LOCUS       sequence-412951-        9703 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GAl4 response element driven NanoLuc Lentiviral reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9703)
  AUTHORS   Roennfeldt AE, Allen TP, Trowbridge BN, Beard MR, Whitelaw ML, 
            Russell DL, Bersten DC, Peet DJ
  TITLE     NanoFIRE: A NanoLuciferase and Fluorescent Integrated Reporter 
            Element for Robust and Sensitive Investigation of HIF and Other 
            Signalling Pathways.
  JOURNAL   Biomolecules. 2023 Oct 19;13(10):1545. doi: 10.3390/biom13101545.
  PUBMED    37892227
REFERENCE   2  (bases 1 to 9703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biomolecules."; date: "2023-10-19"; volume: "13(10):1545. doi"; 
            pages: " 10.3390/biom13101545"
COMMENT     Sequence Label: pLV-REPORT (PGK) 5xGRE-NanoLuc
FEATURES             Location/Qualifiers
     source          1..9703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    159..180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(195..200,201..218,219..225)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    233..249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     238..260
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     257..273
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     257..273
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     292..312
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        294..312
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        340..566
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             567..747
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    794..919
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1412..1645
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1830..1874
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2136..2253
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2401..2425
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2534..3046
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     CDS             3050..3169
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     primer_bind     complement(3217..3240)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3567..3586
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      3652..3661
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3673..3693
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3697..3717
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3721..3741
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3751..3753,3754..3756,3757..4467,4468..4473,
                     4474..4593)
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(3796..3817)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4057..4076)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4404..4425
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4594..4653
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             4654..5679
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4654..5679
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             6912..7145
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7217..7338
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7254..7273)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7308..7327
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7440..7459
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(7724..7742)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7810..7914
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7915..7983,7984..8775)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8133..8152)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8946..9534
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9435..9454
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgataaccct aattcgatag catatgcttc ccgttgggta acatatgcta ttgaattagg
       61 gttagtctgg atagtatata ctactacccg ggaagcatat gctacccgtt tagggttcac
      121 cggtgatgcc ggccacgatg cgtccggcgt agaggatcta atgtgagtta gctcactcat
      181 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      241 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
      301 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
      361 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
      421 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
      481 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
      541 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
      601 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      661 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      721 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
      781 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
      841 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
      901 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
      961 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     1021 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     1081 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1141 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     1201 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     1261 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1321 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1381 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1441 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1501 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1561 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1621 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1681 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     1741 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1801 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     1861 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     1921 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     1981 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2041 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2101 ccattcgatt agtgaacgga tctcgacggt atcggtttta aaagaaaagg ggggattggg
     2161 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     2221 ttacaaaaac aaattacaaa aattcaaaat tttatcgatg gtaccagctt gcatgcctgc
     2281 aggtcggagt actgtcctcc gagcctccga gcggagtact gtcctccgac ggagtactgt
     2341 cctccgagcg gagacggcgc gccagcttta ggcgtgtacg gtgggcgcct ataaaagcag
     2401 agctcgttta gtgaaccgtc agatcgcctg gagcaattcc acaacacttt tgtcttatac
     2461 caactttccg taccacttcc taccctcgta aagtcgacac cggggcccag atctatcgat
     2521 cggccggatc accatggtct tcacactcga agatttcgtt ggggactggc gacagacagc
     2581 cggctacaac ctggaccaag tccttgaaca gggaggtgtg tccagtttgt ttcagaatct
     2641 cggggtgtcc gtaactccga tccaaaggat tgtcctgagc ggtgaaaatg ggctgaagat
     2701 cgacatccat gtcatcatcc cgtatgaagg tctgagcggc gaccaaatgg gccagatcga
     2761 aaaaattttt aaggtggtgt accctgtgga tgatcatcac tttaaggtga tcctgcacta
     2821 tggcacactg gtaatcgacg gggttacgcc gaacatgatc gactatttcg gacggccgta
     2881 tgaaggcatc gccgtgttcg acggcaaaaa gatcactgta acagggaccc tgtggaacgg
     2941 caacaaaatt atcgacgagc gcctgatcaa ccccgacggc tccctgctgt tccgagtaac
     3001 catcaacgga gtgaccggct ggcggctgtg cgaacgcatt ctggcgaatt ctcacggctt
     3061 tccgcctgag gttgaagagc aagccgccgg tacattgcct atgtcctgcg cacaagaaag
     3121 cggtatggac cggcacccag ccgcttgtgc ttcagctcgc atcaacgtct aaaattctac
     3181 cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg
     3241 ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg
     3301 cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc
     3361 aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca
     3421 cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt
     3481 tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcaggg gcggggcggg
     3541 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc
     3601 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc atctcgaggt cgccaccatg
     3661 gtgcacgtgg atccaaaaaa gaagagaaag gtagatccaa aaaagaagag aaaggtagat
     3721 ccaaaaaaga agagaaaggt acacgtgtcc atggtgagca agggcgagga gctgttcacc
     3781 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     3841 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     3901 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     3961 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     4021 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     4081 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     4141 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     4201 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     4261 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     4321 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     4381 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     4441 actctcggca tggacgagct gtacaagaag cttagccatg gcttcccgcc ggaggtggag
     4501 gagcaggatg atggcacgct gcccatgtct tgtgcccagg agagcgggat ggaccgtcac
     4561 cctgcagcct gtgcttctgc taggatcaat gtgcagtgta ctaattatgc tctcttgaaa
     4621 ttggctggag atgttgagag caacccaggt cccatgaaaa agcctgaact caccgcgacg
     4681 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
     4741 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
     4801 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
     4861 gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat
     4921 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
     4981 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
     5041 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
     5101 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
     5161 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
     5221 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
     5281 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
     5341 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     5401 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     5461 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     5521 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     5581 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     5641 aaccgacgcc ccagcactcg tccgagggca aaggaatagg ctagcctagt gccatttgtt
     5701 cagtggttcg tagggctttc ccccactgtt tggctttcag ttatatggat gatgtggtat
     5761 tgggggccaa gtctgtacag catcttgagt ccctttttac cgctgttacc aattttcttt
     5821 tgtctttggg tatacattta aaccctaaca aaacaaagag atggggttac tctctaaatt
     5881 ttatgggtta tgtcattgga tgttatgggt ccttgccaca agaacacatc atacaaaaaa
     5941 tcaaagaatg ttttagaaaa cttcctatta acaggcctat tgattggaaa gtatgtcaac
     6001 gaattgtggg tcttttgggt tttgctgccc cttttacaca atgtggttat cctgcgttga
     6061 tgcctttgta tgcatgtatt caatctaagc aggctttcac tttctcgcca acttacaagg
     6121 cctttctgtg taaacaatac ctgaaccttt accccgttgc ccggcaacgg ccaggtctgt
     6181 gccaagtgtt tgctgacgca acccccactg gctggggctt ggtcatgggc catcagcgca
     6241 tgcgtggaac cttttcggct cctctgccga tccatactgc ggaactccta gccgcttgtt
     6301 ttgctcgcag caggtctgga gcaaacatta tcgggactga taactctgtt gtcctatccc
     6361 gcaaatatac atcgtttcca tggctgctag gctgtgctgc caactggatc ctgcgcggga
     6421 cgtcctttgt ttacgtcccg tcggcgctga atcctgcgga cgacccttct cggggtcgct
     6481 tgggactctc tcgtcccctt ctccgtctgc cgttccgacc gaccacgggg cgcacctctc
     6541 tttacgcgga ctccccgtct gtgccttctc atctgccgga ccgtgtgcac ttcgcttcac
     6601 ctctgcacgt cgcatggaga ccaccgtgaa cgcccaccaa atattgccca aggtcttaca
     6661 taagaggact cttggactct cagcaatgtc aacgaccgac cttgaggcat acttcaaaga
     6721 ctgtttgttt aaagactggg aggagttggg ggaggagatt aggttaaagg tctttgtact
     6781 aggaggctgt aggcataaat tggtctgcgc accagcacca tggcgcaatc actagagcgg
     6841 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     6901 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     6961 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7021 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     7081 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     7141 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     7201 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7261 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     7321 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     7381 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7441 atttatgcag aggccgaggc cggatccctt gagtggcttt catcctggag cagactttgc
     7501 agtctgtgga ctgcaacaca acattgcctt tatgtgtaac tcttggctga agctcttaca
     7561 ccaatgctgg gggacatgta cctcccaggg gcccaggaag actacgggag gctacaccaa
     7621 cgtcaatcag aggggcctgt gtagctaccg ataagcggac cctcaagagg gcattagcaa
     7681 tagtgtttat aaggccccct tgttaattct tgaagacgaa agggcctcgt gatacgccta
     7741 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     7801 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7861 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     7921 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     7981 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     8041 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     8101 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     8161 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     8221 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     8281 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     8341 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     8401 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     8461 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     8521 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     8581 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     8641 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     8701 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     8761 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     8821 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     8881 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     8941 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     9001 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     9061 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     9121 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     9181 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     9241 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     9301 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     9361 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     9421 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     9481 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     9541 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttgaagctg tccctgatgg
     9601 tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa
     9661 gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcg
//
