LOCUS       sequence-41289-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Lee HJ, Kim E, Kim JS
  TITLE     Targeted chromosomal deletions in human cells using zinc finger 
            nucleases.
  JOURNAL   Genome Res. 2010 Jan;20(1):81-9. Epub 2009 Dec 1.
  PUBMED    19952142
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2010-01"; volume: "20(1)"; pages: "81-9. Epub 2009 Dec
            1"
COMMENT     Sequence Label: p3-K230-FokI_L (wt)
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      81..90
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             93..119
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             93..119
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             129..149
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt gccaccatgg tgtaccccta cgacgtgccc gactacgccg
      121 aattgcctcc aaaaaagaag agaaaggtag ggatccgaat tcccggggaa aaaccgtatg
      181 agtgtgatca ctgtggaaaa tcctttagcc agagctctca tctgaatgtg cacaaaagaa
      241 ctcacaccgg ggaaaaaccg tataaatgcg gccagtgtgg gaagttctac tcgcaggtct
      301 cccacctcac ccgccaccag aaaatccaca ccggggaaaa accgtatgaa tgcgatcact
      361 gtgggaaagc cttcagcgtc agctccaacc tgaacgtgca cagaagaatc cacaccgggg
      421 aaaaaccgta taagtgccct gattgtggga agagttttag tcagagttcc agcctcattc
      481 gccaccagcg gacacacacc ggtgaaaaac aactagtcaa aagtgaactg gaggagaaga
      541 aatctgaact tcgtcataaa ttgaaatatg tgcctcatga atatattgaa ttaattgaaa
      601 ttgccagaaa ttccactcag gatagaattc ttgaaatgaa ggtaatggaa ttttttatga
      661 aagtttatgg atatagaggt aaacatttgg gtggatcaag gaaaccggac ggagcaattt
      721 atactgtcgg atctcctatt gattacggtg tgatcgtgga tactaaagct tatagcggag
      781 gttataatct gccaattggc caagcagatg aaatgcaacg atatgtcgaa gaaaatcaaa
      841 cacgaaacaa acatatcaac cctaatgaat ggtgga
//
