LOCUS       sequence-412947-       10499 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox Inducible Expression Plasmid for gal4DBD-HIF-CAD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10499)
  AUTHORS   Allen TP, Roennfeldt AE, Reckdharajkumar M, Liu M, Quinn RJ, Russell
            DL, Peet DJ, Whitelaw ML, Bersten DC
  TITLE     dFLASH; dual FLuorescent transcription factor Activity Sensor for 
            Histone integrated live-cell reporting and high-content screening
  JOURNAL   bioRxiv 2023.11.21.568191
REFERENCE   2  (bases 1 to 10499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.11.21.568191"
COMMENT     Sequence Label: pLV-tet2puro-gal4DBD-HIFCAD
FEATURES             Location/Qualifiers
     source          1..10499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    159..180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(195..200,201..218,219..225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    233..249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     238..260
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     257..273
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     257..273
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     292..312
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        294..312
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        340..566
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             567..747
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    794..919
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1412..1645
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1830..1874
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2136..2253
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2266..2644
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    2274..2292
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2310..2328
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2346..2364
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2382..2400
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2418..2436
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2454..2472
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2490..2508
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2553..2577
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2686..2710
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2784..3224
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             3243..3272
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(3600..3624)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        3758..4944
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3989..4935
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4892..4912
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    5017..5041
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5087..5827
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLP"
     CDS             5873..6475
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6372..6392
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7708..7941
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8013..8134
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8050..8069)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8104..8123
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8236..8255
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(8520..8538)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8606..8710
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8711..8779,8780..9571)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8929..8948)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9742..10330
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10231..10250
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgataaccct aattcgatag catatgcttc ccgttgggta acatatgcta ttgaattagg
       61 gttagtctgg atagtatata ctactacccg ggaagcatat gctacccgtt tagggttcac
      121 cggtgatgcc ggccacgatg cgtccggcgt agaggatcta atgtgagtta gctcactcat
      181 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      241 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
      301 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
      361 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
      421 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
      481 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
      541 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
      601 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      661 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      721 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
      781 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
      841 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
      901 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
      961 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     1021 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     1081 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1141 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     1201 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     1261 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1321 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1381 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1441 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1501 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1561 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1621 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1681 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     1741 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1801 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     1861 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     1921 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     1981 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2041 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2101 ccattcgatt agtgaacgga tctcgacggt atcggtttta aaagaaaagg ggggattggg
     2161 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     2221 ttacaaaaac aaattacaaa aattcaaaat tttatcgata ccgtcgagtt tactccctat
     2281 cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac gtatgcagac
     2341 tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca gtgatagaga
     2401 acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt tactccctat
     2461 cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac gtataagctt
     2521 taggcgtgta cggtgggcgc ctataaaagc agagctcgtt tagtgaaccg tcagatcgcc
     2581 tggagcaatt ccacaacact tttgtcttat accaactttc cgtaccactt cctaccctcg
     2641 taaagtcgac accggggccc agatctatcg atcggccgga tatcaacaag tttgtacaaa
     2701 aaagcaggct ttaaaggaac caattcagtc gactggatcc ggtaccgagc tcggatctaa
     2761 gcttgaagca agcctcctga aagatgaagc tactgtcttc tatcgaacaa gcatgcgata
     2821 tttgccgact taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga
     2881 agaacaactg ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac
     2941 atctgacaga agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc
     3001 ctcgagaaga ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt
     3061 taacaggatt atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt
     3121 cagtggagac tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat
     3181 cggaagagag tagtaacaaa ggtcaaagac agttgactgt atcggaattc gttatccgac
     3241 cagaacaaaa gttgatttct gaagaagatt tgtcactttt tcaagcagta ggaattggaa
     3301 cattattaca gcagccagac gatcatgcag ctactacatc actttcttgg aaacgtgtaa
     3361 aaggatgcaa atctagtgaa cagaatggaa tggagcaaaa gacaattatt ttaataccct
     3421 ctgatttagc atgtagactg ctggggcaat caatggatga aagtggatta ccacagctga
     3481 ccagttatga ttgtgaagtt aatgctccta tacaaggcag cagaaactta ctgcagggtg
     3541 aagaattact cagagctttg gatcaagtta actgacgcgg ccgcactcga gatatctaga
     3601 cccagctttc ttgtacaaag tggttgatat cacgcgtacc gtcgacctcg actgatcggg
     3661 agatctcgac cgagctttgc aaagatggat aaagttttaa acagagagga atctttgcag
     3721 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
     3781 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
     3841 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     3901 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     3961 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
     4021 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
     4081 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
     4141 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
     4201 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
     4261 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
     4321 ccaagatcga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     4381 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     4441 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     4501 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     4561 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     4621 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     4681 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg atcgagcttt
     4741 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     4801 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     4861 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     4921 tttcttccat ttcaggtgtc gtgaggaatt agcttgacca agcttggtac cgagctcgga
     4981 tccactagta acggccgcca gtgtgctgga attatcacaa gtttgtacaa aaaagcaggc
     5041 tttaaaggaa ccaattcagt cgactggatc cggtaccgaa ttcaccatgt ctagactgga
     5101 caagagcaaa gtcataaact ctgctctgga attactcaat ggagtcggta tcgaaggcct
     5161 gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt
     5221 gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca ggcatcatac
     5281 ccactcctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc
     5341 ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc
     5401 aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt
     5461 ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt
     5521 attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc
     5581 tatgccccca cttctgaaac aagcaattga gctgttcgac cggcagggag ccgaacctgc
     5641 cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa agtgcgaaag
     5701 cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag ccgatgccct
     5761 tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg accttgacat
     5821 gctccccagg gccacgaagc aagcaggaga tgttgaagaa aaccccgggc ctatgggcac
     5881 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac
     5941 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca
     6001 catcgagcgg gtcaccgagc tgcaagaact attcctcacg cgcgtcgggc tcgacatcgg
     6061 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     6121 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     6181 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     6241 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     6301 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     6361 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     6421 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgagctag
     6481 cctagtgcca tttgttcagt ggttcgtagg gctttccccc actgtttggc tttcagttat
     6541 atggatgatg tggtattggg ggccaagtct gtacagcatc ttgagtccct ttttaccgct
     6601 gttaccaatt ttcttttgtc tttgggtata catttaaacc ctaacaaaac aaagagatgg
     6661 ggttactctc taaattttat gggttatgtc attggatgtt atgggtcctt gccacaagaa
     6721 cacatcatac aaaaaatcaa agaatgtttt agaaaacttc ctattaacag gcctattgat
     6781 tggaaagtat gtcaacgaat tgtgggtctt ttgggttttg ctgccccttt tacacaatgt
     6841 ggttatcctg cgttgatgcc tttgtatgca tgtattcaat ctaagcaggc tttcactttc
     6901 tcgccaactt acaaggcctt tctgtgtaaa caatacctga acctttaccc cgttgcccgg
     6961 caacggccag gtctgtgcca agtgtttgct gacgcaaccc ccactggctg gggcttggtc
     7021 atgggccatc agcgcatgcg tggaaccttt tcggctcctc tgccgatcca tactgcggaa
     7081 ctcctagccg cttgttttgc tcgcagcagg tctggagcaa acattatcgg gactgataac
     7141 tctgttgtcc tatcccgcaa atatacatcg tttccatggc tgctaggctg tgctgccaac
     7201 tggatcctgc gcgggacgtc ctttgtttac gtcccgtcgg cgctgaatcc tgcggacgac
     7261 ccttctcggg gtcgcttggg actctctcgt ccccttctcc gtctgccgtt ccgaccgacc
     7321 acggggcgca cctctcttta cgcggactcc ccgtctgtgc cttctcatct gccggaccgt
     7381 gtgcacttcg cttcacctct gcacgtcgca tggagaccac cgtgaacgcc caccaaatat
     7441 tgcccaaggt cttacataag aggactcttg gactctcagc aatgtcaacg accgaccttg
     7501 aggcatactt caaagactgt ttgtttaaag actgggagga gttgggggag gagattaggt
     7561 taaaggtctt tgtactagga ggctgtaggc ataaattggt ctgcgcacca gcaccatggc
     7621 gcaatcacta gagcggggta cctttaagac caatgactta caaggcagct gtagatctta
     7681 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     7741 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     7801 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7861 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7921 cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag tatttataac
     7981 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt
     8041 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     8101 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc
     8161 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     8221 ctgactaatt ttttttattt atgcagaggc cgaggccgga tcccttgagt ggctttcatc
     8281 ctggagcaga ctttgcagtc tgtggactgc aacacaacat tgcctttatg tgtaactctt
     8341 ggctgaagct cttacaccaa tgctggggga catgtacctc ccaggggccc aggaagacta
     8401 cgggaggcta caccaacgtc aatcagaggg gcctgtgtag ctaccgataa gcggaccctc
     8461 aagagggcat tagcaatagt gtttataagg cccccttgtt aattcttgaa gacgaaaggg
     8521 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     8581 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     8641 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     8701 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     8761 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     8821 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     8881 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     8941 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     9001 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     9061 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     9121 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     9181 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     9241 caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     9301 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     9361 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     9421 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     9481 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     9541 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     9601 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     9661 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     9721 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     9781 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     9841 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     9901 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9961 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
    10021 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
    10081 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
    10141 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
    10201 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
    10261 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
    10321 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttg
    10381 aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat
    10441 cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcg
//
