LOCUS       sequence-41295-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Lee HJ, Kim E, Kim JS
  TITLE     Targeted chromosomal deletions in human cells using zinc finger 
            nucleases.
  JOURNAL   Genome Res. 2010 Jan;20(1):81-9. Epub 2009 Dec 1.
  PUBMED    19952142
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2010-01"; volume: "20(1)"; pages: "81-9. Epub 2009 Dec
            1"
COMMENT     Sequence Label: p3-K230-FokI_L (DAS)
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      82..91
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             94..120
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             94..120
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             130..150
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tgccaccatg gtgtacccct acgacgtgcc cgactacgcc
      121 gaattgcctc caaaaaagaa gagaaaggta gggatccgaa ttcccgggga aaaaccgtat
      181 gagtgtgatc actgtggaaa atcctttagc cagagctctc atctgaatgt gcacaaaaga
      241 actcacaccg gggaaaaacc gtataaatgc ggccagtgtg ggaagttcta ctcgcaggtc
      301 tcccacctca cccgccacca gaaaatccac accggggaaa aaccgtatga atgcgatcac
      361 tgtgggaaag ccttcagcgt cagctccaac ctgaacgtgc acagaagaat ccacaccggg
      421 gaaaaaccgt ataagtgccc tgattgtggg aagagtttta gtcagagttc cagcctcatt
      481 cgccaccagc ggacacacac cggtgaaaaa caactagtca aaagtgaact ggaggagaag
      541 aaatctgaac ttcgtcataa attgaaatat gtgcctcatg aatatattga attaattgaa
      601 attgccagaa atcccactca ggatagaatt cttgaaatga aggtaatgga attttttatg
      661 aaagtttatg gatatagagg tgagcatttg ggtggatcaa ggaaaccgga cggagcaatt
      721 tatactgtcg gatctcctat tgattacggt gtgatcgtgg atactaaagc ttatagcgga
      781 ggttataatc tgccaattgg ccaagcagat gccatgcaaa gctatgtcga agaaaatcaa
      841 acacgaaaca aacatatcaa ccctaatgaa tggtggaaa
//
