LOCUS       sequence-412895-        8647 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  this mini-donor knockin vector can be used to knock EGFP into SOX17 
            locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8647)
  AUTHORS   Haideri T, Lin J, Bao X, Lian XL
  TITLE     MAGIK: A rapid and efficient method to create lineage-specific 
            reporters in human pluripotent stem cells.
  JOURNAL   Stem Cell Reports. 2024 May 14;19(5):744-757. doi: 
            10.1016/j.stemcr.2024.03.005. Epub 2024 Apr 4.
  PUBMED    38579711
REFERENCE   2  (bases 1 to 8647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2024.03.005"; journalName: "Stem Cell Reports"; 
            date: "2024-05-14- 14"; volume: "19"; issue: "5"; pages: "744-757"
COMMENT     Sequence Label: mini-donor - SOX17-EGFP
FEATURES             Location/Qualifiers
     source          1..8647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      69..497
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(156..175)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     366..387
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             841..1635
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(895..914)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1505..1524
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             join(1653..1721,1722..2513)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1871..1890)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2684..3272
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3173..3192
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3426..3443
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3560..3581
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3596..3601,3602..3619,3620..3626)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3634..3650
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3639..3661
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3658..3674
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3658..3674
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             5414..5431
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             5420..5437
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             5714..5770
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5777..5797
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5801..5803,5804..6517)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5801..5803,5804..6514)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5843..5864)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6104..6123)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6451..6472
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8533..8552)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8534..8552)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8559..8576)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8559..8575)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8568..8590)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
       61 gcgaatggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
      121 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
      181 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
      241 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
      301 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
      361 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
      421 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
      481 aaaatttaac gcgaatttta acaaaattca gggcgcaagg gctgctaaag gaagcggaac
      541 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct
      601 atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca
      661 tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg
      721 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca
      781 aggatctgat ggcgcagggg atcaagatct gatcaagaga caggatgagg atcgtttcgc
      841 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
      901 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
      961 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     1021 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     1081 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     1141 gatctcctgt catcccacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     1201 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     1261 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     1321 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     1381 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     1441 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     1501 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     1561 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     1621 gacgagttct tctgaattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     1681 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     1741 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     1801 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     1861 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     1921 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     1981 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     2041 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     2101 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     2161 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     2221 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     2281 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     2341 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     2401 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     2461 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     2521 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     2581 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     2641 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     2701 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     2761 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     2821 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     2881 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     2941 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     3001 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     3061 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     3121 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     3181 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     3241 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     3301 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     3361 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     3421 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     3481 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     3541 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     3601 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     3661 gaaacagcta tgaccatgat tacgccaagc ttggtaccga gctcggatcc actagtaacg
     3721 gccgccagtg tgctggaatt cgcccttttc ctgcagggca tctcagtgcc tcactcccca
     3781 ccccctcccc cgggtcgggg gaggcggcgc gtccggcgga gggttgaggg gagcggggca
     3841 ggcctggagc gccatgagca gcccggatgc gggatacgcc agtgacgacc agagccagac
     3901 ccagagcgcg ctgcccgcgg tgatggccgg gctgggcccc tgcccctggg ccgagtcgct
     3961 gagccccatc ggggacatga aggtgaaggg cgaggcgccg gcgaacagcg gagcaccggc
     4021 cggggccgcg ggccgagcca agggcgagtc ccgtatccgg cggccgatga acgctttcat
     4081 ggtgtgggct aaggacgagc gcaagcggct ggcgcagcag aatccagacc tgcacaacgc
     4141 cgagttgagc aagatgctgg gtgagtccga gtcgcagacc caggcggccg ggcgcgctgg
     4201 cgcgaatcgc taggccgatt tcttaaaccc caaactgttc tttgcgagcc tgacgcccaa
     4261 aaccaggggt gtgtagcggc cacgtccttt cttaaggctc tgggttccct tcccgcttcc
     4321 cgccctccga ccctccaaag cagctttccg ccttgctctc cggctcccgg attccccagg
     4381 tggccggggg cgcgggtcca acggctctgg gaaggcgact tcccggcacc tccgggcggc
     4441 gcgagagcac ccttggccct gaactgggcc ggttgtgtcc atccctcgac cccttcccta
     4501 gttaggtgtc cttttctgtt tttcgaacga ccgggtgatg ggtgagcgga aagccgcttc
     4561 caggagacca aaagaaaggg gtgcctttag aggacgggtg ttccccaagg gctcggactc
     4621 aggagtccca gatctccctc tttaacttca ccccggttgc gcaattcaaa gtctgagggg
     4681 ggaggtgcgt ccaggtgggg ccaggtgggg cctggagcgg gagcgcagcc gataagccct
     4741 gcgcccctct cccccttcct tccactgtgc aggcaagtcg tggaaggcgc tgacgctggc
     4801 ggagaagcgg cccttcgtgg aggaggcaga gcggctgcgc gtgcagcaca tgcaggacca
     4861 ccccaactac aagtaccggc cgcggcggcg caagcaggtg aagcggctga agcgggtgga
     4921 gggcggcttc ctgcacggcc tggctgagcc gcaggcggcc gcgctgggcc ccgagggcgg
     4981 ccgcgtggcc atggacggcc tgggcctcca gttccccgag cagggcttcc ccgccggccc
     5041 gccgctgctg cctccgcaca tgggcggcca ctaccgcgac tgccagagtc tgggcgcgcc
     5101 tccgctcgac ggctacccgt tgcccacgcc cgacacgtcc ccgctggacg gcgtggaccc
     5161 cgacccggct ttcttcgccg ccccgatgcc cggggactgc ccggcggccg gcacctacag
     5221 ctacgcgcag gtctcggact acgctggccc cccggagcct cccgccggtc ccatgcaccc
     5281 ccgactcggc ccagagcccg cgggtccctc gattccgggc ctcctggcgc cacccagcgc
     5341 ccttcacgtg tactacggcg cgatgggctc gcccggggcg ggcggcgggc gcggcttcca
     5401 gatgcagccg caacaccagc accagcacca gcaccagcac caccccccgg gccccggaca
     5461 gccgtcgccc cctccggagg cactgccctg ccgggacggc acggacccca gtcagcccgc
     5521 cgagctcctc ggggaggtgg accgcacgga atttgaacag tatctgcact tcgtgtgcaa
     5581 gcctgagatg ggcctcccct accaggggca tgactccggt gtgaatctcc ccgacagcca
     5641 cggggccatt tcctcggtgg tgtccgacgc cagctccgcg gtatattact gcaactatcc
     5701 tgacgtggct agcgccacta acttctccct gttgaaacaa gcaggggatg tcgaagagaa
     5761 tcccgggcca atgggcccaa agaagaagag aaaggtttcg gtgagcaagg gcgaggagct
     5821 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     5881 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     5941 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     6001 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     6061 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     6121 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     6181 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     6241 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     6301 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     6361 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     6421 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     6481 cgggatcact ctcggcatgg acgagctgta caagtagggc cgcccctgca ggccagaagc
     6541 agtgttacac acttcctgga ggagctaagg aaatcctcag actcctgggt ttttgttgtt
     6601 gctgttgttg ttttttaaaa ggtgtgttgg catataattt atggtaattt attttgtctg
     6661 ccacttgaac agtttggggg ggtgaggttt catttaaaat ttgttcagag atttgtttcc
     6721 catagttgga ttgtcaaaac cctatttcca agttcaagtt aactagcttt gaatgtgtcc
     6781 caaaacagct tcctccattt cctgaaagtt tattgatcaa agaaatgttg tcctgggtgt
     6841 gttttttcaa tcttctaaaa aataaaatct ggaatcctgc ttttttgctc tactagtacc
     6901 tctgtcacac tagtcttatc aaaaaccagt tcttaagatc aatgttaagt ttattagtta
     6961 atgtaaattt ctcatcctcg aaaagggtga acataaatgc ctttaaggag tatatctaaa
     7021 aataaacatt aggatatcta agtttgatgt aattgtttca ggaaggaaaa aagaaaagca
     7081 ttctggaatg agcctacttc aagtaatctt agtttctaaa actaacagtt aatattttca
     7141 attccagtat atcactttaa gtagaagggg atgtccaagt aattttggtt ttctaactgt
     7201 tgaatcataa gcttgacctg cccccagagg ctttttggat gtttttatct gtgttttgcc
     7261 atctctttac actcctcgac attcagttta ccttaatctt cacattttta caccttggga
     7321 agtggcaagc atcgctgggt ttaagataaa ggagtcacaa aaactaatca aaataaaatt
     7381 tgcattatga caacttttaa tacactttag cattttattt gattggtttt tttttttttt
     7441 tgcttttaga tttaatacat atacggacga ttgcacagac ttaggggaga atccatgaat
     7501 ggtttgtgtg tagtgtccct atctatcacc attactttat caagaagaaa cagttacagg
     7561 gttgtgagta aaagatgggg atcagcccct gtaatctgcc atactaatcg ctgtggcacg
     7621 tctcaccaca tttgagattg agacataaat ggaaacaggt gacttataat gggcatttcc
     7681 tgtgccagat gtttgttcaa tgccattcta aacagtgtga gcctcaacaa acctgcaggt
     7741 ttaggtgcta tttttcctat tttgtaagaa aaggaggcac caggccttcc cctgccatgt
     7801 atatgaaggc ttgtattaca agttcttttg acatatttct cacctgtttt cctctgggcc
     7861 agtatgtatt catgggcact gggagaattt acattctgtt gatgagaaac catataatta
     7921 tccttagcag caagtgctat agaaaaagga aaggcttgga ggatagcttc cagcatttac
     7981 atgggagaga tgtctgagct gaaatgattg gctggattct cttgaaaaag ggcatggaat
     8041 atgtttttta gaagggattc ctggaatgtg aaggatagac agttttagtg aggaaaggta
     8101 agtttattct gtcaggcata acaggctgcc tttaaaaagg ttaaattctt tctcttagct
     8161 cagatgggtt gtttctgctt ccagcctgag ctccttctgc agggtgtccc aagagttccc
     8221 agtagataaa gtggacagaa gggctaccag cccaagttca gggctgtgct cagattacac
     8281 ttggcatttt catcatagtc agcccaagaa gactcagttt tacccttggc tgccatctct
     8341 taacgatatt aagataagta cagaaaagaa aaagaaaaag agttcttagg ggaaagatct
     8401 aaagtcctgt aaattacatc tttaactact cactaggcgc aggtggaaaa gcaggccggc
     8461 caaaagggcg aattctgcag atatccatca cactggcggc cgctcgagca tgcatctaga
     8521 gggcccaatt cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt
     8581 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     8641 gccagct
//
