LOCUS       sequence-41294-e         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Lee HJ, Kim E, Kim JS
  TITLE     Targeted chromosomal deletions in human cells using zinc finger 
            nucleases.
  JOURNAL   Genome Res. 2010 Jan;20(1):81-9. Epub 2009 Dec 1.
  PUBMED    19952142
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2010-01"; volume: "20(1)"; pages: "81-9. Epub 2009 Dec
            1"
COMMENT     Sequence Label: p3-K230-FokI_R (RR)
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     49..68
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        49..67
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      81..90
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             93..119
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             93..119
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             129..149
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
       61 ctatagggag acccaagctt gccaccatgg tgtaccccta cgacgtgccc gactacgccg
      121 aattgcctcc aaaaaagaag agaaaggtag ggatccgaat tcccggggaa aaaccgtaca
      181 ggtgtaagta ctgcgaccgc tccttcagcg actcttcgaa cctccagcgg cacgtccgga
      241 acatccacac cggggaaaaa ccgtataaat gcggccagtg tgggaagttc tactcgcagg
      301 tctcccacct cacccgccac cagaaaatcc acaccgggga aaaaccgttt cattgtggtt
      361 attgtgaaaa atctttttct gttaaagatt atttgactaa acatattcgt actcataccg
      421 gggaaaaacc gtataagtgc atggagtgtg ggaaggcttt taaccgcagg tcacacctca
      481 cacggcacca gcggattcac accggtgaaa aacaactagt caaaagtgaa ctggaggaga
      541 agaaatctga acttcgtcat aaattgaaat atgtgcctca tgaatatatt gaattaattg
      601 aaattgccag aaatcccact caggatagaa ttcttgaaat gaaggtaatg gaatttttta
      661 tgaaagttta tggatataga ggtgagcatt tgggtggatc aaggaaaccg gacggagcaa
      721 tttatactgt cggatctcct attgattacg gtgtgatcgt ggatactaaa gcttatagcg
      781 gaggttataa tctgccaatt ggccaagcac gagaaatgca acgatatgtc gaa
//
