gcagtctgga gctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     8821 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     8881 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     8941 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     9001 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     9061 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     9121 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     9181 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     9241 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     9301 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     9361 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     9421 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     9481 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     9541 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     9601 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     9661 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     9721 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     9781 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     9841 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     9901 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     9961 gtcgatgata tcagatcccc gggatgcaga aattgatgat ctattaaaca ataaagatgt
    10021 ccactaaaat ggaagttttt cctgtcatac tttgttaaga agggtgagaa cagagtacct
    10081 acattttgaa tggaaggatt ggagctacgg gggtgggggt ggggtgggat tagataaatg
    10141 cctgctcttt actgaaggct ctttactatt gctttatgat aatgtttcat agttggatat
    10201 cataatttaa acaagcaaaa ccaaattaag ggccagctca ttcctcccac tcatgatcta
    10261 tagatctata gatctctcgt gggatcattg tttttctctt gattcccact ttgtggttct
    10321 aagtactgtg gtttccaaat gtgtcagttt catagcctga agaacgagat cagcagcctc
    10381 tgttccacat acacttcatt ctcagtattg ttttgccaag ttctaattcc atcagaagct
    10441 ggtcgagatc cggaaccctt aatataactt cgtataatgt atgctatacg aagttattag
    10501 gtccctcgaa gaggttcact atgagggagc tgcggtggag gaggtggaga gaaggccgca
    10561 cccttctggg cagggggagg ggagtgccgc aataccttta tgggagttct ctgctgcctc
    10621 ccgtcttgta aggaccgccc tgggcctgga agaagccctc cctcctttcc tcctcgcgtg
    10681 atctcgtcat cgcctccatg tcgagtcgct tctcgattat gggcgggatt cttttgccta
    10741 ggcttaaggg gctaacttgg tccctgggcg ttgccctgca ggggagtgag cagctgtaag
    10801 atttgagggg cgactccgat tagtttatct tcccacggac tagagttggt gtcgaggtta
    10861 ttgtaataag ggtggggtag ggaaatggag cttagtcatt cacctggggc tgattttatg
    10921 caacgagact gcggattatc actaatcatt tttggagcat ttttctagag acagacataa
    10981 agcatgatca cctgagtttt ataccatttg agacccttgc tgcaccacca aagtgtagca
    11041 tcaggttaaa tcttaataga aaaattttag cttttgcttg agaaaccagt gcttccctcc
    11101 ctcaccctct ctccccaggc tctctacccc tttgcatccc taccaggcat cttagcaact
    11161 ctcactcata cttgatccca ttttccattt gttgtacttg ctcctccagt attcagacat
    11221 agcactagct ttctccctct cttgatcttg ggtagcctgg tgtctcgcga aaccagacag
    11281 attggttcca ccacaaatta aggcttgagc tggggcttga ctcttaccca gcagtgcttt
    11341 tattcctccc tagttcacgt tcttaaatgt ttatcttgat tttcatttta tcctttttcc
    11401 ttagctggga ttctgtccct gaccgtcttc acagtccagg tgatcttgac tactgcttta
    11461 cagagaattg gatctgaggt taggcaacat ctcccttttt cttcctctaa atacctctca
    11521 tttctgttct taccagttag taactgatct cagatgcctg tgtgatagct ggtacccagc
    11581 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
    11641 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg gagcataaag
    11701 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
    11761 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
    11821 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
    11881 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
    11941 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
    12001 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
    12061 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
    12121 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
    12181 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
    12241 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
    12301 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
    12361 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
    12421 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
    12481 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
    12541 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
    12601 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
    12661 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
    12721 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
    12781 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
    12841 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
    12901 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
    12961 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
    13021 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
    13081 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
    13141 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
    13201 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    13261 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    13321 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    13381 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    13441 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    13501 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    13561 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    13621 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    13681 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    13741 ataggggttc cgcgcacatt tccccgaaaa gtgccac
//
