LOCUS       sequence-412949-       10597 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  hypoxic response element driven NanoLuc Lentiviral reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10597)
  AUTHORS   Allen TP, Roennfeldt AE, Reckdharajkumar M, Liu M, Quinn RJ, Russell
            DL, Peet DJ, Whitelaw ML, Bersten DC
  TITLE     dFLASH; dual FLuorescent transcription factor Activity Sensor for 
            Histone integrated live-cell reporting and high-content screening
  JOURNAL   bioRxiv 2023.11.21.568191
REFERENCE   2  (bases 1 to 10597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.11.21.568191"
COMMENT     Sequence Label: pLV-REPORT (PGK) 12xHRE-NanoLuc
FEATURES             Location/Qualifiers
     source          1..10597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    159..180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(195..200,201..218,219..225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    233..249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     238..260
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     257..273
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     257..273
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     292..312
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        294..312
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        340..566
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             567..747
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    794..919
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1412..1645
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1830..1874
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2136..2253
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     3295..3319
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3428..3940
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             3944..4063
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     primer_bind     complement(4111..4134)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4461..4480
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      4546..4555
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4567..4587
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4591..4611
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4615..4635
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(4645..4647,4648..4650,4651..5361,5362..5367,
                     5368..5487)
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(4690..4711)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4951..4970)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5298..5319
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5488..5547
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             5548..6573
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5548..6573
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             7806..8039
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8111..8232
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8148..8167)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8202..8221
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8334..8353
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(8618..8636)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8704..8808
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8809..8877,8878..9669)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9027..9046)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9840..10428
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10329..10348
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgataaccct aattcgatag catatgcttc ccgttgggta acatatgcta ttgaattagg
       61 gttagtctgg atagtatata ctactacccg ggaagcatat gctacccgtt tagggttcac
      121 cggtgatgcc ggccacgatg cgtccggcgt agaggatcta atgtgagtta gctcactcat
      181 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      241 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
      301 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
      361 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
      421 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
      481 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
      541 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
      601 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      661 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      721 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
      781 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
      841 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
      901 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
      961 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     1021 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     1081 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1141 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     1201 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     1261 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1321 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1381 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1441 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1501 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1561 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1621 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1681 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     1741 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1801 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     1861 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     1921 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     1981 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2041 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2101 ccattcgatt agtgaacgga tctcgacggt atcggtttta aaagaaaagg ggggattggg
     2161 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     2221 ttacaaaaac aaattacaaa aattcaaaat tttatcgatt tcgaacctag aggacgtgac
     2281 aaacagaagc cacacgtcct agggacgtgg ggagtgcgtg aggagtacgt gaggacacgt
     2341 gggtactaga ggacgtgaca aacagaagcc acacgtccta gggacgtggg gagtgcgtga
     2401 ggagtacgtg aggacacgtg ggtactagag gacgtgacaa acagaagcca cacgtcctag
     2461 ggacgtgggg agtgcgtgag gagtacgtga ggacacgtgg gtactagagg acgtgacaaa
     2521 cagaagccac acgtcctagg gacgtgggga gtgcgtgagg agtacgtgag gacacgtggg
     2581 tactagagga cgtgacaaac agaagccaca cgtcctaggg acgtggggag tgcgtgagga
     2641 gtacgtgagg acacgtgggt actagaggac gtgacaaaca gaagccacac gtcctaggga
     2701 cgtggggagt gcgtgaggag tacgtgagga cacgtgggta ctagaggacg tgacaaacag
     2761 aagccacacg tcctagggac gtggggagtg cgtgaggagt acgtgaggac acgtgggtac
     2821 tagaggacgt gacaaacaga agccacacgt cctagggacg tggggagtgc gtgaggagta
     2881 cgtgaggaca cgtgggtact agaggacgtg acaaacagaa gccacacgtc ctagggacgt
     2941 ggggagtgcg tgaggagtac gtgaggacac gtgggtacta gaggacgtga caaacagaag
     3001 ccacacgtcc tagggacgtg gggagtgcgt gaggagtacg tgaggacacg tgggtactag
     3061 aggacgtgac aaacagaagc cacacgtcct agggacgtgg ggagtgcgtg aggagtacgt
     3121 gaggacacgt gggtactaga ggacgtgaca aacagaagcc acacgtccta gggacgtggg
     3181 gagtgcgtga ggagtacgtg aggacacgtg ggtactagag gagcatgccc atatcactag
     3241 ggtttaaacg gcgcgccagc tttaggcgtg tacggtgggc gcctataaaa gcagagctcg
     3301 tttagtgaac cgtcagatcg cctggagcaa ttccacaaca cttttgtctt ataccaactt
     3361 tccgtaccac ttcctaccct cgtaaagtcg acaccggggc ccagatctat cgatcggccg
     3421 gatcaccatg gtcttcacac tcgaagattt cgttggggac tggcgacaga cagccggcta
     3481 caacctggac caagtccttg aacagggagg tgtgtccagt ttgtttcaga atctcggggt
     3541 gtccgtaact ccgatccaaa ggattgtcct gagcggtgaa aatgggctga agatcgacat
     3601 ccatgtcatc atcccgtatg aaggtctgag cggcgaccaa atgggccaga tcgaaaaaat
     3661 ttttaaggtg gtgtaccctg tggatgatca tcactttaag gtgatcctgc actatggcac
     3721 actggtaatc gacggggtta cgccgaacat gatcgactat ttcggacggc cgtatgaagg
     3781 catcgccgtg ttcgacggca aaaagatcac tgtaacaggg accctgtgga acggcaacaa
     3841 aattatcgac gagcgcctga tcaaccccga cggctccctg ctgttccgag taaccatcaa
     3901 cggagtgacc ggctggcggc tgtgcgaacg cattctggcg aattctcacg gctttccgcc
     3961 tgaggttgaa gagcaagccg ccggtacatt gcctatgtcc tgcgcacaag aaagcggtat
     4021 ggaccggcac ccagccgctt gtgcttcagc tcgcatcaac gtctaaaatt ctaccgggta
     4081 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
     4141 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
     4201 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
     4261 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
     4321 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
     4381 agcggccaat agcagctttg ctccttcgct ttctgggctc aggggcgggg cgggcgcccg
     4441 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt
     4501 ctcctcttcc tcatctccgg gcctttcgac ctgcatctcg aggtcgccac catggtgcac
     4561 gtggatccaa aaaagaagag aaaggtagat ccaaaaaaga agagaaaggt agatccaaaa
     4621 aagaagagaa aggtacacgt gtccatggtg agcaagggcg aggagctgtt caccggggtg
     4681 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     4741 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     4801 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     4861 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     4921 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     4981 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     5041 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     5101 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     5161 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     5221 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     5281 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     5341 ggcatggacg agctgtacaa gaagcttagc catggcttcc cgccggaggt ggaggagcag
     5401 gatgatggca cgctgcccat gtcttgtgcc caggagagcg ggatggaccg tcaccctgca
     5461 gcctgtgctt ctgctaggat caatgtgcag tgtactaatt atgctctctt gaaattggct
     5521 ggagatgttg agagcaaccc aggtcccatg aaaaagcctg aactcaccgc gacgtctgtc
     5581 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
     5641 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
     5701 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
     5761 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc
     5821 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
     5881 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc
     5941 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
     6001 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
     6061 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc
     6121 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
     6181 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
     6241 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
     6301 aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt
     6361 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
     6421 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
     6481 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
     6541 cgccccagca ctcgtccgag ggcaaaggaa taggctagcc tagtgccatt tgttcagtgg
     6601 ttcgtagggc tttcccccac tgtttggctt tcagttatat ggatgatgtg gtattggggg
     6661 ccaagtctgt acagcatctt gagtcccttt ttaccgctgt taccaatttt cttttgtctt
     6721 tgggtataca tttaaaccct aacaaaacaa agagatgggg ttactctcta aattttatgg
     6781 gttatgtcat tggatgttat gggtccttgc cacaagaaca catcatacaa aaaatcaaag
     6841 aatgttttag aaaacttcct attaacaggc ctattgattg gaaagtatgt caacgaattg
     6901 tgggtctttt gggttttgct gcccctttta cacaatgtgg ttatcctgcg ttgatgcctt
     6961 tgtatgcatg tattcaatct aagcaggctt tcactttctc gccaacttac aaggcctttc
     7021 tgtgtaaaca atacctgaac ctttaccccg ttgcccggca acggccaggt ctgtgccaag
     7081 tgtttgctga cgcaaccccc actggctggg gcttggtcat gggccatcag cgcatgcgtg
     7141 gaaccttttc ggctcctctg ccgatccata ctgcggaact cctagccgct tgttttgctc
     7201 gcagcaggtc tggagcaaac attatcggga ctgataactc tgttgtccta tcccgcaaat
     7261 atacatcgtt tccatggctg ctaggctgtg ctgccaactg gatcctgcgc gggacgtcct
     7321 ttgtttacgt cccgtcggcg ctgaatcctg cggacgaccc ttctcggggt cgcttgggac
     7381 tctctcgtcc ccttctccgt ctgccgttcc gaccgaccac ggggcgcacc tctctttacg
     7441 cggactcccc gtctgtgcct tctcatctgc cggaccgtgt gcacttcgct tcacctctgc
     7501 acgtcgcatg gagaccaccg tgaacgccca ccaaatattg cccaaggtct tacataagag
     7561 gactcttgga ctctcagcaa tgtcaacgac cgaccttgag gcatacttca aagactgttt
     7621 gtttaaagac tgggaggagt tgggggagga gattaggtta aaggtctttg tactaggagg
     7681 ctgtaggcat aaattggtct gcgcaccagc accatggcgc aatcactaga gcggggtacc
     7741 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     7801 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     7861 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7921 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7981 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     8041 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     8101 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     8161 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     8221 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     8281 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     8341 gcagaggccg aggccggatc ccttgagtgg ctttcatcct ggagcagact ttgcagtctg
     8401 tggactgcaa cacaacattg cctttatgtg taactcttgg ctgaagctct tacaccaatg
     8461 ctgggggaca tgtacctccc aggggcccag gaagactacg ggaggctaca ccaacgtcaa
     8521 tcagaggggc ctgtgtagct accgataagc ggaccctcaa gagggcatta gcaatagtgt
     8581 ttataaggcc cccttgttaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     8641 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     8701 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     8761 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     8821 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     8881 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     8941 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     9001 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     9061 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     9121 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     9181 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     9241 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     9301 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     9361 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     9421 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     9481 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     9541 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     9601 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     9661 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     9721 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     9781 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     9841 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     9901 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     9961 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
    10021 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
    10081 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
    10141 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
    10201 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
    10261 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
    10321 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
    10381 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
    10441 gcggcctttt tacggttcct ggccttttgc tggccttgaa gctgtccctg atggtcgtca
    10501 tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga
    10561 agaatcataa tggggaaggc catccagcct cgcgtcg
//
