LOCUS       sequence-412910-        5475 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of Cav2.1 N-terminus (amino acids 1-100) with C-terminal 
            CAAX (last 10 aa of H.Ras) motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Page KM, Heblich F, Margas W, Pratt WS, Nieto-Rostro M, Chaggar K, 
            Sandhu K, Davies A, Dolphin AC
  TITLE     N terminus is key to the dominant negative suppression of Ca(V)2 
            calcium channels: implications for episodic ataxia type 2.
  JOURNAL   J Biol Chem. 2010 Jan 8;285(2):835-44. doi: 10.1074/jbc.M109.065045.
            Epub 2009 Nov 10.
  PUBMED    19903821
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.065045"; journalName: "J Biol Chem"; date: 
            "2010-01-8- 8"; volume: "285"; issue: "2"; pages: "835-44"
COMMENT     Sequence Label: An CAAX pMT2
FEATURES             Location/Qualifiers
     source          1..5475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(7..336)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      21..156
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     321..341
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     intron          805..1034
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     1017..1036
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             1530..2093
                     /codon_start=1
                     /gene="Mus musculus Dhfr"
                     /product="mouse dihydrofolate reductase"
                     /label=DHFR
                     /translation="MVRPLNCIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
                     GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
                     ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
                     PGVLSEVQEEKGIKYKFEVYEKKD"
     polyA_signal    2280..2414
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2317..2336)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2371..2390
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3087..3104)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3258..3846)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3338..3357)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4017..4808,4809..4877))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4640..4659
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4878..4982)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5050..5068
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5106..5128)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5228..5247
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5441..5463
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5455..5472
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5456..5472
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
       61 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
      121 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
      181 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
      241 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
      301 ggagcctggg gactttccac accctaactg acacacattc cacaggatcc ggtcgcgcga
      361 atttcgagcg gtgttccgcg gtcctcctcg tatagaaact cggaccactc tgagacgaag
      421 gctcgcgtcc aggccagcac gaaggaggct aagtgggagg ggtagcggtc gttgtccact
      481 agggggtcca ctcgctccag ggtgtgaaga cacatgtcgc cctcttcggc atcaaggaag
      541 gtgattggtt tataggtgta ggccacgtga ccgggtgttc ctgaaggggg gctataaaag
      601 ggggtggggg cgcgttcgtc ctcactctct tccgcatcgc tgtctgcgag ggccagctgt
      661 tgggctcgcg gttgaggaca aactcttcgc ggtctttcca gtactcttgg atcggaaacc
      721 cgtcggcctc cgaacggtac tccgccaccg agggacctga gcgagtccgc atcgaccgga
      781 tcggaaaacc tctcgactgt tggggtgagt actccctctc aaaagcgggc atgacttctg
      841 cgctaagatt gtcagtttcc aaaaacgagg aggatttgat attcacctgg cccgcggtga
      901 tgcctttgag ggtggccgcg tccatctggt cagaaaagac aatctttttg ttgtcaagct
      961 tgaggtgtgg caggcttgag atctggccat acacttgagt gacaatgaca tccactttgc
     1021 ctttctctcc acaggtgtcc actcccaggt ccaactgcag gcgagcctga attgcggccg
     1081 cactagtatg gcccgctttg gagacgagat gccgggccgc tacggcgcag gcggaggagg
     1141 ctcagggccg gccgccgggg tggtcgtggg cgccgcgggc ggccgaggag ccgggggcag
     1201 ccggcagggc gggcagcccg gagcgcagag gatgtacaag cagtcgatgg cgcagagagc
     1261 gcggaccatg gccctctaca accccatccc tgtccgccag aactgcctca cggtcaaccg
     1321 ctccctgttc ctcttcagtg aagacaacgt ggtgagaaaa tacgccaaaa agatcacgga
     1381 atggcctcga ggctgcatga gctgcaagtg tgtgctctcc tgaattccgg gggggggggg
     1441 gggggggaca gctcagggct gcgatttcgc gccaaacttg acggcaatcc tagcgtgaag
     1501 gctggtagga ttttatcccc gctgccatca tggttcgacc attgaactgc atcgtcgccg
     1561 tgtcccaaaa tatggggatt ggcaagaacg gagacctacc ctggcctccg ctcaggaacg
     1621 agttcaagta cttccaaaga atgaccacaa cctcttcagt ggaaggtaaa cagaatctgg
     1681 tgattatggg taggaaaacc tggttctcca ttcctgagaa gaatcgacct ttaaaggaca
     1741 gaattaatat agttctcagt agagaactca aagaaccacc acgaggagct cattttcttg
     1801 ccaaaagttt ggatgatgcc ttaagactta ttgaacaacc ggaattggca agtaaagtag
     1861 acatggtttg gatagtcgga ggcagttctg tttaccagga agccatgaat caaccaggcc
     1921 acctcagact ctttgtgaca aggatcatgc aggaatttga aagtgacacg tttttcccag
     1981 aaattgattt ggggaaatat aaacttctcc cagaataccc aggcgtcctc tctgaggtcc
     2041 aggaggaaaa aggcatcaag tataagtttg aagtctacga gaagaaagac taacaggaag
     2101 atgctttcaa gttctctgct cccctcctaa agctatgcat ttttataaga ccatgggact
     2161 tttgctggct ttagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     2221 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     2281 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     2341 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     2401 atcatgtctg gatccccggc caacggtctg gtgacccggc tgcgagagct cggtgtacct
     2461 gagacgcgag taagcccttg agtcaaagac gtagtcgttg caagtccgca ccaggtactg
     2521 atatcccacc aaaaagtgcg gcggcggctg gcggtagagg ggccagcgta gggtggccgg
     2581 ggctccgggg gcgaggtctt ccaacataag gcgatgatat ccgtagatgt acctggacat
     2641 ccaggtgatg ccggcggcgg tggtggaggc gcgcggaaag tcgcggacgc ggttccagat
     2701 gttgcgcagc ggcaaaaagt gctccatggt cgggacgctc tggccggtga ggcgtgcgca
     2761 gtcgttgacg ctctagaccg tgcaaaagga gagcctgtaa gcgggcactc ttccgtggtc
     2821 tggtggataa attcgcaagg gtatcatggc ggacgaccgg ggttcgaacc ccggatccgg
     2881 ccgtccgccg tgatccatgc ggttaccgcc cgcgtgtcga acccaggtgt gcgacgtcag
     2941 acaacggggg agcgctcctt ttggcttcct tccaggcgcg gcggctgctg cgctagcttt
     3001 tttggcgagc tcgaattaat tctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     3061 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     3121 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     3181 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3241 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3301 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3361 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     3421 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     3481 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3541 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3601 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3661 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     3721 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3781 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     3841 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     3901 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     3961 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4021 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4081 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4141 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     4201 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4261 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     4321 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     4381 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     4441 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     4501 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     4561 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     4621 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     4681 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     4741 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     4801 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     4861 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     4921 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     4981 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     5041 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     5101 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     5161 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     5221 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     5281 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
     5341 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     5401 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     5461 acgacggcca gtgcc
//
