LOCUS       sequence-412907-       12394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rabbit Cav2.2 calcium channel with a N-terminal EYFP 
            tag and an exofacial double HA tag in domain II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12394)
  AUTHORS   Raghib A, Bertaso F, Davies A, Page KM, Meir A, Bogdanov Y, Dolphin 
            AC
  TITLE     Dominant-negative synthesis suppression of voltage-gated calcium 
            channel Cav2.2 induced by truncated constructs.
  JOURNAL   J Neurosci. 2001 Nov 1;21(21):8495-504.
  PUBMED    11606638
REFERENCE   2  (bases 1 to 12394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "2001-11-1"; volume: "21(21)"; pages: "8495-504"
COMMENT     Sequence Label: EYFP Cav2.2 pRK5
FEATURES             Location/Qualifiers
     source          1..12394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     828..845
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      926..935
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(932..934,935..937,938..1648)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(977..998)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1238..1257)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1585..1606
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      8694..8703
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    8707..8841
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8744..8763)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8798..8817
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        8910..9267
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8910..9101
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8933..8953)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9118..9253
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9287..9304)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9287..9303)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9296..9318)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9516..9971
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9603..9622)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9813..9834
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9988..10007)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10107..10129
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10167..10185)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10253..10357
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10358..10426,10427..11218)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10576..10595)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11389..11977
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11878..11897
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12131..12148
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12265..12286
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12301..12306,12307..12324,12325..12331)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12339..12355
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12344..12366
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12363..12379
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12363..12379
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attccgccac catggtgagc aagggcgagg agctgttcac
      961 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
     1021 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
     1081 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca
     1141 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
     1201 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
     1261 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     1321 cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa
     1381 cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca
     1441 caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg
     1501 cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa
     1561 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     1621 cactctcggc atggacgagc tgtacaagtc cagcatggtc cgcttcgggg acgagctggg
     1681 cggccgctat gggggcgcgg gcggcgcgga gcgggcccgg ggcggcgggg ccggcggggc
     1741 tggcggcccg ggccccgggg ggctgccgcc gggccagcgg gtcctctaca aacagtcgat
     1801 cgcgcagcgc gcgcggacca tggcgctgta caaccccatc ccggtcaagc agaactgctt
     1861 caccgtcaac cgctcgctct tcgtcttcag cgaggacaac gtcgtccgca aatacgccaa
     1921 gcgcatcacc gagtggcctc cgttcgagta catgatcctg gccaccatca tcgccaactg
     1981 catcgtgctg gccctggagc agcacctccc tgacggggac aagacgccta tgtccgagcg
     2041 cctggatgac accgagccct acttcattgg gatcttctgc ttcgaggcgg ggatcaagat
     2101 cctggccctg ggctttgttc tgcacaaggg ctcctatctg cggaacggct ggaacgtcat
     2161 ggacttcgtg gtagtcctca cggggattct tgccacagcc ggaaccgact tcgacctgcg
     2221 aacgctgagg gctgtgcgtg tgctaaggcc cctgaagctg gtgtctggga ttccgagctt
     2281 gcaggtggtg ctcaagtcca tcatgaaggc catggtcccg ctgctgcaga tcgggctgct
     2341 gctcttcttc gccatcctca tgtttgccat catcggcctc gagttctaca tgggcaagtt
     2401 ccacaaggcc tgcttcccca acagcacaga cccagatccc gtgggtgact tcccctgtgg
     2461 caaggaggcc ccggcccggc tctgtgaggg agacacggag tgccgggagt actgggccgg
     2521 acccaacttt ggtatcacca actttgacaa catcctgttt gccatcttga ccgtgttcca
     2581 gtgcatcacc atggagggct ggactgacat cctctacaat acaaacgatg cagccggcaa
     2641 cacgtggaac tggctctact tcatccctct catcatcatc ggctccttct tcatgctcaa
     2701 cctggtgctg ggtgtgctct caggagagtt tgctaaggag cgcgagagag tggagaaccg
     2761 ccgcgccttc ctgaagctcc ggcggcagca gcagattgag cgggagctca acgggtacct
     2821 ggagtggatc ttcaaggctg aggaagtcat gctggctgag gaggacagga acgcagagga
     2881 gaagtcccct ctggatgcag tgttgaagag agcagctgcc aagaagagca gaagcgacct
     2941 gatccaggcc gaggaggggg agggccggct cacaggcctc tgtgctcccg gttccccgtt
     3001 cgcccgtgcc agcctgaaaa gcgggaagac ggagagctcg tcgtacttcc ggaggaagga
     3061 gaagatgttc cgcttcttca tccggcgcat ggtgaaggca cagagcttct actggacggt
     3121 gctgtgtgtg gtggcactga acacactgtg cgtggccatg gtgcactaca accagccgca
     3181 gcggctcacc acggcattgt actttgcaga gtttgttttc ctgggtctct tcctcacaga
     3241 gatgtccctg aagatgtatg gcctggggcc caggagctac ttccggtcct ccttcaactg
     3301 ttttgacttt ggggtcatcg tgggcagtat ctttgaagtg gtctgggctg ctgtcaagcc
     3361 agggacctcc ttcgggatca gtgttctgcg ggctctgcgc ttactgagaa tcttcaaggt
     3421 caccaaatac tggaactccc tacggaacct ggtggtgtct ctgctcaact ccatgaagtc
     3481 catcatcagc ctcctcttcc tgctcttcct gttcattgtt gtctttgctc tgctggggat
     3541 gcagctgttt ggaggccagt tcaactttaa agacgagacc ccaacgacca actttgacac
     3601 cttccctgct gctatcctca ccgtcttcca gatcctgacc ggagaggact ggaatgcggt
     3661 catgtaccac gggatcgagt cgcaaggtgg cgtcagcaga ggaatgttct cctccttcta
     3721 cttcattgtc ctcactctgt ttgggaacta caccctgctg aatgtcttcc tggccattgc
     3781 tgtggacaac cttgccaatg ctcaggagct gaccaaggat gaagaggaga tggaagaagc
     3841 agccaatcag aaactcgcct tgcagaaggc caaggaagtg gctgaagtca gccccatgtc
     3901 tgctgcgaac atctccattg ccgccaggca gcagaactcg gccaaagcgc gctcggtgtg
     3961 ggagcagcga gccagccagc tgcggctgca gaacctgagg gccagctgcg aggcgctgta
     4021 cagcgagatg gaccccgagg agaggctgcg ctacgccacc gcgcgccacc tgcggcccga
     4081 cgtgaagacg cacctggacc ggccgctggt ggttgagccc ggccgcgacg ctccgcgggg
     4141 ccccccgggg gggaagtccc ggccggatgg ctcggaggcc cccgagggtg cggatccccc
     4201 gcgcaggcat caccggcacc gagacaagga caaggctccc gccacggtcc cctcggcggg
     4261 ggaacaggac agagccgaag ctctgagggc agagggcggg gagctggggc cccgcgagga
     4321 gcgggggcgg ccacgccgca gccgcagcaa ggaggccccg ggcgccccgg aggtgcgcag
     4381 cgatcgcggc cgtggcccgt gtcctgaagg tggtcggcgc caccaccggc gcgggtcacc
     4441 ggaggaggca gctgagaggg agccccgccg ccaccgcgcg caccggcacg ggcccgaccc
     4501 gggcaaggag ggcccagcgt ccgggaccag gggcgagcgg cgcgcacgac accgcacggg
     4561 cccccgggcg tgcccccggg aggctgagag cagcgaggag cctgcgcggc ggcaccgggc
     4621 tcggcacaag gcgccgccca cccaggagac cgcggagaag gacaaggagg ctgccgagaa
     4681 ggggggcgag gccacggagg ccgagaagga caaggaggct cggaaccacc agcccaagga
     4741 gctgccctgt gacctggagg ccatcggcat gctgggagtg ggcgctgtgc atacgctgcc
     4801 cagcacctgt ctgcagaagg tggaggagca gccagaggat gcggacaacc agcggaacgt
     4861 cacccgcatg ggtagtcagc ccccggacac cagcaccact gtgcacattc ctgtgacgct
     4921 gaccggcccc cccggggaga ccacggtagt gcccagtgga aacgtggacc tggaaagtca
     4981 agcagagggg aagaaggagg tggaaaccag cgatgtgatg aggagcggcc ctcggcccat
     5041 tgtcccgtac agttccatgt tctgtttaag cccaaccaac ctgctgcgcc gctgctgcca
     5101 ctacattgtg accatgcgat acttcgagat ggtcatcctc gtggtcatcg ccctgagcag
     5161 catcgccctg gctgccgagg accccgtgag aacagactct cccaggaata atgctctgaa
     5221 gtacatggac tacatcttca caggcgtctt caccttcgag atggtgataa agatgatcga
     5281 tttgggactg ctgctacacc ctggagccta tttccgggat ctgtggaaca ttctggactt
     5341 cattgtggtc agtggggccc tggtggcatt tgctttctca ggatccaaag ggaaagacat
     5401 cagcaccatc aaatccctga gagtcctgcg tgtgctgcgg ccactcaaga ccatcaagcg
     5461 gctgcccaag ctcaaggccg tgtttgactg tgtggtgaac tcactgaaaa acgtcctcaa
     5521 catcctgatc gtctacatgc tcttcatgtt catctttgcc gtcattgccg tgcagctctt
     5581 caaagggaag ttcttctact gcacggacga atccaaggag ctggagcgtg actgcagggg
     5641 tcagtatttg gattatgaga aggaggaagt ggaagctcaa ccgaggcagt ggaagaaata
     5701 tgacttccac tatgacaacg tgctctgggc tctgctaacg ctcttcacgg tgtccacagg
     5761 ggaagggtgg cccatggtgc tgaaacactc tgtggatgcc acctacgagg agcagggccc
     5821 gagccccggg taccgcatgg agctctccat cttctacgtg gtctactttg tggtctttcc
     5881 cttcttcttt gtcaacatct tcgtggcctt gatcatcatc accttccagg agcaggggga
     5941 caaggtgatg tccgagtgca gcctggagaa gaacgagagg gcttgcatcg actttgccat
     6001 cagcgccaga cccctgacgc ggtacatgcc ccagaacaag cagtcgttcc agtataagac
     6061 gtggacgttc gtggtgtccc caccttttga gtacttcatc atggctatga tcgccctcaa
     6121 cactgtggtg ctgatgatga agttctacga tgcgccgtac gagtatgagc taatgctgaa
     6181 atgcctgaac atcgtgttca cgtccatgtt ctccatggag tgtgtgctga agatcatcgc
     6241 cttcggggtg ctgaactatt tcagagatgc ctggaatgtc tttgactttg tcacggtgtt
     6301 gggaagtatt actgatattt tggtaacaga gattgcgaac aacttcatca acctcagctt
     6361 cctgcgcctg ttccgggccg cgcggctcat caagctgctg cgccagggct acaccatccg
     6421 catcctgctt tggaccttcg tgcagtcctt caaggcgctg ccctacgtgt gtctcctcat
     6481 cgccatgctc ttcttcatct acgccatcat tggcatgcag gtttttggga acatcgccct
     6541 ggacgatgac accagcatca acaggcacaa taacttccgg acgtttctgc aggccttgat
     6601 gttgctgttc aggagcgcca cgggggaggc ctggcacgag ataatgctgt cgtgcctcag
     6661 cagccgggcc tgcgacgagc actccaatgc cagcgagtgc ggcagcgact ttgcctactt
     6721 ttacttcgtc tccttcatct tcctctgctc ctttctgatg ttgaacctct ttgtggctgt
     6781 gatcatggac aattttgagt accttacacg ggactcttcc atcttagggc ctcaccacct
     6841 ggatgagttc atccgggtct gggctgaata cgacccggcc gcgtgtgggc gcatcagtta
     6901 cagtgacatg tttgagatgc tgaagcacat gtccccaccc ctggggctgg ggaagaagtg
     6961 ccctgctcga gtcgcgtaca agcgcctggt ccgcatgaac atgcccatct ccagtgagga
     7021 catgacggtg cacttcacat ccacgctgat ggctctcatt cggacagcgc tggacatcaa
     7081 gctggcacca gctgggacca agcagcacca gtgcgacgca gagctgagga aggagatttc
     7141 ctgtgtgtgg gccaatctgc cccagaagac cctggactta ctggtaccac cccataagcc
     7201 tgacgagatg acggtgggca aagtctatgc agctctgatg atcttcgact tctacaaaca
     7261 gaacaaaacc agcagagacc agactcagca agcccccgga ggcctgtccc agctgggccc
     7321 cgtgtccctg ttccacccgc tgaaggccac gctggagcag actcagccgg cgctgcgtgg
     7381 agcccgggct ttcctgcggc agaagagttc tgcctccctg agcaacggcg gggccgtaca
     7441 aacccaggag agcggcatca aggaatcggt ttcttggggc actcagagga cccaggatgt
     7501 gctttgcgag gccagggcac ccctggagcg gggccactca gctgagatcc cagtcgggca
     7561 gcctgggaca ctggctgtag atgtccagat gcagaacatg actctgagtg gccctgacgc
     7621 cgagccgcag ccagggctgg agagccaggg ccgagccgcc tccatgcccc gcctggccgc
     7681 agaaacacag cccgcgcctg acgccagccc catgaagcgc tccatctcca cgctggcccc
     7741 gcgcccccac acggcccggc tcggcagcac cgcgctggac cgcccagccc ccagccaggc
     7801 cccgcaccat caccaccacc gctgccaccg ccgcagggac aggaagcagc ggtccctgga
     7861 gaagggtccc agcctgtctg ctgacacaga tggggccccc gacagcacgg tggggccagg
     7921 actgcccacg ggggagggcc cccccggctg caggcgggag cgggagcgca ggcaggagcg
     7981 gggccggtcc caggagcgga ggcagccctc gtcctcctcc tcggagaagc atcgcttcta
     8041 ctcctgtgac cgcttcgggg gccgcgaacc cccacagccc aagccctccc tcagtagtca
     8101 ccccacgtcg ccgacggccg ggcaggagcc gggaccccac ccgcagggga gtggttcggt
     8161 tcacgggagc cccctgctgt ccacatctgg tgctagcacc ccgggccggg ggcggaggca
     8221 gctcccccag acccccttga ccccccgccc cagcgtcacc tacaagacgg ccaactcctc
     8281 gcctgtccac ttcgccgggg caccgagcgg cctccccgcc ttctcccccg gccggctcag
     8341 ccggggcctc tcagaacaca acgccctgct gcagagggac ccgctcagcc ggcccctggc
     8401 gcccggctcg cgcatcggct ccgaccccta cctggggcag cggctggaca gcgaggcgcc
     8461 cgcccgtgcc ctgcccgagg acgcgcccgc ctttgaggag accgcggcca gcaactcggg
     8521 ccgctcctcc cggacctcgt acgtgtcctc actgacctcg cagcccccgc cgctgcgccg
     8581 cgtgcccaac ggctaccact gcactctggg gctgggcggg ggcggccggg cgcggcgcgg
     8641 ctgccaccac cccgaccgcg accgccggtg ctagactagt tctagcaagc ttggccgcca
     8701 tggcccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8761 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8821 gtatcttatc atgtctggat cgggaattaa ttcggcgcag caccatggcc tgaaataacc
     8881 tctgaaagag gaacttggtt aggtaccttc tgaggcggaa agaaccagct gtggaatgtg
     8941 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     9001 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     9061 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     9121 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     9181 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     9241 ttttttggag gcctaggctt ttgcaaaaag ctgttaacag cttggcactg gccgtcgttt
     9301 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     9361 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     9421 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
     9481 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
     9541 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     9601 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     9661 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     9721 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     9781 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     9841 actcaaccct atctcgggct attcttttga tttataaggg attttgccga tttcggccta
     9901 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     9961 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
    10021 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
    10081 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
    10141 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
    10201 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
    10261 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
    10321 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
    10381 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
    10441 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
    10501 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
    10561 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
    10621 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
    10681 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
    10741 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
    10801 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
    10861 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
    10921 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
    10981 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
    11041 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
    11101 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
    11161 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
    11221 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
    11281 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
    11341 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
    11401 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
    11461 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
    11521 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
    11581 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
    11641 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
    11701 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
    11761 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
    11821 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
    11881 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
    11941 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
    12001 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
    12061 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
    12121 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
    12181 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
    12241 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
    12301 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
    12361 cacaggaaac agctatgaca tgattacgaa ttaa
//
