LOCUS       sequence-412945-       11254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox Inducible Expression Plasmid for gal4DBD-miniVPR-HA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11254)
  AUTHORS   Allen TP, Roennfeldt AE, Reckdharajkumar M, Liu M, Quinn RJ, Russell
            DL, Peet DJ, Whitelaw ML, Bersten DC
  TITLE     dFLASH; dual FLuorescent transcription factor Activity Sensor for 
            Histone integrated live-cell reporting and high-content screening
  JOURNAL   bioRxiv 2023.11.21.568191
REFERENCE   2  (bases 1 to 11254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.11.21.568191"
COMMENT     Sequence Label: pLV-TET2Puro-gal4DBD-miniVPR-HA
FEATURES             Location/Qualifiers
     source          1..11254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    159..180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(195..200,201..218,219..225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    233..249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     238..260
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     257..273
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     257..273
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     292..312
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        294..312
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        340..566
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             567..747
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    794..919
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1412..1645
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1830..1874
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2136..2253
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2266..2644
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    2274..2292
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2310..2328
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2346..2364
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2382..2400
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2418..2436
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2454..2472
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2490..2508
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2553..2577
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2686..2710
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2784..3224
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             3288..3308
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3363..3512
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             3537..3557
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3717..4073
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             4302..4328
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(4355..4379)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4513..5699
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4744..5690
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5647..5667
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    5772..5796
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5842..6582
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLP"
     CDS             6628..7230
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7127..7147
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8463..8696
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8768..8889
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8805..8824)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8859..8878
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8991..9010
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(9275..9293)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9361..9465
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9466..9534,9535..10326)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9684..9703)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10497..11085
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10986..11005
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgataaccct aattcgatag catatgcttc ccgttgggta acatatgcta ttgaattagg
       61 gttagtctgg atagtatata ctactacccg ggaagcatat gctacccgtt tagggttcac
      121 cggtgatgcc ggccacgatg cgtccggcgt agaggatcta atgtgagtta gctcactcat
      181 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      241 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
      301 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
      361 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
      421 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
      481 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
      541 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
      601 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      661 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      721 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
      781 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
      841 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
      901 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
      961 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     1021 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     1081 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1141 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     1201 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     1261 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1321 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1381 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1441 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1501 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1561 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1621 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1681 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     1741 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1801 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     1861 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     1921 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     1981 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2041 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2101 ccattcgatt agtgaacgga tctcgacggt atcggtttta aaagaaaagg ggggattggg
     2161 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     2221 ttacaaaaac aaattacaaa aattcaaaat tttatcgata ccgtcgagtt tactccctat
     2281 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac
     2341 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga
     2401 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat
     2461 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtataagctt
     2521 taggcgtgta cggtgggcgc ctataaaagc agagctcgtt tagtgaaccg tcagatcgcc
     2581 tggagcaatt ccacaacact tttgtcttat accaactttc cgtaccactt cctaccctcg
     2641 taaagtcgac accggggccc agatctatcg atcggccgga tatcaacaag tttgtacaaa
     2701 aaagcaggct ttaaaggaac caattcagtc gactggatcc ggtaccgagc tcggatctaa
     2761 gcttgaagca agcctcctga aagatgaagc tactgtcttc tatcgaacaa gcatgcgata
     2821 tttgccgact taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga
     2881 agaacaactg ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac
     2941 atctgacaga agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc
     3001 ctcgagaaga ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt
     3061 taacaggatt atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt
     3121 cagtggagac tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat
     3181 cggaagagag tagtaacaaa ggtcaaagac agttgactgt atcgccggaa ttcccgggga
     3241 tccgtcgaga ggcctcgtcg actggatccg gtaccagcag ggctgacccc aagaagaaga
     3301 ggaaggtgag tggtggagga agtggcgggt cagggtcgga ggccagcggt tccggacggg
     3361 ctgacgcatt ggacgatttt gatctggata tgctgggaag tgacgccctc gatgattttg
     3421 accttgacat gcttggttcg gatgcccttg atgactttga cctcgacatg ctcggcagtg
     3481 acgcccttga tgatttcgac ctggacatgc tgattaactc tagaagttcc ggatctccga
     3541 aaaagaaacg caaagttggt tcgggaggtg gttcgggtgg ctctggatca gtgctgcctc
     3601 aggctcctgc tcctgcacca gctccagcca tggtgtctgc actggctcag gcaccagcac
     3661 ccgtgcctgt gctggctcct ggacctccac aggctgtggc tccaccagcc cctaaaccta
     3721 cacaggccgg cgagggcaca ctgtctgaag ctctgctgca gctgcagttc gacgacgagg
     3781 atctgggagc cctgctggga aacagcaccg atcctgccgt gttcaccgac ctggccagcg
     3841 tggacaacag cgagttccag cagctgctga accagggcat ccctgtggcc cctcacacca
     3901 ccgagcccat gctgatggaa taccccgagg ccatcacccg gctcgtgaca ggcgctcaga
     3961 ggcctcctga tccagctcct gcccctctgg gagcaccagg cctgcctaat ggactgctgt
     4021 ctggcgacga ggacttcagc tctatcgccg atatggattt ctcagccttg ctgtcaggcg
     4081 gtggtagtgg tgggagcggt agtgaccttt cccatccgcc cccaaggggc catctggatg
     4141 agctgacaac cacacttgag tccatgaccg aggatctgaa cctggactca cccctgaccc
     4201 cggaattgaa cgagattctg gataccttcc tgaacgacga gtgcctcttg catgccatgc
     4261 atatcagcac aggactgtcc atcttcgaca catctctgtt ttacccatac gatgttccag
     4321 attacgcttg agcggccgca ctcgagatat ctagacccag ctttcttgta caaagtggtt
     4381 gatatcacgc gtaccgtcga cctcgactga tcgggagatc tcgaccgagc tttgcaaaga
     4441 tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa
     4501 ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     4561 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
     4621 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     4681 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     4741 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
     4801 gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
     4861 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
     4921 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
     4981 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
     5041 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atcgatctgc acactggtat
     5101 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc
     5161 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg
     5221 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct
     5281 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg
     5341 gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag
     5401 gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc
     5461 gtccaggcac ctcgattagt tctcgatcga gcttttggag tacgtcgtct ttaggttggg
     5521 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc
     5581 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt
     5641 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag
     5701 gaattagctt gaccaagctt ggtaccgagc tcggatccac tagtaacggc cgccagtgtg
     5761 ctggaattat cacaagtttg tacaaaaaag caggctttaa aggaaccaat tcagtcgact
     5821 ggatccggta ccgaattcac catgtctaga ctggacaaga gcaaagtcat aaactctgct
     5881 ctggaattac tcaatggagt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag
     5941 ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat
     6001 gccctgccaa tcgagatgct ggacaggcat catacccact cctgccccct ggaaggcgag
     6061 tcatggcaag actttctgcg gaacaacgcc aagtcatacc gctgtgctct cctctcacat
     6121 cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg
     6181 gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct
     6241 ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga gcatcaagta
     6301 gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct gaaacaagca
     6361 attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct ggaactaatc
     6421 atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccgaccga cgcccttgac
     6481 gattttgact tagacatgct cccagccgat gcccttgacg actttgacct tgatatgctg
     6541 cctgctgacg ctcttgacga ttttgacctt gacatgctcc ccagggccac gaagcaagca
     6601 ggagatgttg aagaaaaccc cgggcctatg ggcaccgagt acaagcccac ggtgcgcctc
     6661 gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac
     6721 cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac cgagctgcaa
     6781 gaactattcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     6841 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     6901 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     6961 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     7021 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     7081 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     7141 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     7201 tggtgcatga cccgcaagcc cggtgcctga gctagcctag tgccatttgt tcagtggttc
     7261 gtagggcttt cccccactgt ttggctttca gttatatgga tgatgtggta ttgggggcca
     7321 agtctgtaca gcatcttgag tcccttttta ccgctgttac caattttctt ttgtctttgg
     7381 gtatacattt aaaccctaac aaaacaaaga gatggggtta ctctctaaat tttatgggtt
     7441 atgtcattgg atgttatggg tccttgccac aagaacacat catacaaaaa atcaaagaat
     7501 gttttagaaa acttcctatt aacaggccta ttgattggaa agtatgtcaa cgaattgtgg
     7561 gtcttttggg ttttgctgcc ccttttacac aatgtggtta tcctgcgttg atgcctttgt
     7621 atgcatgtat tcaatctaag caggctttca ctttctcgcc aacttacaag gcctttctgt
     7681 gtaaacaata cctgaacctt taccccgttg cccggcaacg gccaggtctg tgccaagtgt
     7741 ttgctgacgc aacccccact ggctggggct tggtcatggg ccatcagcgc atgcgtggaa
     7801 ccttttcggc tcctctgccg atccatactg cggaactcct agccgcttgt tttgctcgca
     7861 gcaggtctgg agcaaacatt atcgggactg ataactctgt tgtcctatcc cgcaaatata
     7921 catcgtttcc atggctgcta ggctgtgctg ccaactggat cctgcgcggg acgtcctttg
     7981 tttacgtccc gtcggcgctg aatcctgcgg acgacccttc tcggggtcgc ttgggactct
     8041 ctcgtcccct tctccgtctg ccgttccgac cgaccacggg gcgcacctct ctttacgcgg
     8101 actccccgtc tgtgccttct catctgccgg accgtgtgca cttcgcttca cctctgcacg
     8161 tcgcatggag accaccgtga acgcccacca aatattgccc aaggtcttac ataagaggac
     8221 tcttggactc tcagcaatgt caacgaccga ccttgaggca tacttcaaag actgtttgtt
     8281 taaagactgg gaggagttgg gggaggagat taggttaaag gtctttgtac taggaggctg
     8341 taggcataaa ttggtctgcg caccagcacc atggcgcaat cactagagcg gggtaccttt
     8401 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     8461 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     8521 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     8581 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     8641 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     8701 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     8761 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8821 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8881 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     8941 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     9001 gaggccgagg ccggatccct tgagtggctt tcatcctgga gcagactttg cagtctgtgg
     9061 actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac accaatgctg
     9121 ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca acgtcaatca
     9181 gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca atagtgttta
     9241 taaggccccc ttgttaattc ttgaagacga aagggcctcg tgatacgcct atttttatag
     9301 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     9361 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     9421 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     9481 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     9541 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     9601 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     9661 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     9721 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     9781 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     9841 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     9901 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     9961 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
    10021 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
    10081 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
    10141 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
    10201 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
    10261 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
    10321 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
    10381 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
    10441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
    10501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
    10561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
    10621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
    10681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
    10741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
    10801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
    10861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    10921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    10981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    11041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    11101 gcctttttac ggttcctggc cttttgctgg ccttgaagct gtccctgatg gtcgtcatct
    11161 acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga
    11221 atcataatgg ggaaggccat ccagcctcgc gtcg
//
